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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,304 results for: construct

Showing: 4841 - 4860 of 8304 results

  6. pCLAP

    Plasmid
    #200786
    Purpose
    Cloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.
    Depositor
    Alison Twelvetrees
    Article
    Smith et al bioRxiv 2024.12.20.629623
    Type
    Empty backbone
    Use
    Tags
    CLIPf and SNAPf-6xHis-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    200786-plasmid-map-sequence-id-435692

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  7. Rab11A[8-175]-His-C Q70L

    Plasmid
    #228042
    Purpose
    Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling. Constitutively active mutation (Q70L).
    Depositor
    Wade Harper
    Insert
    Rab11A[8-175]-His-C Q70L (RAB11A Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Q70L
    Promoter
    Available since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    228042-plasmid-map-sequence-id-450388

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  13. pYTK001_P2Ab

    Plasmid
    #183084
    Purpose
    2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly
    Depositor
    Zhen Wang
    Article
    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.
    Insert
    P2A
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    183084-plasmid-map-sequence-id-367243

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  15. pMbd3EosHaloTAF_Hyg

    Plasmid
    #140958
    Purpose
    Targeting construct to make Mbd3-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    methyl-CpG binding protein 3 (Mbd3 Synthetic, Mouse)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Expression
    Mutation
    Promoter
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    140958-plasmid-map-sequence-id-338424

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  16. pChd4EosHaloTAF_Puro

    Plasmid
    #172099
    Purpose
    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    chromodomain helicase DNA binding protein 4 (Chd4 Synthetic, Mouse)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Expression
    Mutation
    Promoter
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    172099-plasmid-map-sequence-id-338425

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  18. pPEE36

    Plasmid
    #128376
    Purpose
    Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the NEO resistance marker
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    ACT1
    Available since
    April 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    128376-plasmid-map-sequence-id-292552

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  19. pPEE38

    Plasmid
    #128378
    Purpose
    Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the HYG resistance marker.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    ACT1
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    128378-plasmid-map-sequence-id-267936

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  20. pPEE32

    Plasmid
    #128372
    Purpose
    Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the NEO resistance marker
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    ACT1
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    128372-plasmid-map-sequence-id-267935

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Showing: 4841 - 4860 of 8304 results