We narrowed to 70,796 results for: LAS

1. pRSET.HaloTag.v7

   Plasmid

   #226197

   Purpose

   bacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purification

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   HaloTag.v7
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ER-FAST

   Plasmid

   #216340

   Purpose

   For testing localization of pFAST protein to endoplasmic reticulum

   Depositor

   Alison Tebo

   Insert

   PRL_pFAST
   

   Use

   Tags

   myc for FAST protein

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. hSLC17A6/vGlutT2/pCMV6-entry

   Plasmid

   #196939

   Purpose

   Plasmid expressing an antigen targeting the VGluT2 transporter

   Depositor

   James Trimmer

   Insert

   vGlutT2/ SLC17A6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CavB4/pFlag myc-CMV

   Plasmid

   #197302

   Purpose

   Plasmid expressing an antigen targeting the Cavbeta4 calcium channel

   Depositor

   Ricardo Dolmetsch

   Insert

   Cavb4
   

   Use

   Tags

   pFLAGmyc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. cTRE-GFP

   Plasmid

   #135665

   Purpose

   Introduce Dox-inducible (TRE promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange

   Depositor

   Florian Karreth

   Insert

   GFP
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUPD2 VP16 (GB2717)

   Plasmid

   #160600

   Purpose

   Transcriptional activator

   Depositor

   Diego Orzaez

   Insert

   VP16
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. cSFFV-GFP

   Plasmid

   #135670

   Purpose

   Introduce constitutive (SFFV promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange

   Depositor

   Florian Karreth

   Insert

   GFP
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. psiCHECK2-miR-23a-3p

   Plasmid

   #135711

   Purpose

   Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-23a-3p)

   Depositor

   Florian Karreth

   Insert

   miR-23-3p reverse complement
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUPD2 GFP (GB2237)

   Plasmid

   #160591

   Purpose

   GFP coding sequence.

   Depositor

   Diego Orzaez

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MT1H

    Plasmid

    #105706

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 1h

    Depositor

    Artur Krężel

    Insert

    Metallothionein 1h (MT1H Human)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. MT4

    Plasmid

    #105711

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 4

    Depositor

    Artur Krężel

    Insert

    Metallothionein 4 (MT4 Human)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDK712

    Plasmid

    #134207

    Purpose

    Mammalian expression of EB3-tDT BMAM

    Depositor

    Iain Cheeseman

    Insert

    EB3-tDT BMAM
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. 2R2O

    Plasmid

    #51350

    Depositor

    Cheryl Arrowsmith

    Insert

    2R2O
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pLEW100v5-trunc

    Plasmid

    #33346

    Depositor

    George Cross

    Insert

    Luciferase truncated to allow cytoplasmic instead of peroxisomal (glycosomal) expression
    

    Use

    Trypanosoma brucei expression vector

    Tags

    Expression

    Mutation

    21bp of wt luciferase deleted

    Promoter

    Available since

    Dec. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-CMV-MCS-Puro

    Plasmid

    #219794

    Purpose

    Mammalian expression plasmids using piggyBac Transposon System

    Depositor

    Keiichi Nakayama

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB-hTHp-Cre-pNeo

    Plasmid

    #214122

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-CreEGFP

    Plasmid

    #165140

    Purpose

    Expression of Cre recombinase fused with EGFP in C-terminal

    Depositor

    Daekee Lee, David Threadgill

    Insert

    Cre recombinase
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-SUN2 mApple

    Plasmid

    #187679

    Purpose

    In vivo visualization of SUN2

    Depositor

    Mary Beckerle

    Insert

    SUN2 (Sun2 Mouse)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-C-Luc

    Plasmid

    #185358

    Purpose

    For mammalian expression of C-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 398-550).

    Depositor

    Ayaz Najafov

    Insert

    Firefly Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-N-Luc

    Plasmid

    #185357

    Purpose

    For mammalian expression of N-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 2-416).

    Depositor

    Ayaz Najafov

    Insert

    Firefly Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET28-iLOVf

    Plasmid

    #63723

    Purpose

    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins

    Depositor

    Brian Smith

    Type

    Empty backbone

    Use

    Tags

    TEE, iLOV, 6xHis, thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-CamkII-tTA-2A-E2-Crimson-WPRE-pA

    Plasmid

    #183708

    Purpose

    AAV designed to express tTA and-E2 Crimson from a CamKIIa promoter

    Depositor

    Jonathan Ploski

    Insert

    tTA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCAGGS-pac-T2A-iCre (v2)

    Plasmid

    #125821

    Purpose

    Puromycin resistance gene and iCre recombinase (version 2)

    Depositor

    Mario Capecchi

    Insert

    pac-T2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. QPRTA-c002

    Plasmid

    #205115

    Purpose

    QPRT protein expression

    Depositor

    Paul Brennan

    Insert

    QPRT (QPRT Human)
    

    Use

    Tags

    TEV-6His

    Expression

    Insect

    Mutation

    WT

    Promoter

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA-SUN2 EGFP

    Plasmid

    #187678

    Purpose

    In vivo visualization of SUN2

    Depositor

    Mary Beckerle

    Insert

    SUN2 (Sun2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 3S8P

    Plasmid

    #51360

    Depositor

    Cheryl Arrowsmith

    Insert

    3S8P
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

27. SDC4A-c001

    Plasmid

    #205119

    Purpose

    SDC4 protein expression

    Depositor

    Paul Brennan

    Insert

    SDC4 (SDC4 Human)
    

    Use

    Tags

    AviTag-6His and PTPRSsp

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TOPOII p48 (DM#261)

    Plasmid

    #15026

    Depositor

    Douglas Melton

    Insert

    p48 (Ptf1a Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

29. MT1A

    Plasmid

    #105700

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 1a

    Depositor

    Artur Krężel

    Insert

    Metallothionein 1a (MT1A Human)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FUW-mTie1

    Plasmid

    #119234

    Purpose

    Plasmid encodes full-length murine Tie1 gene cloned in lentiviral vector.

    Depositor

    Kari Alitalo

    Insert

    Tie1 (Tie1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    P hUbC

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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