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pRetro-XT-PAK2-CFP(puro)-WT

Plasmid

#81176

Purpose

Retroviral vector for expression of wild-type PAK2 with a CFP fusion

Depositor

Angeliki Malliri

Article

Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

Insert

p21 (RAC1) activated kinase 2 (PAK2 Human)

Use

Retroviral

Tags

CFP

Promoter

P-tight TRE

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
attB-mCh-Poly(A)

Plasmid

#65525

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 BATF3

Plasmid

#67050

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BATF3 (BATF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204342

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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LLP787_SnoopCatcher-EZH2-FL-CO

Plasmid

#211780

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-DNMT3A

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP780_SpyCatcher-KRAB-CO

Plasmid

#211773

Purpose

SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-KRAB

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-hPMCA2z/b

Plasmid

#47584

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

pMCA2z/b (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-mTcte1-PA

Plasmid

#176469

Purpose

Expression vector of mouse t-complex-associated testis expressed 1 (Tcte1) tagged with PA at C-terminus.

Depositor

Masahito Ikawa

Article

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Insert

t-complex-associated testis expressed 1 (Tcte1 Mouse)

Tags

PA

Expression

Mammalian

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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KG#205

Plasmid

#110881

Purpose

Expresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

Inserts

rab-3 promoter
pde-4d (isoform d) D448N cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Mutation

changed Asp 448 to Asn

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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TOPO tTR-KRAB-rtTA3-Luc

Plasmid

#68449

Purpose

TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

tTR-KRAB-2A-rtTA3-2A-Luciferase

Use

Luciferase; Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Human HMG-CoA lyase (HL)

Plasmid

#52433

Purpose

bacterial expression of human HMG-CoA lyase (HL)

Depositor

Henry Miziorko

Article

Montgomery et al Arch Biochem Biophys. 2011 Jul;511(1-2):

Insert

HMG CoA lyase (HMGCL Human)

Tags

His and S

Expression

Bacterial

Promoter

T7

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA5/mCherry-pBirA-HA

Plasmid

#154458

Purpose

expresses mCherry-pBirA-HA/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

mCherry-pBirA-HA

Expression

Mammalian

Promoter

CMV-F, T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo-CTRLg2

Plasmid

#139451

Purpose

Lentiviral vector with non-targeting gRNA and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-C-CARMIL2b

Plasmid

#118741

Purpose

To express CARMIL2 with GFP tag on N-terminus

Depositor

John Cooper

Article

Lanier et al Mol Biol Cell. 2015 Dec 15;26(25):4577-8

Insert

CARMIL2 (CARMIL2 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pBeast-pAhpC-RBS- LacZ

Plasmid

#190060

Purpose

LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

lacZ

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Neo1.a-AP-His

Plasmid

#71963

Purpose

Expresses the extracellular region of the Neogenin 1, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Neo1.a (Neo1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-hPPP3R1

Plasmid

#179136

Purpose

Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK-Neo-TGmiR-Luc

Plasmid

#25745

Purpose

Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pRetro-XT-PAK2-CFP(puro)-T402A

Plasmid

#81179

Purpose

Retroviral vector for expression of kinase inactive PAK2 with a CFP fusion

Depositor

Angeliki Malliri

Article

Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

Insert

p21 (RAC1) activated kinase 2 (PAK2 Human)

Use

Retroviral

Tags

CFP

Mutation

T402A

Promoter

P-tight TRE

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_huBim_Ex3

Plasmid

#85532

Purpose

Inducible expression of guide RNA (huBim_Ex3) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

hu Bim

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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WTTEM1_pSTC0-zeo

Plasmid

#202351

Purpose

Control WT TEM-1 (wild type TEM-1 beta lactamase) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

TEM-1

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-CTRLg1 (CG509)

Plasmid

#139455

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe puro H-Ras G12V E37G

Plasmid

#12275

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V E37G (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The E37G mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

Plasmid

#107324

Purpose

Expresses SpCas9 fused to NmCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to NmCas9

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL2 WT

Plasmid

#123086

Purpose

Gateway entry clone encoding human GABARAPL2 wild-type

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Gateway entry vector / entry clone

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-neo-mApg5

Plasmid

#22956

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
attB-GFP-Poly(A)‐NeoR

Plasmid

#65526

Purpose

NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[Δa10, Δ18a, 24a, Δ31a, 37b, 46]

Plasmid

#26571

Depositor

Diane Lipscombe

Article

Lin et al Neuron. 1997 Jan . 18(1):153-66.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 31a Contains e…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
KM-CashGem-Xyl2-URA3-LINEAR

Plasmid

#174838

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

TEF1p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Q769gp120-8his

Plasmid

#100929

Purpose

Mammalian expression plasmid for gp120 subunit from the Q769.d22 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q769.d22) Env

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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