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We narrowed to 9,096 results for: sgRNA

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Showing: 461 - 480 of 9096 results

  1. 7h sgRNA for 4-μHOM reporter

    Plasmid
    #113625
    Purpose
    sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.
    Depositor
    Jeremy Stark
    Publication
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    7h sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only

  2. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid
    #117687
    Purpose
    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.
    Depositor
    Erik Sontheimer
    Publication
    Gao et al Methods Enzymol. 2019;616:365-383. doi:
    Inserts
    Kanamycin cassette
    TetR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and hPGK
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    117687-plasmid-map-sequence-id-228342

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  4. lentiCRISPR - EGFP sgRNA 6

    Plasmid
    #51765
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 6
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51765-plasmid-map-sequence-id-79389

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  6. pLSB-KAN

    Plasmid
    #166700
    Purpose
    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Publication
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA Ser promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    166700-plasmid-map-sequence-id-329224

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  8. pUC57kan-T7-gRNA-U6 V2

    Plasmid
    #115520
    Purpose
    To construct multiple sgRNA expression vector for CRISPR
    Depositor
    Xingxu Huang
    Insert
    sgRNA scaffolf-U6 promoter
    Use
    CRISPR
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115520-plasmid-map-sequence-id-224556

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  10. U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

    Plasmid
    #92120
    Purpose
    Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MS2-P65-HSF1 (HSF1 Synthetic, Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92120-plasmid-map-sequence-id-197614

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  12. pLSB-HYG

    Plasmid
    #166699
    Purpose
    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Publication
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166699-plasmid-map-sequence-id-474294

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  13. pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

    Plasmid
    #148859
    Purpose
    Mammalian Expression of HsXRN1-sgRNA
    Depositor
    Elisa Izaurralde
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HsXRN1-sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148859-plasmid-map-sequence-id-478935

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  19. pmCherry_sgRNA-ver2

    Plasmid
    #126776
    Purpose
    gRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNA
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA (for SpCas9)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126776-plasmid-map-sequence-id-412379

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  20. pLSB-NAT

    Plasmid
    #166698
    Purpose
    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Publication
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    166698-plasmid-map-sequence-id-329407

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Showing: 461 - 480 of 9096 results