We narrowed to 13,937 results for: cas9

1. SpCas9 TadDE

   Plasmid

   #193837

   Purpose

   Express TadDE (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-DualEditor-Cas9D10A-2xUGI
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EFS-SpdCas9

   Plasmid

   #210703

   Purpose

   This plasmid express EFS promoter driven human codon optimized SpdCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   S. Pyogenes dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a core promoter

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9-CBE6d

   Plasmid

   #215822

   Purpose

   Express CBE6d (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6d-SpCas9-2xUGI (cas9 )
   

   Expression

   Mammalian

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHdzCas9-KRAB

   Plasmid

   #92341

   Purpose

   Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance

   Depositor

   I-Son Ng

   Article

   Kao et al Bioresour Technol. 2017 May 4. pii: S096

   Type

   Empty backbone

   Use

   CRISPR; Plant expression

   Tags

   KRAB

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-EF1adCas9VP64T2ABsd-W

   Plasmid

   #112919

   Purpose

   Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   dCas9VP64-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human EF1a promoter

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. xCas9 3.7

   Plasmid

   #108379

   Purpose

   Mammalian expression vector for xCas9 3.7

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9 3.7
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pnCas9-AID

   Plasmid

   #196242

   Purpose

   Plasmid expressing the nCas9-AID fusion

   Depositor

   Andrea Puhar

   Article

   Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

   Insert

   nickase Cas9 fused to AID
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdCas9-J23111

   Plasmid

   #113149

   Purpose

   Plasmid constitutively expressing dCas9 protein

   Depositor

   Chong Zhang

   Article

   Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

   Insert

   dCas9
   

   Expression

   Bacterial

   Mutation

   D10A & H840A

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9/pTREX-n

   Plasmid

   #68708

   Purpose

   Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

   Depositor

   Roberto Docampo

   Article

   Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

   Insert

   Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
   

   Use

   CRISPR

   Tags

   Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

   Mutation

   C4239T mutation that eliminates a BamHI restricti…

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEND-351_Cacnes_dCas9_CRISPRi

    Plasmid

    #225617

    Purpose

    Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458-HypaCas9

    Plasmid

    #190604

    Purpose

    PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. ACE2 g1 + Cas9

    Plasmid

    #153011

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP2

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPRx-Sth1Cas9-L5

    Plasmid

    #140993

    Purpose

    Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria

    Depositor

    Wilbert Bitter

    Article

    Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

    Inserts

    Sth1Cas9
    TetR
    L5 attP
    L5 Int
    KanR
    

    Use

    Unspecified; Integrative in mycobactreia

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYLCRISPR/Cas9Pubi-B

    Plasmid

    #66188

    Purpose

    expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    maize ubiquitin promoter (Pubi)

    Available Since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2_NBT:Cas9green; erbb2_gRNA171

    Plasmid

    #199337

    Purpose

    pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    NBT
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9(BB)-2A-miRFP670

    Plasmid

    #91854

    Purpose

    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

    Depositor

    Ralf Kuehn

    Inserts

    hSpCas9-2A-miRFP670
    BB-guide RNA
    

    Use

    CRISPR

    Tags

    2A-miRFP670 and 3XFLAG

    Expression

    Mammalian

    Promoter

    Cbh and U6

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pdCas9-humanized

    Plasmid

    #44246

    Purpose

    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dead Cas9 with 3X NLS
    

    Use

    CRISPR and Retroviral; Vector

    Tags

    3x NLS

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    MSCV LTR promoter

    Available Since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYLCRISPR/Cas9P35S-N

    Plasmid

    #66191

    Purpose

    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    cauliflower mosaic virus 35S promoter (P35S)

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdCas9- AsiA_m2.1

    Plasmid

    #158065

    Purpose

    Expressing evolved CasTA of dCas9 fused AsiA variant 2.1

    Depositor

    Harris Wang

    Article

    Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

    Insert

    dCas9-AsiA_m2.1
    

    Expression

    Bacterial

    Promoter

    TetO

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9-EGFP

    Plasmid

    #232761

    Purpose

    Expresses dCas9-EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    dCas9-EGFP
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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