We narrowed to 26,741 results for: STI

1. pDONR P2R-P3-ECFP

   Plasmid

   #48353

   Purpose

   Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   ECFP
   

   Use

   Gateway entry vector

   Mutation

   Contains a stop codon.

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-mTcte1-PA

   Plasmid

   #176469

   Purpose

   Expression vector of mouse t-complex-associated testis expressed 1 (Tcte1) tagged with PA at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

   Insert

   t-complex-associated testis expressed 1 (Tcte1 Mouse)
   

   Tags

   PA

   Expression

   Mammalian

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW-TetO-Dppa2

   Plasmid

   #40799

   Depositor

   Rudolf Jaenisch

   Article

   Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

   Insert

   Dppa2 (Dppa2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Minimal CMV + TetO

   Available Since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pcDNATM3.1/V5-His TOPO-PKCi-K274W

   Plasmid

   #55690

   Purpose

   expresses dominant negative human PKCi tagged with V5

   Depositor

   Pedro Salas

   Article

   Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

   Insert

   Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   A120E and K274W

   Promoter

   CMV

   Available Since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pYPQ230-RVQ

   Plasmid

   #200187

   Purpose

   Gateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editing

   Depositor

   Yiping Qi

   Article

   Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

   Insert

   LbCas12a-RVQ
   

   Use

   CRISPR; Gateway compatible lbcas12a-rv entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   G146R, R182V, E795Q

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR P4-P1R-EYFP

   Plasmid

   #48350

   Purpose

   Contributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   EYFP
   

   Use

   Gateway entry vector

   Mutation

   Contains a Kozak sequence. Does not contain a sto…

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMP71-mIL15-IL12rb1-sushi-il12rb2

   Plasmid

   #174606

   Purpose

   Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-dSaCas9-KRAB-blast

   Plasmid

   #191654

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   dSaCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLin28A-myc-T2A-tagRFP

   Plasmid

   #140267

   Purpose

   Expresses LIN28A in mammalian cells

   Depositor

   Hirohide Saito

   Article

   Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

   Insert

   LIN28A (LIN28A Human)
   

   Use

   Synthetic Biology

   Tags

   myc-T2A-tagRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-CIBN-EGFP-KRasCT

    Plasmid

    #178527

    Purpose

    Expresses CIBN-EGFP-KRasCT in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    CIBN-EGFP-KRasCT
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs_FLAG-SFXN1

    Plasmid

    #110634

    Purpose

    Retroviral expression of Flag-SFXN1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN1 (SFXN1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

    Plasmid

    #72888

    Purpose

    fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein (luminopsin) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

    Insert

    Gaussia luciferase, Channelrhodopsin-2, EYFP
    

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cav2.2e[a10,Δ18a, Δ24a, 31a, 37b, 46]

    Plasmid

    #26567

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Contains exons a10, 31a, 37b, and 46. Deletion…

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pWPT-mCherry

    Plasmid

    #190605

    Purpose

    Obtained by cloning 5 stop codons in place of the EGFP Kozak sequence in pWPT-/mEGFP-1T-IRES-mCherry

    Depositor

    Alessandro Quattrone

    Article

    Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral

    Mutation

    EGFP Kozak sequence has been replaced with a stre…

    Promoter

    EIF1-short

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP in pDBEcR-pIND(SP1)/Bla

    Plasmid

    #201557

    Purpose

    Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.

    Depositor

    Randy Poon

    Article

    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

    Insert

    DBEcR and EGFP
    

    Use

    Unspecified; Sleeping beauty transposon

    Tags

    DBEcR-myc and FLAG-EGFP

    Expression

    Mammalian

    Promoter

    EF1a and pIMD(SP1)

    Available Since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMCS.71/2272

    Plasmid

    #22732

    Depositor

    Mark Magnuson

    Article

    Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

    Insert

    Lox71-MCS-Lox2272
    

    Use

    Cre/Lox

    Mutation

    This is a basal exchange vector for RMCE containi…

    Available Since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. WTTEM1_pSTC0-zeo

    Plasmid

    #202351

    Purpose

    Control WT TEM-1 (wild type TEM-1 beta lactamase) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    TEM-1
    

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. DMP1-GFPtopaz

    Plasmid

    #114270

    Purpose

    GFP labeling of mature osteoblasts and osteocytes.

    Depositor

    David Rowe

    Article

    Kalajzic et al Bone. 2004 Jul;35(1):74-82. doi: 10.1016

    Insert

    Dmp1 + GFPtopaz (Dmp1 Mouse, Aequorea victoria)
    

    Expression

    Mammalian

    Promoter

    Dmp1

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDF11-SFPQ(276-598)

    Plasmid

    #135437

    Purpose

    Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    Splicing Factor Proline And Glutamine Rich (SFPQ Human)
    

    Tags

    hexahistidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-FOXO3a TM delta CT

    Plasmid

    #1797

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A, delta CT (aa 468-673)

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

21. px330 NIPBL gRNA

    Plasmid

    #165590

    Purpose

    Insertion of NIPBL degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    NIPBL (Nipbl Mouse)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]

    Plasmid

    #26569

    Depositor

    Diane Lipscombe

    Article

    Bell et al Neuron. 2004 Jan 8. 41(1):127-38.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 24a. Contains …

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pET28-mTDGa.0

    Plasmid

    #81050

    Purpose

    bacterial expression of murine TDG

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TDG (Tdg Mouse)
    

    Tags

    6HIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mCherry-Climp63(1–192)

    Plasmid

    #186955

    Purpose

    Expresses mCherry-Climp63 with partial luminal domain

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Ckap4 (Ckap4 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only remain partial luminal domain

    Promoter

    CMV Promoter

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV Reep3-V5

    Plasmid

    #175119

    Purpose

    Lentiviral expression of mouse Reep3-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Reep3 (Reep3 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W

    Plasmid

    #200475

    Purpose

    Lentiviral vector expressing gRNA targeting GFP

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.2/NS5-Mt-mut

    Plasmid

    #120830

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    ZIKV NS5 E218A "Mtase mut"
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    E218A

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOPS0378

    Plasmid

    #133229

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. YFP Rab4DN

    Plasmid

    #99019

    Purpose

    Localization and visualization of the dominant negative form of Rab4

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

    Insert

    Rab4 DN (Rab4 Synthetic, Fly)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    S22N Dominant negative

    Promoter

    CMV

    Available Since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Cav2.2e[Δa10, Δ18a, 24a, Δ31a, 37b, 46]

    Plasmid

    #26571

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 31a Contains e…

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

31. pINDUCER21-puro_TAOK2

    Plasmid

    #172979

    Purpose

    doxycycline-inducible expression of TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    TAOK2 (TAOK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLX302 IL10RA-V5 puro

    Plasmid

    #47552

    Purpose

    Lentiviral expression of V5-tagged IL10RA in mammalian cells

    Depositor

    Kevin Janes

    Article

    Kang et al Sci Signal. 2013 Aug 6;6(287):rs13. doi:

    Insert

    IL10RA (IL10RA Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

33. PBLO CasX-gRNA

    Plasmid

    #126419

    Purpose

    E. coli vector for CasX gRNA --- Used for In Vitro Transcription

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    casX gRNA
    

    Use

    CRISPR; In vitro transcription

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3 PBD wt (Nigg AH12)

    Plasmid

    #41162

    Depositor

    Erich Nigg

    Article

    Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

    Insert

    PBD (PLK1 Human)
    

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    Contains Plk1-PBD only (aa 326–603)

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

35. EYFP-IKB

    Plasmid

    #111194

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

    Insert

    IKB-alpha
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLentiguide-puro_hORAI1sg

    Plasmid

    #174326

    Purpose

    gRNA targeting hORAI1 - encodes puromycin resistance

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human Orai1 (ORAI1 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pB.FRB-3myc-KLC1a

    Plasmid

    #134627

    Purpose

    Mammalian expression of mouse KLC1a

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KLC1a (Klc1 Mouse)
    

    Tags

    FRB, 3myc

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Chicken Beta actin

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

    Plasmid

    #204288

    Purpose

    yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
    

    Tags

    mRuby3

    Expression

    Yeast

    Promoter

    pHis3

    Available Since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HA-FOXO3a T32A DBM (H212R)

    Plasmid

    #1793

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A, H212R

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

40. pLV-REPORT (PGK) 5xGRE-NanoLuc

    Plasmid

    #207170

    Purpose

    GAl4 response element driven NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Roennfeldt et al Biomolecules. 2023 Oct 19;13(10):1545. d

    Inserts

    NanoLuciferase
    d2eGFP
    5x GRE
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Promoter

    Gal4 response element minP and PGK

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge