We narrowed to 18,459 results for: gateway

Showing: 9721 - 9760 of 18459 results

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Flag-HA-JOSD3

Plasmid

#22549

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

JOSD3 (TAF1D Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pGWT35S-TIM21(1–50)-Citrine

Plasmid

#182584

Purpose

Transient expression of TIM21(1–50)-Citrine in plant cell (Mitochondria)

Depositor

Yutaka Kodama

Article

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

TIM21(1–50)

Tags

Citrine

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCZGY405

Plasmid

#172694

Purpose

Punc-25-mcherry::rab-3 Expresses Red-tagged synapses specifically in C. elegans GABAergic motor neurons

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

rab-3 (rab-3 Nematode)

Tags

Punc-25 and mcherry

Expression

Worm

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMLS253

Plasmid

#114271

Purpose

SapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)

Depositor

Erik Jorgensen

Article

Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

Insert

Cbr-Unc-119

Use

CRISPR and Cre/Lox

Mutation

*please see depositor comment below

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2J2

Plasmid

#15794

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2J2 (UBE2J2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
p3EHA

Plasmid

#67708

Purpose

3' entry HA tag

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

HA tag

Tags

HA

Promoter

None

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pIF4

Plasmid

#196069

Purpose

Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU6 promtoer in M. oryzae

Depositor

Kabin Xie

Article

Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

Insert

dCas9-Mxi1

Use

CRISPR

Promoter

MoRP27 promoter

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP2

Plasmid

#80658

Purpose

Encodes the synthetic coiled coil peptide SYNZIP2

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP2

Tags

6xHis

Expression

Bacterial

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP1

Plasmid

#80647

Purpose

Encodes the synthetic coiled coil peptide SYNZIP1

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP1

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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mpeg:lamp2-mCherry-stop; cmlc2:GFP

Plasmid

#213740

Purpose

Expresses Lamp2-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

lamp2-mCherry

Use

Zebrafish

Promoter

mpeg1.1

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCZGY2849

Plasmid

#74112

Purpose

Enhanced mutant version of membrane targeted PH-miniSOG (Q103L) entry clone for more efficient ablation of cells when expressed under desired promoter.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

miniSOG(Q103L)

Use

Gateway topo cloning

Tags

PH

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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stblHIF: pLenti-PGK-stblHIF-PGK-Puro

Plasmid

#177202

Purpose

encoding stblHIF under PGK promoter

Depositor

Markus Covert

Article

Kudo et al bioRxiv (2022)

Insert

stblHIF

Use

Lentiviral

Expression

Mammalian

Mutation

P402A/P577G/N813A

Promoter

PGK

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pACT2-Kre33

Plasmid

#99990

Purpose

Express yeast Kre33 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Kre33 (KRE33 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAC1817_pCR8-gSMN2-EX

Plasmid

#118651

Purpose

Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-EX

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving

Plasmid

#204810

Purpose

Bacterial expresison of Enterovirus A71 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

A71 2A protease

Expression

Bacterial

Available Since

Dec. 18, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pGL4_CRE-CMV-BC0502-luc2

Plasmid

#214644

Purpose

barcoded assay, cAMP/Ca2+ sensor

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

6x clustered CRE element with barcode 0502

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGWT35S-RBCS1a(1–79)-TagRFP

Plasmid

#182586

Purpose

Transient expression of RBCS1a(1–79)-TagRFP in plant cell (Plastid/Chloroplast)

Depositor

Yutaka Kodama

Article

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

RBCS1a(1–79)

Tags

TagRFP

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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Active Wnt9B-V5

Plasmid

#43821

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT9B (WNT9B Human)

Tags

V5

Expression

Mammalian

Mutation

M106T

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAC1804_pmax-dCasRx-RBM38

Plasmid

#118638

Purpose

Transient transfection; Expresses dCasRx-RBM38; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx-RBM38

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_ECFP

Plasmid

#118857

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

ECFP

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGL4_NFAT-RE-CMV-BC0547-luc2

Plasmid

#214665

Purpose

barcoded assay, Ca2+ sensor

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

6x clustered NFAT element with barcode 0547

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti PKAKTRn-Clover

Plasmid

#177200

Purpose

Lentiviral vector encoding PKAKTRnew-Clover

Depositor

Markus Covert

Article

Kudo et al Cell Syst. 2022 May 18;13(5):376-387.e8.

Insert

PKAKTRnew-mClover

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDCC5

Plasmid

#59984

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Insert

Cas9-D10A

Use

CRISPR

Tags

HA

Expression

Insect

Mutation

D10A

Promoter

hsp70Bb

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST17-hTRPT1-iso1

Plasmid

#184239

Purpose

For purification of N-terminally His-tagged TRPT1 from bacteria

Depositor

Karla Feijs

Article

Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

Insert

TRPT1 (TRPT1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pECNUS_MP02

Plasmid

#194351

Purpose

CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

pcoCas9

Expression

Plant

Promoter

RPS5A

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pPd4E-ISH

Plasmid

#61700

Purpose

Plum eIF4E ihp-RNA construct

Depositor

Lining Tian

Article

Wang et al PLoS One. 2013;8(1):e50627. doi: 10.1371

Insert

PdeIF4E

Use

RNAi

Promoter

35S

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pAC1825_pCR8-dCasRx-RBM38

Plasmid

#118662

Purpose

Gateway Donor; dCasRx-RBM38 ORF; No promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx-RBM38

Use

CRISPR

Expression

Mammalian

Promoter

No promoter

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDD364

Plasmid

#91831

Purpose

Lox511I-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Lox511I-SEC-Lox511I

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB103-Citrine

Plasmid

#79137

Purpose

Binary vectors for expression of Citrine fluorescent protein in M. polymorpha

Depositor

Yutaka Kodama

Article

Tsuboyama et al Plant Cell Physiol. 2014 Jan;55(1):229-3

Insert

Citrine

Tags

Citrine

Expression

Plant

Promoter

MpEF1a promoter

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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p6VP1

Plasmid

#24725

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

VP1 from Human Polyomavirus 6

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pDD382

Plasmid

#91830

Purpose

Lox2272-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Lox2272-SEC-Lox2272

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pME eos

Plasmid

#73792

Purpose

Photoconvertible eos in a middle entry clone

Depositor

David Raible

Article

Prendergast et al Development. 2012 Mar;139(6):1141-52. do

Insert

EOS

Use

Middle entry clone

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p7VP1

Plasmid

#24726

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

VP1 from Human Polyomavirus 7

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pAC1816_pCR8-gSMN2-DN4

Plasmid

#118649

Purpose

Transient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2U

Plasmid

#15810

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2U (UBE2U Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-jAspSnFR3-mRuby3-NLS

Plasmid

#203494

Purpose

Nuclear directed jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3-NLS

Use

Lentiviral

Tags

Nuclear localization signal and mRuby3

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-neo C-HAonly ZER1

Plasmid

#191540

Purpose

Protein expression of ZER1

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

ZER1 (ZER1 Human)

Use

Retroviral

Tags

HA

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pQLinkHD_mVenus

Plasmid

#118861

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

mVenus

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LIIalpha F 1-2 RNAi

Plasmid

#54404

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC1806_pmax-FKBP-dCasRx

Plasmid

#118639

Purpose

Transient transfection; Expresses FKBP-dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

FKBP-dCasRx

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 18,459 results for: gateway