Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 44,705 results for: cha;

Clear keywords
Showing: 9561 - 9600 of 44705 results

  4. TR- ATG-HP-GFP

    Plasmid
    #80592
    Purpose
    Expresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
    Depositor
    Aravind Asokan
    Article
    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12
    Insert
    GFP
    Use
    AAV
    Expression
    Mammalian
    Mutation
    Inserted 30 nt hairpin which is targeted by Csy4 …
    Promoter
    CBA
    Available Since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    80592-plasmid-map-sequence-id-151877

    Enlarge

  5. pMpGWB125

    Plasmid
    #68579
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Triple repeats of Citrine (3xCitrine)
    Expression
    Plant
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68579-plasmid-map-sequence-id-122049

    Enlarge

  6. pGEX6P1‐Afu‐rnhBΔPIP

    Plasmid
    #108696
    Purpose
    For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion
    Depositor
    Andrew Jackson, Martin Reijns
    Article
    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6
    Insert
    rnhB
    Tags
    GST
    Expression
    Bacterial
    Mutation
    C-terminal truncation (7aa) removing PIP box
    Promoter
    tac
    Available Since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    108696-plasmid-map-sequence-id-208999

    Enlarge

  10. pMpGWB118

    Plasmid
    #68572
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    MpEF1alpha promoter
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68572-plasmid-map-sequence-id-121810

    Enlarge

  12. pJEC626

    Plasmid
    #174367
    Purpose
    Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.
    Depositor
    James Chappell
    Article
    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793
    Insert
    Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23106
    Available Since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174367-plasmid-map-sequence-id-345703

    Enlarge

  13. pAAV-CaMKIIa-ChRger1-TS-EYFP

    Plasmid
    #127244
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger1-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Promoter
    CamKIIa
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127244-plasmid-map-sequence-id-247231

    Enlarge

  16. pMpGWB218

    Plasmid
    #68609
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    MpEF1alpha promoter
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68609-plasmid-map-sequence-id-121811

    Enlarge

  21. pWL502-PAM

    Plasmid
    #174382
    Purpose
    Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    PAM-spacer
    Use
    CRISPR; Archaeal expression
    Expression
    Bacterial
    Available Since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174382-plasmid-map-sequence-id-344136

    Enlarge

  36. pMpGWB414

    Plasmid
    #68679
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Glucocorticoid receptor (GR)
    Expression
    Plant
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68679-plasmid-map-sequence-id-121733

    Enlarge

  40. pCALNL_chr13_102010574-102010650

    Plasmid
    #81213
    Purpose
    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38)
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    gix-Neo-gix deletion cassette upstream of EGFP
    Expression
    Mammalian
    Promoter
    pCBa
    Available Since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81213-plasmid-map-sequence-id-163268

    Enlarge

Showing: 9561 - 9600 of 44705 results