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We narrowed to 18,759 results for: nls

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Showing: 9841 - 9880 of 18759 results

  2. (364) SB KRAB-SadCas9

    Plasmid
    #163022
    Purpose
    Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)
    Depositor
    Tonia Rex
    Publication
    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mammalian codon-optimized SadCas9
    Use
    Transposon
    Tags
    KRAB and myc NLS
    Expression
    Mammalian
    Promoter
    TET-On
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    163022-plasmid-map-sequence-id-320905

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  9. pCMV_EE-NG-nCas9

    Plasmid
    #163542
    Purpose
    Mammalian CG-to-GC base editing
    Depositor
    David Liu
    Publication
    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EE-NG-nCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…
    Available since
    Dec. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    163542-plasmid-map-sequence-id-320177

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  13. pUAS-kkv::NG

    Plasmid
    #138953
    Purpose
    Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila using Gal4
    Depositor
    Paul Adler
    Publication
    Adler et al bioRxiv 2020
    Insert
    kkv (NEWENTRY Fly)
    Use
    Uas transgene vector
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Insect
    Available since
    Nov. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138953-plasmid-map-sequence-id-274788

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  17. UAS:bloSwitch

    Plasmid
    #108355
    Purpose
    UAS driven green to red switch reporter for B3 recombinase expression
    Depositor
    Harold Burgess
    Publication
    Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.
    Insert
    UAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]
    Use
    Zebrafish expression
    Mutation
    Zebrafish codon optimized
    Promoter
    lac
    Available since
    Sept. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    108355-plasmid-map-sequence-id-208272

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  18. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

    Plasmid
    #159283
    Purpose
    A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).
    Depositor
    Rudolf Jaenisch
    Publication
    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Human INS sgRNA
    Human INS guide RNA
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159283-plasmid-map-sequence-id-311907

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  22. Plas-CRISPR_reporter

    Plasmid
    #157658
    Purpose
    CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene
    Depositor
    Frank Albert
    Publication
    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCAS9-VP64, mCherry, KanMX
    Use
    Insert storage (replicative in e. coli)
    Mutation
    N28D in mCherry- please see depositor comments be…
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    157658-plasmid-map-sequence-id-309095

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  27. Lenti Cas9/SB100

    Plasmid
    #155294
    Purpose
    Lentiviral expression of Cas9 and SB100
    Depositor
    Martin Carroll
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SB100X-P2A-spCas9
    Expression
    Mammalian
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155294-plasmid-map-sequence-id-309998

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  28. Cas9 CFP

    Plasmid
    #155278
    Purpose
    Lentiviral Cas9 expression with CFP marker
    Depositor
    Martin Carroll
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9
    Expression
    Mammalian
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155278-plasmid-map-sequence-id-309981

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  31. LCV2_BFP2

    Plasmid
    #155097
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expression
    Depositor
    Jason Moffat
    Publication
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155097-plasmid-map-sequence-id-308002

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  37. TMPRSS2 g4 + Cas9

    Plasmid
    #153018
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153018-plasmid-map-sequence-id-298698

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Showing: 9841 - 9880 of 18759 results