We narrowed to 7,491 results for: RAP
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Plasmid#129594PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
Tags6xHis-tagExpressionBacterialPromoterT7Available SinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET23b-15F11-HA-mEGFP-6xHis
Plasmid#129593PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.DepositorInsertAnti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
Tags6xHis-tagExpressionBacterialPromoterT7Available SinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-Anillin
Plasmid#68027PurposeFull length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1DepositorInsertAnillin (ANLN Human)
TagseGFPExpressionMammalianMutationsilent mutations at 798 5'-TGCC TCTTTGAATAAA…PromoterCMVAvailable SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE mNeonGreen-Core (HBc from HBV) IRES puro
Plasmid#122202PurposePlasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.DepositorInsertsHBc from HBV AYW
mNeonGreen
TagsGGSGGSGGS linkerExpressionMammalianAvailable SinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-Lck-FRB-mTurquoise2
Plasmid#176130PurposePlasma membrane localized FRB, rapamycin heterodimerizationDepositorInsertFRB
UseLentiviralAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RhoA Biosensor
Plasmid#68026PurposeC-terminus of Anillin in mammalian expression vector pEGFP-C1DepositorAvailable SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-CD151-7
Plasmid#55282PurposeLocalization: Tetraspanin/Membrane, Excitation: 399, Emission: 456DepositorAvailable SinceOct. 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRISPR-cBEST
Plasmid#125689PurposeC to T base editor for actinomycetesDepositorInsertstreptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
UseCRISPR and Synthetic BiologyPromoterermE*/tipAAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30a-GABAT
Plasmid#239572PurposeExpresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.DepositorAvailable SinceJuly 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
Plasmid#137830PurposeMESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factorDepositorInsertFRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
Tags3x FLAGExpressionMammalianMutationMutated Leucine 71 to Lysine in CTEVp fragmentPromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXL001-TQM IL13 CAR
Plasmid#154054PurposeLentiviral expression of TQM IL13 CAR for CAR-T therapyDepositorInsertTQM IL13 CAR
UseLentiviralPromoterEF1aAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-HiBiT-CD9
Plasmid#162592PurposeN-terminally HiBiT-tagged human CD9 under CAG promoterDepositorAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-FKBP-GEF(TIAM1)
Plasmid#85156Purposeacute activation of Rac through rapamycin-induced translocation to membrane-localized FRBDepositorInsertFusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)
TagsmCherry-FKBPExpressionMammalianPromoterCAGAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJW1659
Plasmid#154314PurposeNT Donor-cassette for SapTrapDepositorInsert30aa linker::AID*::3xFLAG for NT slot
UseCRISPRExpressionWormAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 ΔEMV-6MT
Plasmid#41737DepositorInsertNotch-1 Extracellular-domain-deleted construct (Notch1 Mouse)
Tags6xMycExpressionMammalianMutationContains murine Notch-1 protein fragment lacking …Available SinceJan. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_VHH-GFP4::CD8::mCherry
Plasmid#163917PurposeExpression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancersDepositorInsertFusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJW1816
Plasmid#154320PurposeMulti-cassette for SapTrapDepositorInsert30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc
UseCRISPR and Cre/LoxExpressionWormAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(H75E)
Plasmid#137829PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(H75E)
Tags3x FLAGExpressionMammalianMutationMutated Histidine 75 to Glutamic Acid in NTEVp fr…PromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL3-deltaTM
Plasmid#109025Purposestable lentiviral expression of cDNADepositorInsertATL3-deltaTM (ATL3 Human)
UseLentiviralTagseGFPExpressionMammalianMutationtruncated form without C-terminal ER transmembran…PromoterEIF-1a promoterAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1347
Plasmid#154310PurposeDonor-cassette for SapTrapDepositorInsert30aa Linker for CT slot
UseCRISPRExpressionWormAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
LLP783_SpyCatcher-DNMT3A-CO
Plasmid#211776PurposeSpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavudin-DNMT3A
Tags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SKAP2-1/1
Plasmid#91447PurposeProtein expression and purification of human SH3 domain construct SKAP2-1/1DepositorInsertSKAP2-1/1 (SKAP2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL1
Plasmid#109018Purposestable lentiviral expression of cDNADepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAN201
Plasmid#129204PurposeyTRAP sensor of [RNQ+], detects aggregation of Rnq1DepositorAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRB-mCherry-EEA1
Plasmid#222419PurposeTo express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cellsDepositorAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT25_GalL_BE3
Plasmid#143736PurposeExpresses BE3 in yeast cellsDepositorInsertBE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB80-FKBP-mCherry-RAB11
Plasmid#174622PurposeRapalog-inducible coupling to recycling endosomes (RAB11) via FKBPDepositorInsertFKBP-mCherry-RAB11 (RAB11A Human, Synthetic)
TagsFKBP-mCherryExpressionMammalianMutationmCherry: Met1Del; RAB11: Met1DelPromoterChicken beta-actinAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAN200
Plasmid#129203PurposeyTRAP sensor of [PSI+], detects aggregation of Sup35DepositorInsertSup35NM (SUP35 Budding Yeast)
UseSynthetic BiologyExpressionYeastMutationN terminal and middle domainsPromoterSUP35Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCA528 SPASTIN , residues 112-196
Plasmid#180627PurposeBacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.DepositorInsertSPASTIN
TagsHIS-SUMOExpressionBacterialMutationResidues 112-196PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only