-
Plasmid#212040PurposeBacterial expression of the CRISPRi system for genome editing in E. coliDepositorInsertddCpf1
UseCRISPRTagsExpressionMutationPromoterL-arabinose-inducible promoter PBADAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSF-CjPglB
Plasmid#199305PurposepSN18 derivative encoding C.jejuni PglB with C-terminal FLAG epitope tagDepositorInsertCjPglB with flag tag
UseTagsFlag tagExpressionBacterialMutationPromoterpBADAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sp_PE
Plasmid#212038PurposeBacterial expression of the PE2 proteins for genome editing in E. coliDepositorInsertPE2
UseCRISPRTagsExpressionMutationPromoterL-arabinose-inducible promoter PBADAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL2511
Plasmid#195625PurposeComplementation of MlaA(WT)DepositorInsertMlaA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACBSR-hyg
Plasmid#87830PurposeExpresses lambda-red system with hyg selection markerDepositorInserthyg
UseTagsNoneExpressionBacterialMutationNonePromoterblaAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCM184-ParB2::eGFP
Plasmid#87252PurposeExpresses ParB2-EGFP in yeast. Also known as pOR2-GFPDepositorInsertParB2
UseTagsEGFPExpressionYeastMutationPromoterpBAD/tetOAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCM189-ParB1::mCherry
Plasmid#87253PurposeExpresses ParB1-mCherry in yeast. Also known as pOR1-mchDepositorInsertParB1
UseTagsmCherryExpressionYeastMutationPromoterpBAD/tetOAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPerimCh
Plasmid#227013PurposeExpresses periplasmic mCherryDepositorInsertPelB-mCherry, arabinose inducible cytosolic GFP
UseTagsExpressionBacterialMutationPromoterrpsM and pBADAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
PylRS-AS
Plasmid#137908PurposeFor incorporation of fluorophenylalanine derivativesDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationContaining N346A, C348S mutationsPromoterpBADAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLNtC60αESC60β
Plasmid#160875PurposeExpression of N. tabacum Rubisco large subunit (rbcL), Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coliDepositorInsertrbcL, cpn60α, cpn60β, groES
UseTagsExpressionBacterialMutationPromoterrbcL: pBAD, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-link/link-C
Plasmid#61965PurposeNegative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)DepositorInsertsN-mCherry-link
link-C-mCherry
UseTagsExpressionBacterialMutationPromoterPBAD and PLtetOAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLNtC60αC20C60β
Plasmid#160874PurposeExpression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
UseTagsExpressionBacterialMutationPromoterrbcL: pBAD, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLAtC60αβC20
Plasmid#160873PurposeExpression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
UseTagsExpressionBacterialMutationPromoterrbcL: pBAD, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBA323
Plasmid#214751PurposeTo create delta(glmS,nadE) working strains and maintaining their viabilityDepositorInsertBBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVH016
Plasmid#80400PurposeNo anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).DepositorUseSynthetic BiologyTagsC-terminal 3xFLAG tag and C-terminal LZx domainExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3LCA_TM287/288
Plasmid#132701Purposeexpresses TM287/288 in E.coli, to use as positive control in ELISADepositorUseTagsAvi and HisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2f
Plasmid#105065PurposeA yellow fluorescent Ca2+ indicator variant with low binding affinity and fast kinetics for expression in bacterialDepositorInsertY-GECO2f
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+1D
Plasmid#149389PurposeP. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation1bp inserted downstream of the IHFprox sitePromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO1
Plasmid#55765PurposeEncoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in bacterial cellsDepositorInsertY-GECO1.0
UseLab constructedTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMHBAD14
Plasmid#42304DepositorInsertNpuDnaE C-intein
UseTagsGB1-H6ExpressionBacterialMutationPromoteraraBADAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2.2s
Plasmid#105069PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2.2s
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IRdist_scram
Plasmid#162319PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationThe distal Upstream Motif site is scrambledPromoterpBADAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-NgPglO
Plasmid#198204PurposepSN18 derivative encoding N. gonorrhoeae PglO with C-terminal FLAG epitope tagDepositorInsertNgPglO
UseTagsFlag tagExpressionBacterialMutationPromoterpBADAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2537
Plasmid#195658PurposeComplementation of MlaC(R153A)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(R153A)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2538
Plasmid#195659PurposeComplementation of MlaC(R186A)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(R186A)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2539
Plasmid#195660PurposeComplementation of MlaC(T116D)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(T116D)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2540
Plasmid#195661PurposeComplementation of MlaC(T176R)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(T176R)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2541
Plasmid#195662PurposeComplementation of MlaC(V68R)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(V68R)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2542
Plasmid#195663PurposeComplementation of MlaC(V135R)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(V135R)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2543
Plasmid#195664PurposeComplementation of MlaC(V146R)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(V146R)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2544
Plasmid#195665PurposeComplementation of MlaC(V171R)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(V171R)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2545
Plasmid#195666PurposeComplementation of MlaC(Y72A)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(Y72A)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2546
Plasmid#195667PurposeComplementation of MlaC(Y82K)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(Y82K)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2547
Plasmid#195668PurposeComplementation of MlaC(Y105A)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(Y105A)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2548
Plasmid#195669PurposeComplementation of MlaC(Y164R)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(Y164R)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2522
Plasmid#195644PurposeComplementation of MlaC(A108R)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(A108R)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2523
Plasmid#195645PurposeComplementation of MlaC(A163R)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(A163R)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2524
Plasmid#195646PurposeComplementation of MlaC(A168R)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(A168R)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2525
Plasmid#195647PurposeComplementation of MlaC(D165I)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(D165I)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2526
Plasmid#195648PurposeComplementation of MlaC(E169R)DepositorInsertMlaC
UseTagsExpressionBacterialMutationMlaC(E169R)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only