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pU6-Sox3gRNA-T1

Plasmid

#127777

Purpose

Chicken sox3 gRNA target site 1, cloned into the pU6 sgRNA (backbone #92359)

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

sox3gRNAT1

Use

CRISPR

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Ptet_P

Plasmid

#127005

Purpose

Basic Part P- Promoter (non-constitutive); Ptet; induced by tetracycline/analogue; controlled by TetR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Ptet_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pPD_Pinx-1(1k + 50 bp)::Venus

Plasmid

#117421

Purpose

For analysis of AIB cell differentiation by tracking Venus fluorescence under the expression of Pinx-1 promoter

Depositor

Shohei Mitani

Article

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

Venus

Expression

Worm

Promoter

inx-1

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pstb-LAFR5-exoY

Plasmid

#86170

Purpose

pstb-LAFR5 with the S. meliloti 1021 exoY fragment from E6 cloned into Pst/Bam

Depositor

Kay Jones

Article

Jones et al J Bacteriol. 2012 Aug;194(16):4322-31. d

Insert

exoY

Expression

Bacterial

Promoter

Smb21651

Available Since

Feb. 6, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pME-Fv2E-flt4-ns

Plasmid

#67695

Purpose

middle entry plasmid with dimerizable extracellular domain fused to Danio Flt4 cytoplasmic domain

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4

Mutation

no stop codon; D1302E (see depositor's comme…

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only
pCVD518

Plasmid

#66158

Purpose

Six copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1, for generation of probe

Depositor

James Kaper

Article

Nishibuchi et al Infect Immun. 1985 Sep;49(3):481-6.

Insert

tdh1

Use

Cloning vector

Promoter

Unknown

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pCVD402

Plasmid

#66148

Purpose

to make a probe to detect Heat stable enterotoxin gene (human)

Depositor

James Kaper

Article

Nishibuchi et al J Clin Microbiol. 1988 Apr;26(4):784-6.

Insert

STh

Use

Cloning vector

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pASKt15C+SrtA2woH

Plasmid

#65018

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Promoter

tetracycline promoter/operator

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pNH094

Plasmid

#42167

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1-RT-::[(G4S)3]::C-TAP Tag

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR APLP1 shRNA 3

Plasmid

#30158

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP1 shRNA 3

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pHD8

Plasmid

#30981

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat HD8

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pNG5

Plasmid

#30989

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NG5

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pNI6

Plasmid

#31001

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NI6

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pNN1

Plasmid

#31007

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NN1

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pNN3

Plasmid

#31009

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NN3

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pNN4

Plasmid

#31010

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NN4

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pNN5

Plasmid

#31011

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NN5

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pNN7

Plasmid

#31013

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NN7

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pNN8

Plasmid

#31014

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NN8

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pNK1

Plasmid

#31036

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NK1

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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