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We narrowed to 8,781 results for: construct

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Showing: 4841 - 4860 of 8781 results

  1. single polypeptide FPX biosensor for caspase-3

    Plasmid
    #60883
    Purpose
    FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    single polypeptide FPX biosensor for caspase-3
    Tags
    HindIII site and NES sequence LALKLAGLDIGS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60883-plasmid-map-sequence-id-150659

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  2. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid
    #100936
    Purpose
    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone
    Depositor
    Ryan Lister
    Article
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    100936-plasmid-map-sequence-id-206124

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  4. LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

    Plasmid
    #100944
    Purpose
    Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag
    Depositor
    Ryan Lister
    Article
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Human, Synthetic)
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    F636A, E660A, E725A, R295A
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    100944-plasmid-map-sequence-id-206112

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  5. pSicoR Dnmt1

    Plasmid
    #12166
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF Dnmt1 shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Insert
    Dnmt1 shRNA (Dnmt1 Mouse)
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Mutation
    EGFP is expressed from this plasmid as a marker, …
    Available Since
    June 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  7. RANLS-LEHD-BNES

    Plasmid
    #60973
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Tags
    Caspase9 substrate sequence (LEHD) is after NLS s…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

    Plasmid
    #24419
    Purpose
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Expresses mCherry and Rs1 via P2A ribosomal skip sequence. Expresses tTA under EF1α promoter. Rosa26 homology arms for integration.
    Depositor
    Edward Hsiao
    Article
    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
    Inserts
    RS1 (HTR4 Human)
    tTA
    Use
    Mouse Targeting
    Tags
    FLAG and mCherry-P2A
    Mutation
    D100A
    Promoter
    EF1α and TRE, miniCMV
    Available Since
    March 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    24419-plasmid-map-sequence-id-11416

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  12. pDUAL CLDN (GFP)

    Plasmid
    #86981
    Purpose
    Lentiviral expression construct encoding Claudin-1 and GFP from separate promoters
    Depositor
    Joe Grove
    Insert
    CLDN1 (CLDN1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV/hPGK
    Available Since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86981-plasmid-map-sequence-id-173292

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  13. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

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  15. pBac[3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP]

    Plasmid
    #86456
    Purpose
    Lox responder construct with floxed (same orientation; 2xloxP, 2xlox2272) mCherry (cy) body marker and H2Av::EGFP (nu) for Cre dependent cell clone marking; (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86456-plasmid-map-sequence-id-171156

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  16. TALEN_C63

    Plasmid
    #40788
    Purpose
    Golden Gate Compatible TALEN Construct for site specific modification of genome, +63 C Terminus, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    CMV
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40788-plasmid-map-sequence-id-68904

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  17. Tet-O-FUW-mCHERRY-PURO

    Plasmid
    #251739
    Purpose
    Overexpression construct expressing mCherry-PURO and ORF-BC
    Depositor
    Andrea Califano
    Insert
    mCHERRY
    Use
    Lentiviral
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    251739-plasmid-map-sequence-id-501488

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  18. Tet-O-FUW-EGFP-PURO

    Plasmid
    #251732
    Purpose
    Overexpression construct expressing EGFP-PURO and ORF-BC
    Depositor
    Andrea Califano
    Insert
    EGFP
    Use
    Lentiviral
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    251732-plasmid-map-sequence-id-501476

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  19. pKlf4_Halo_Ty2_BactBsd

    Plasmid
    #235512
    Purpose
    C-term HALO targeting construct
    Depositor
    Brian Hendrich
    Insert
    Klf4 (Klf4 Mouse)
    Use
    Mouse Targeting
    Tags
    HALO, 2xTy
    Mutation
    None
    Promoter
    beta actin
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    235512-plasmid-map-sequence-id-474318

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  20. pSox2_Halo_Ty2_BactBsd

    Plasmid
    #235513
    Purpose
    C-term HALO targeting construct
    Depositor
    Brian Hendrich
    Insert
    Sox2 (Sox2 Mouse)
    Use
    Mouse Targeting
    Tags
    HALO, 2xTy
    Mutation
    None
    Promoter
    beta actin
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    235513-plasmid-map-sequence-id-473982

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Showing: 4841 - 4860 of 8781 results