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pYPQ136B2.0

Plasmid

#167160

Purpose

Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Ptet_P

Plasmid

#127005

Purpose

Basic Part P- Promoter (non-constitutive); Ptet; induced by tetracycline/analogue; controlled by TetR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Ptet_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAM5057

Plasmid

#120085

Purpose

Broad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.

Depositor

Susan Golden

Article

Ma et al Appl Environ Microbiol. 2014 Nov;80(21):

Insert

RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter

Use

Other

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCAP05

Plasmid

#102920

Purpose

Direct cloning and broad-host-range heterologous expression vector

Depositor

Bradley Moore

Article

Zhang et al MBio. 2017 Sep 5;8(5). pii: e01291-17. d

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-mCh-kCAAX

Plasmid

#112267

Purpose

ITRACK Acceptor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

mCherry with KRas membrane targeting domain

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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JGI_018319

Plasmid

#158837

Purpose

Rare earth element alcohol dehydrogenases

Depositor

Joint Genome Institute

Article

Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

Insert

Mex_1740

Use

Synthetic Biology

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pQFT-mNGsacB

Plasmid

#203676

Purpose

pQFT with mNeonGreen and sacB under control of PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Inserts

mNeonGreen
sacB

Expression

Bacterial

Mutation

Has synonymous (silent) mutations to remove BsrGI…

Promoter

PQJ

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pSW002

Plasmid

#111248

Purpose

Broad host-range bacterial vector

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCLE NICD3

Plasmid

#26894

Depositor

Nicholas Gaiano

Article

Dang et al Dev Neurosci. 2006;28(1-2):58-69.

Insert

NICD3 (NOTCH3 Human)

Use

Retroviral

Tags

Alkaline Phosphatase and IRES

Expression

Mammalian

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pLEW100cre-EP1

Plasmid

#33345

Purpose

For expression of tetracycline-inducible CRE in Trypanosoma brucei

Depositor

George Cross

Article

Scahill et al Mol Biochem Parasitol. 2008 Jan;157(1):7

Insert

cre recombinase

Use

Cre/Lox; Trypanosoma brucei expression vector

Promoter

TET operator

Available Since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only

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pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

Plasmid

#45565

Purpose

Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

I-Sce I Nuclease
BFP

Tags

HA

Expression

Mammalian

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR APLP1 shRNA 2

Plasmid

#30157

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP1 shRNA 2

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-pCAG-Floxed-SynGFP,rev.-WPRE

Plasmid

#234892

Purpose

CAG-driven, Cre-dependent synaptophysin-tagged GFP for anterograde tracing of neuronal projections

Depositor

Silvia Arber

Article

Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

Insert

Floxed-SynGFP

Use

AAV

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB3-PUM1-HD

Plasmid

#17543

Depositor

Traci Hall

Article

Wang et al Mol Cell. 2001 Apr . 7(4):855-65.

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only
pPI356

Plasmid

#207449

Purpose

Extrachromosomal expression vector PkgE-PH-GFP and PTEN-mCherry

Depositor

Rob Kay

Article

Lutton et al Curr Biol. 2023 Aug 7;33(15):3083-3096.e

Inserts

PkgE
PTEN

Use

Unspecified

Tags

GFP and mCherry

Mutation

PH domain only

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pPRIME-TET-GFP-recipient

Plasmid

#11655

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pNG6

Plasmid

#30990

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NG6

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pUC_sgRNA

Plasmid

#68710

Purpose

Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Available Since

Sept. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCAX APP C60

Plasmid

#30152

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein C60

Expression

Mammalian

Mutation

fragment

Available Since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only
pSECRETS-A

Plasmid

#196986

Purpose

For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

Depositor

Eric Josephs

Article

Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pltetO1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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