We narrowed to 1,212 results for: Ncl

Showing: 691 - 720 of 1212 results

Results per page:

Sort results by:

pCCl-MCS_miniCMV_EGFP

Plasmid

#134984

Purpose

The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

EGFP

Use

Lentiviral

Promoter

minCMV

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ClCry4 PHR

Plasmid

#160552

Purpose

Expression of protein for various experiments including finding interaction partners

Depositor

Joseph Takahashi

Article

Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

Insert

Clcry4 HR

Tags

Hexa his

Expression

Insect

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN-Cherry-sgRNA

Plasmid

#221387

Purpose

episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

Depositor

Deborah Hung

Article

Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

Insert

none, cloning vector for introduction of targeting sequences

Use

CRISPR

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
JD639

Plasmid

#160398

Purpose

Binary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformation

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

35S::vitis miR396-resistant GRF4-GIF1 chimera

Use

Binary vector

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
JD690

Plasmid

#160397

Purpose

Binary vector pGWB14 including citrus GRF4-GIF1 under 35S promoter/ Plant transformation

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

35S::citrus GRF4-GIF1 chimera

Promoter

35S

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
JD631

Plasmid

#160399

Purpose

Binary vector pGWB14 including Vitis GRF4-GIF1 under 35S promoter/ Plant transformation

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

35S::vitis GRF4-GIF1 chimera

Use

Binary vector

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5-SUV39H2-FL

Plasmid

#89135

Purpose

mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase from pSG5, includes 5' and 3' noncoding regions

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

SUV39H2 (SUV39H2 Human)

Expression

Mammalian

Promoter

SV40

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pR26 GFP Dest

Plasmid

#74283

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mKate2-P2A-APEX2-csGBP

Plasmid

#108875

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mKate2-P2A-APEX2-csGBP

Expression

Mammalian

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-CAAX

Plasmid

#223558

Purpose

Plasmid expressing YFP(Venus) followed by the last 25 amino acids of human KRas4b including the polybasic regions and CAAX prenylation box

Depositor

Mikel Garcia-Marcos

Article

Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

Insert

YFP(Venus) - KRas4b

Expression

Mammalian

Available Since

March 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pR26 iBFP Dest

Plasmid

#74284

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and BFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
NLSx3-CRY2PHR-p65

Plasmid

#64124

Purpose

Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

CRY2PHR-p65

Tags

His, NLSx3, and V5

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Fus-BcLOV-mCh-SOS

Plasmid

#208275

Purpose

Expresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh-SOS

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-P2A-APEX2-ChBP

Plasmid

#108879

Purpose

Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-ChBP

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
1457_pDEST_miniTol2_R4-R2_Cryst_mCherry

Plasmid

#171796

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCCl-MCS_miniCMV_miRFP703

Plasmid

#134985

Purpose

The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

miRFP703

Use

Lentiviral

Promoter

minCMV

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Gata3-T2A-H2B-eGFP donor

Plasmid

#113119

Purpose

Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata3 donor region including homology arms and T2A-H2B-eGFP

Use

Synthetic Biology

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Fgfr2-T2A-H2B-Halo donor

Plasmid

#113117

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Fgfr2 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-P2A-APEX2-csChBP

Plasmid

#108877

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-csChBP

Expression

Mammalian

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCCl-MCS_miniCMV_TagBFP

Plasmid

#134986

Purpose

The plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

TagBFP

Use

Lentiviral

Promoter

minCMV

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiMutB3GNT5-blast

Plasmid

#208380

Purpose

lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

Depositor

Jennifer Kohler

Insert

Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)

Use

Lentiviral

Tags

myc, FLAG

Expression

Mammalian

Mutation

nt 922 c->g (PAM site inactivation)

Promoter

EF-1 alpha core promoter

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas1_Cas2/3

Plasmid

#89240

Purpose

Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

Cas1 and Cas2/3

Tags

Strep II tag on Cas1

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK255

Plasmid

#171155

Purpose

Expression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

I-SceI_sacB

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYF-sGFAP-IRES-tdTomato

Plasmid

#166920

Purpose

Empty plasmid backbone; includes IRES-tdTomato and sGFAP promoter

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Type

Empty backbone

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
UNC50-myc

Plasmid

#104382

Purpose

Expresses UNC50-myc wild-type in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-326

Insert

UNC50 (UNC50 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BcLOV-mCh-EGFR

Plasmid

#208274

Purpose

Expresses BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

BcLOV-mCherry-EGFR

Use

Lentiviral

Tags

mCherry

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH

Plasmid

#22930

Purpose

Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domain

Depositor

Alexandra Newton

Article

Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

Insert

PHLPP1-deltaPH (PHLPP1 Human)

Tags

HA

Expression

Mammalian

Mutation

Includes 127-1205 aa. Deleted amino-terminal PH d…

Promoter

CMV

Available Since

Feb. 3, 2010

Availability

Academic Institutions and Nonprofits only
pCB11

Plasmid

#201111

Purpose

pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.

Depositor

Mary Dunlop

Article

Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:

Inserts

CcaR
tetA(C)
sfGFP

Expression

Bacterial

Mutation

C-to-T transition at pos. no. 1049 of tetA(C) lea…

Promoter

J23100 and PcpcG2-172

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-EEA-g1-PGK-Puro

Plasmid

#102908

Purpose

PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

EEA-guide1-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS2356_Tier3(PB)-PuroR

Plasmid

#169655

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven PuroR expression

Expression

Mammalian

Promoter

PRPBSA

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge