We narrowed to 17,203 results for: puro

1. pCSIIbsr-GCaMP6s

   Plasmid

   #184712

   Purpose

   Calcium biosensor

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   GCaMP6s
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. E.coli RaPID plasmid

   Plasmid

   #107251

   Purpose

   Expresses the E.coli RaPID labeling protein

   Depositor

   Paul Khavari

   Article

   Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

   Insert

   E.coli BirA*
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lentiCRISPR - AAVS1 sgRNA

   Plasmid

   #70661

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against AAVS1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

4. MyoD-pCLBabe

   Plasmid

   #20917

   Depositor

   Stephen Tapscott

   Article

   Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

   Insert

   MyoD (Myod1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pTRE-T2-IRES-His6-Ubiquitin

   Plasmid

   #107107

   Purpose

   Designed to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.

   Depositor

   Michael E. Wright

   Insert

   human ubiquitin UBB (UBB Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Tet

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPyCAG-Nanog-IP

   Plasmid

   #13838

   Depositor

   Shinya Yamanaka

   Article

   Mitsui et al Cell. 2003 May 30. 113(5):631-42.

   Insert

   Nanog (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Degron-KI p53 donor vector

   Plasmid

   #65619

   Purpose

   Donor vector to generate Degron-KI DD-p53

   Depositor

   Frank Stegmeier

   Article

   Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

   Insert

   p53 Degron-KI left and right HR arms
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 13, 2015

   Availability

   Academic Institutions and Nonprofits only

8. psd44-G13QL

   Plasmid

   #46828

   Purpose

   expression of mutant G protein alpha 13 in mammalian cells

   Depositor

   Agnese Mariotti

   Insert

   GNA13 (GNA13 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Q226L (The mutation reduces GTPase activity resul…

   Promoter

   PGK promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 4 gRNA concatemer

   Plasmid

   #84881

   Purpose

   Empty concatmer vector in which 4 gRNAs can be inserted

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEM705 Gso (Cys)

    Plasmid

    #109375

    Purpose

    GsX chimera with Gαo C-terminus (Pertussis toxin sensitive)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gso chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Cys at position 391 (Pertussis Toxin Sensitive)

    Promoter

    CAG

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLPC Flag-mRAP1

    Plasmid

    #73135

    Purpose

    Expression of Flag-tagged mRAP1

    Depositor

    Titia de Lange, Agnel Sfeir

    Article

    Sfeir et al Science. 2010 Mar 26;327(5973):1657-61.

    Insert

    Rap1 (Terf2ip Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKDR-Lenti-TetOn

    Plasmid

    #183555

    Purpose

    pKDR compatible TetOn lentiviral transfer plasmid

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO mouse shRNA 2 raptor

    Plasmid

    #21340

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 13. ():.

    Insert

    raptor shRNA 2 (Rptor Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pCAG-H2B-GFP

    Plasmid

    #184777

    Purpose

    Expresses H2B-GFP in human pluripotent stem cells

    Depositor

    Ivana Barbaric

    Article

    Price et al Curr Protoc. 2022 May;2(5):e435. doi: 10

    Insert

    H2BC11 (H2BC11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV.PARP1#1

    Plasmid

    #14544

    Depositor

    Didier Trono

    Article

    Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

    Insert

    PARP1 shRNA (PARP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pLJM1-PCYT2

    Plasmid

    #81074

    Purpose

    Expresses PCYT2 in mammalian cells

    Depositor

    Erin O'Shea

    Article

    Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

    Insert

    phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 2 gRNA concatmer

    Plasmid

    #84879

    Purpose

    Empty concatmer vector in which 2 gRNAs can be inserted

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pEM705 Gsi (Cys)

    Plasmid

    #109373

    Purpose

    GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsi chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Cys at position 391 (Pertussis Toxin Sensitive)

    Promoter

    CAG

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV.PARP1#2

    Plasmid

    #14545

    Depositor

    Didier Trono

    Article

    Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

    Insert

    PARP1 shRNA (PARP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

20. CLPIT Cry2PHR-mCh-LRP6c

    Plasmid

    #42961

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-LRP6c
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    LPR6 cytoplasmic domain only

    Promoter

    Tet-Off promoter

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TC1078-30

    Plasmid

    #164878

    Purpose

    30bp(mismatch17) cas13d gRNA for 1405 editing

    Depositor

    Tian Chi

    Article

    Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

    Insert

    gRNA for TC1405
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLI_TNF

    Plasmid

    #171179

    Purpose

    inducible expression of human TNF

    Depositor

    Veit Hornung

    Article

    Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

    Insert

    human TNF
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJPF0190

    Plasmid

    #130268

    Purpose

    Piggybac vector for the expression of mRuby2-fused human PRKACA

    Depositor

    Hana El-Samad

    Article

    Fonseca et al bioRxiv 2020, 747030

    Insert

    PRKACA (PRKACA Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. VPS35 shRNA1

    Plasmid

    #163858

    Purpose

    shRNA targeting the coding sequences of VPS35

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    VPS35 retromer complex component (VPS35 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p6643 MSCV-P C-FlagHA 31E7-Kozak

    Plasmid

    #35021

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV31 E7
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    March 6, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pTrans_9xD3B_Cluc_Norm

    Plasmid

    #68415

    Purpose

    CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Cypridina Luciferase-2A-Venus YFP
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    YFP: M153T,V163A,S175G ("Venus") YFP Va…

    Promoter

    minimalCMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p667-UBC-SCYL1-V5-miniTurbo_IDG-K

    Plasmid

    #189911

    Purpose

    Gateway destination clone of SCYL1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    SCYL1 (SCYL1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLPCX roGFP2-Prx2

    Plasmid

    #169230

    Purpose

    Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2 for cytosolic expression in mammalian cells (for stable cell line generation)

    Depositor

    Tobias Dick

    Article

    Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

    Insert

    roGFP2-Prx2
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLKO.1P shNFS1_1

    Plasmid

    #102963

    Purpose

    Suppression of NFS1

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shNFS1_1 (NFS1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p6647 MSCV-P C-Flag HA 2aE7

    Plasmid

    #35137

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV2a E7
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

31. p6649 MSCV-P C-FlagHA 38 E7-Kozak

    Plasmid

    #35143

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV38 E7
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

32. p6656 MSCV-P C-FlagHA 25E7-Kozak

    Plasmid

    #35022

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV25 E7
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    March 6, 2012

    Availability

    Academic Institutions and Nonprofits only

33. LV-TRE-VP16 mouse MyoD-T2A-dsRedExpress2

    Plasmid

    #60626

    Purpose

    Expresses VP16 mouse MyoD and dsRedExpress2 in response to doxycycline

    Depositor

    Charles Gersbach

    Article

    Kabadi et al ACS Synth Biol. 2014 Dec 10.

    Insert

    VP16 mouse MyoD
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBabe-GW-GFP

    Plasmid

    #40123

    Depositor

    Lynda Chin

    Article

    Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

35. pERB221

    Plasmid

    #61502

    Purpose

    const. CenpB-GFP-Halo + mCherry-DHFR

    Depositor

    Michael Lampson, David Chenoweth

    Article

    Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

    Inserts

    CenpB-GFP-Halo
    mCherry-DHFR
    

    Use

    Tags

    HaloTag and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEFIRES NQO1 WT

    Plasmid

    #61735

    Purpose

    Expresses WT human NQO1

    Depositor

    Yosef Shaul

    Article

    Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    EF1a

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. 3 gRNA concatemer

    Plasmid

    #84880

    Purpose

    Empty concatmer vector in which 3 gRNAs can be inserted

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

38. pDREV-2

    Plasmid

    #26752

    Depositor

    Rolf Zeller

    Article

    Osterwalder et al Nat Methods. 2010 Oct 17

    Insert

    H2B-Venus CDS (H2BC3 Human)
    

    Use

    Cre/Lox; Flp/frt; dre/rox

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pERB217

    Plasmid

    #61500

    Purpose

    Const. Halotag-GFP-Mito + mCherry-DHFR

    Depositor

    Michael Lampson, David Chenoweth

    Article

    Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

    Inserts

    HaloTag-GFP-Mito
    mCherry-DHFR
    

    Use

    Tags

    HaloTag and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p6895 PHAGE-P CMVT N-HA-hE6AP I wt

    Plasmid

    #37601

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP I wt (UBE3A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV tet regulatable

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge