We narrowed to 9,857 results for: EPO

1. pLV-GG-hUBC-EBFP2

   Plasmid

   #212314

   Purpose

   Lentiviral backbone with EBFP2 reporter

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. QUAS-jGCaMP7s-T2A-tdTomato

   Plasmid

   #200010

   Purpose

   QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

   Depositor

   Carolyn McBride

   Article

   Zhao et al Nature. 2022 May;605(7911):706-712. doi:

   Insert

   3xP3-ECFP-jGCaMP7s-td-Tomato
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Human NANOS3-P2A-mCherry knock-in targeting construct

   Plasmid

   #59721

   Purpose

   Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

   Depositor

   Jacob Hanna

   Article

   Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

   Insert

   P2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

   Plasmid

   #98974

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 3 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CAG.Flex.Twitch2B.WPRE.SV40

   Plasmid

   #100039

   Purpose

   AAV expression of FLEXed Twitch2B driven by CAG promoter. Twitch 2B is a fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch2B
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mKate2-P2A-APEX2-ML1N*2

   Plasmid

   #67665

   Purpose

   Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-ML1N*2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Twitch-3 pcDNA3

   Plasmid

   #49532

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-3
   

   Use

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-miRFP680-NES-p2A-dTomato-2xrGBD

   Plasmid

   #222638

   Purpose

   Rho reporter

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   miRFP680-NES-p2A-dTomato-2xrGBD
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol2CG2-QUASM1:GFPNLS-SV40pA

   Plasmid

   #155123

   Purpose

   Tol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QUASM1:GFPNLS
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   QUASM1-E1b

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET29a-hNABP2

    Plasmid

    #128307

    Purpose

    Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y85C- please see depositor comments below

    Promoter

    T7

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5-FLAG-Sestrin1 isoform1

    Plasmid

    #72594

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN1 (SESN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pT2-7xTcf-NLS-CNL

    Plasmid

    #65712

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, CNL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ7751 pHR Ef1α: mCherry-P2A-AcrIIA4

    Plasmid

    #125148

    Purpose

    Lentiviral vector for constitutive expression of AcrIIA4 with mCherry fluorescent reporter.

    Depositor

    Stanley Qi

    Article

    Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

    Insert

    AcrIIA4-P2A-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

    Plasmid

    #174892

    Purpose

    amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUW-tetO-NR4A3

    Plasmid

    #139818

    Purpose

    doxycycline-inducible expression of Human NR4A3 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    NR4A3 (NR4A3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S240G- please see depositor comment below

    Promoter

    TRE-mCMV

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5-FLAG-Sestrin3

    Plasmid

    #72592

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN3 (SESN3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pAGH10 - RlucFluc minus 1 HIV-1

    Plasmid

    #198224

    Purpose

    Fluorescence reporters for -1 ribosomal frameshifting in mammalian cells

    Depositor

    Isha Jain

    Article

    Baik et al Mol Cell. 2023 Mar 16;83(6):942-960.e9.

    Inserts

    Renilla luciferase
    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHJ4: LexA-VHH-Caffeine

    Plasmid

    #108242

    Purpose

    LexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    LexA DNA binding domain fusing with VHH-Caffeine antibody
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLacO1

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SnFR-gamma2

    Plasmid

    #165495

    Purpose

    Green fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.

    Depositor

    Andrew Plested

    Article

    Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

    Insert

    iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-FDIO-COX4-dAPEX2

    Plasmid

    #117178

    Purpose

    Plasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    COX4-dAPEX2
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEMI91

    Plasmid

    #74888

    Purpose

    Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information

    Depositor

    Piotr Marszalek

    Article

    Scholl et al Biomacromolecules. 2016 Jun 8.

    Insert

    Titin I27 x 9 (TTN Human)
    

    Use

    Tags

    His-tag and Strep-tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pSLQ5079_pHR_PGK_sfGFP_CoV-F1

    Plasmid

    #155303

    Purpose

    SARS-CoV-2 fluorescent reporter 1

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    Superfolder GFP fused with SARS-CoV-F1 (ORF1ab Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pEIAV-DIO-TLoop-CHR2YFP

    Plasmid

    #66798

    Purpose

    expression of channelrhodopsin-2-YFP, a light-gated cation channel that can depolarize cells via sodium influx when blue light changes its conformation, using a DIO-based TLoop EIAV vector

    Depositor

    Edward Callaway

    Article

    Cetin et al J Neurophysiol. 2014 May;111(10):2150-9.

    Insert

    Floxed Chr2-YFP
    

    Use

    Lentiviral

    Tags

    citrine

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CMVTetO7 Promoter

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. GBSGFP insulator

    Plasmid

    #121975

    Purpose

    Gli activity reporter, mouse transgenesis

    Depositor

    James Briscoe

    Article

    Balaskas et al Cell. 2012 Jan 20;148(1-2):273-84. doi:

    Insert

    GBS(long)-GFP-insulator (Gnb5 Mouse)
    

    Use

    Mouse transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

25. pEY26

    Plasmid

    #191031

    Purpose

    cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    cho-1(3.3-2.6k) (cho-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pEY39

    Plasmid

    #191043

    Purpose

    acr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-2(2.1k) (acr-2 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

    Plasmid

    #174891

    Purpose

    amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-C1-M3(T3D)471-721

    Plasmid

    #56657

    Purpose

    GFP reporter for cytoplasmic inclusions formed by the fused, C-terminal region of protein muNS from mammalian orthoreovirus Type 3 Dearing

    Depositor

    Max Nibert

    Article

    Broering et al J Virol. 2005 May;79(10):6194-206.

    Insert

    M3(T3D)421-721
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    insert encodes aa 471-721 + native stop codon of …

    Promoter

    CMV-IE

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid

    #98975

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 4 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. p221z-erVenusYFP

    Plasmid

    #71265

    Purpose

    Entry clone containing ER-localized Venus. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    VenusYFP
    

    Use

    Gateway

    Tags

    ER retention signal HDEL and signal peptide (ER)…

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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