We narrowed to 13,950 results for: UNG

1. pHAGE-EFS-MCP-HALOnls

   Plasmid

   #121937

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   MCP-HALOnls
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pESC-leu2d(empty)

   Plasmid

   #20120

   Depositor

   Jay Keasling

   Article

   Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. bZiPro

   Plasmid

   #86846

   Purpose

   express NS2B-NS3 protease in E.coli

   Depositor

   Dahai Luo

   Article

   Zhang et al Science. 2016 Dec 8. pii: aai9309.

   Inserts

   NS3 1-177
   NS2B45-96
   

   Use

   Tags

   a hexahistidine tag and a thrombin cleavage site

   Expression

   Bacterial

   Mutation

   NS2B cofactor region (residues 45–96) and NS3 pro…

   Promoter

   T7

   Available since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPUR-hU6-sgRNA-Sirius-8XMS2

   Plasmid

   #121942

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   sgRNA-Sirius-8XMS2
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSELIS-RamR

   Plasmid

   #202631

   Purpose

   Enables dual growth and fluorescence based enrichment of RamR biosensors

   Depositor

   Andrew Ellington

   Article

   d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

   Insert

   Sh ble
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lambda promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-CA-rtTA Adv

   Plasmid

   #20910

   Purpose

   piggyBac vector for expression of reverse tetracycline transactivator from a CAG constitutive promoter

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   rtTA-ON-Adv
   

   Use

   piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEvol-NnSULT1C1-cysDNCQ

   Plasmid

   #188983

   Purpose

   biosynthesize sulfotyrosine in E. coli.

   Depositor

   Han Xiao

   Article

   Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

   Inserts

   NnSULT1C1
   cysD
   cysN
   cysC
   cysQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-ERK-SPARK

   Plasmid

   #106921

   Purpose

   Express ERK-SPARK, an activity reporter of ERK in mammalian cells.

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   ERK-SPARK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pgRNA-modified

   Plasmid

   #84477

   Purpose

   guide RNA expression lentiviral construct

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   guide RNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-PKA-SPARK

    Plasmid

    #106920

    Purpose

    Express PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    PKA-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET16better_H10_HRV3C_chitinase

    Plasmid

    #175091

    Purpose

    Expresses B. licheniformis chitinase with an N terminal 10X His tag and HRV-3C cleavage site in E. coli.

    Depositor

    Jay C Dunlap

    Article

    Bartholomai et al Fungal Genet Biol. 2021 Aug 20;156:10361

    Insert

    chitinase
    

    Use

    Tags

    10X His, HRV-3C

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX-EF1a_NKX2-1_Long

    Plasmid

    #221483

    Purpose

    NKX2-1 long isoform lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1P shSOD2

    Plasmid

    #102976

    Purpose

    Suppression of SOD2

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shSOD2 (SOD2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGM9121_pALD4

    Plasmid

    #160919

    Purpose

    Mitochondrial aldehyde dehydrogenase (ALD4) promoter module for episomal P. pastoris promoter shuffling

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

    Insert

    Mitochondrial aldehyde dehydrogenase promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. BSA1_His

    Plasmid

    #202766

    Purpose

    bovine serum albumin design BSA1, in pET29b with C-terminal 6His-tag

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

    Insert

    BSA1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TALED_Left-stuffer-1397C-8e

    Plasmid

    #187169

    Purpose

    original vector to make all constructs

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    Left-stuffer-1397C-8e
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. TALED_Right-stuffer-1397N-UGI

    Plasmid

    #187173

    Purpose

    original vector to make all constructs

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    Right-stuffer-1397N-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-hphMX6-81nmt1

    Plasmid

    #105164

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    hphMX6-81nmt1
    

    Use

    Tags

    81nmt1

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    81nmt1

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA6a-hphMX6-41nmt1

    Plasmid

    #105163

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    hphMX6-41nmt1
    

    Use

    Tags

    41nmt1

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-hphMX6-3nmt1

    Plasmid

    #105162

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    hphMX6-3nmt1
    

    Use

    Tags

    3nmt1

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    3nmt1

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV-G-Cdc42

    Plasmid

    #180577

    Purpose

    ddGFP sensor of Cdc42

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddGFP sensor of Cdc42
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMV-R-Cdc42

    Plasmid

    #180578

    Purpose

    ddRFP sensor of Cdc42

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddRFP sensor of Cdc42
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-EFS-PCP-GFPnls

    Plasmid

    #121938

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    PCP-GFPnls
    

    Use

    Lentiviral

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pME-mTagBFP

    Plasmid

    #75148

    Purpose

    TagBFP (blue) fluorophore for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mTagBFP
    

    Use

    Middle entry vector for multisite gateway three f…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOO2-GW

    Plasmid

    #135097

    Purpose

    Gateway vector for expression of proteins in Xenopus oocytes

    Depositor

    Wolf Frommer

    Article

    Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

    Type

    Empty backbone

    Use

    Bacterial expression vector for generating sp6 tr…

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3.1-mMaroon1

    Plasmid

    #83840

    Purpose

    Expresses mMaroon1

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pC0041-RanCas13b crRNA backbone

    Plasmid

    #103852

    Purpose

    For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-RanCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJJB302

    Plasmid

    #107694

    Purpose

    OsActin Promoter-LwaCas13a-HSP Terminator

    Depositor

    Daniel Voytas

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    Cas13a
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsActin

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pUBC_V5_MS2-mCherry

    Plasmid

    #68420

    Purpose

    Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    MS2
    

    Use

    Synthetic Biology

    Tags

    V5 and mCherry

    Expression

    Mammalian

    Mutation

    MS2: V75E/A81G

    Promoter

    human UbC

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCS2.aBTX

    Plasmid

    #122257

    Purpose

    Express membrane-tethered alpha-bungarotoxin in Danio rerio

    Depositor

    Loren Looger

    Article

    Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

    Insert

    alpha-bungarotoxin
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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