We narrowed to 12,952 results for: sequence

1. VCDstubΔ741-752-GFP

   Plasmid

   #33123

   Depositor

   Greg Phillips

   Article

   O'Leary et al Mol Biol Cell. 2011 Sep 14.

   Insert

   Pcdh-γA3 (Pcdhga3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains a signal sequence fused to a very small …

   Promoter

   CMV

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pEG634

   Plasmid

   #40121

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Insert

   Mex-5 (aa244-468) (mex-5 Nematode)
   

   Use

   Gateway destination vector

   Tags

   Dendra2 PAFP (sequence optimized for worm express…

   Expression

   Worm

   Mutation

   contains aa244-468

   Promoter

   4.4 kb mex-5 promoter fragment

   Available Since

   Sept. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

3. mId1XGFP

   Plasmid

   #20965

   Depositor

   Robert Benezra

   Article

   Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

   Insert

   Inhibitor of DNA binding 1 (Id1 Mouse)
   

   Use

   Tags

   Stop codon 5' of the GFP coding sequence. GF…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV Ub Lex VP16 (F442A) HA (P#1711)

   Plasmid

   #14596

   Depositor

   Matija Peterlin

   Article

   Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

   Insert

   LexA
   

   Use

   Tags

   HA, Ubiquitin, and VP16 (F442A)

   Expression

   Mammalian

   Mutation

   The VP16 sequence contains an F442A mutation. The…

   Promoter

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

5. p075 pCS xTRPS1 delN delC119 (M)

   Plasmid

   #11045

   Depositor

   Ramesh Shivdasani

   Article

   Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

   Insert

   TRPS1 delN delC119 mut (trps1 Frog)
   

   Use

   Xenopus, mammalian, avian, and zebrafish

   Tags

   Expression

   Mutation

   aa806-1153 of xTRPS1. Two Cys residues in the GA…

   Promoter

   Available Since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

6. p076 pCS xTRPS1 delN delC320 (M)

   Plasmid

   #11046

   Depositor

   Ramesh Shivdasani

   Article

   Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

   Insert

   TRPS1 (trps1 Frog)
   

   Use

   Mammalian, avian, and zebrafish

   Tags

   Expression

   Mutation

   aa806-952 of xTRPS1. Two Cys residues in the GAT…

   Promoter

   Available Since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

7. p063 pCS xTRPS1 delta N (M)

   Plasmid

   #11036

   Depositor

   Ramesh Shivdasani

   Article

   Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

   Insert

   TRPS1 deltaN mut (trps1 Frog)
   

   Use

   Mammalian, avian, and zebrafish

   Tags

   Expression

   Mutation

   aa806-1153 of xTRPS1. Two Cys residues in the GA…

   Promoter

   Available Since

   Dec. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-Lyn-FRB-DMVF-181-N

   Plasmid

   #87609

   Purpose

   FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to plasma membrane.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   Lyn-FRB-DMVF181-N
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSP1E3D1

   Plasmid

   #20066

   Purpose

   Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

   Insert

   MSP1E3D1
   

   Use

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeats of …

   Promoter

   Available Since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. BFP-KDEL

    Plasmid

    #49150

    Purpose

    visualization of the ER

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    BiP ER signal sequence (HSPA5 Human)
    

    Use

    Tags

    BFP and KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLyGo-Ec-1

    Plasmid

    #163127

    Purpose

    pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Ubiquitin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIPZ shNS

    Plasmid

    #127696

    Purpose

    Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shNS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PX459-APP-KO

    Plasmid

    #176487

    Purpose

    To knockout APP gene

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    gRNA sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FLHKII-pGFPN3

    Plasmid

    #21920

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Full length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is the full length human HKII cDNA sequence …

    Promoter

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC57-LbNOX

    Plasmid

    #75285

    Purpose

    This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

    Insert

    LbNOX
    

    Use

    Tags

    C-terminal FLAG tag with linker

    Expression

    Mutation

    Human codon optimized

    Promoter

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. betaTrCP mVenus mutant reporter

    Plasmid

    #192459

    Purpose

    Fluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.

    Depositor

    Steven Cappell

    Article

    Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

    Insert

    CdC25B
    

    Use

    Lentiviral

    Tags

    SV40 NLS and mVenus

    Expression

    Mammalian

    Mutation

    aa198-338 of CDC25B. betaTrCP degron sequence (aa…

    Promoter

    EF1alpha

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mito-PAGFP

    Plasmid

    #23348

    Purpose

    Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

    Insert

    PAGFP
    

    Use

    Tags

    mito

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLyGo-Bs-1

    Plasmid

    #163137

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

    Plasmid

    #126705

    Purpose

    donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDH_SBP-EGFP-GPI

    Plasmid

    #65298

    Purpose

    synchronize trafficking of EGFP-GPI from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    GPI anchor
    

    Use

    Lentiviral

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLentiCRISPR v2-sgControl

    Plasmid

    #230080

    Purpose

    Crispr knock out control

    Depositor

    Xingguo Zhu

    Article

    Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

    Insert

    non-specific sequence control
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCW57.1-DUX4-CA

    Plasmid

    #99281

    Purpose

    All-in-one lentivector with tet-inducible DUX4 (codon sequences altered)

    Depositor

    Stephen Tapscott

    Article

    Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

    Insert

    DUX4 (codon altered) (DUX4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon altered

    Promoter

    TRE

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEGFP_IRES_tdTomato

    Plasmid

    #184046

    Purpose

    Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequence

    Depositor

    Angela Pannier

    Article

    Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

    Inserts

    Enhanced Green Fluorescent Protein
    tdTomato
    Internal Ribosome Entry Site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV-DJ8R

    Plasmid

    #213974

    Purpose

    AAV packaging vector containing AAV2-rep gene and CapDJR modified AAV-DJ8 capsid coding sequence

    Depositor

    Zhonghua Lu

    Article

    Chen et al Neurosci Bull. 2023 Jul 11. doi: 10.1007

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CAV1-deltaN-mEGFP

    Plasmid

    #27707

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1, N-terminal truncated (60-178) (CAV1 Canis lupus familiaris)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    N-terminal truncation with AA 1-59 missing. The r…

    Promoter

    Available Since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. c-Flag pcDNA3

    Plasmid

    #20011

    Purpose

    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Cyto-B

    Plasmid

    #209858

    Purpose

    Dimerization dependent fluorescent protein B fused to NES

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCP60 RS-GFP

    Plasmid

    #122173

    Purpose

    "Binary vector for Agrobacterium tumefacience-mediated transformation of plant cells resulting in constitutive expression of cytoplasmatically localized RS-GFP"

    Depositor

    Lukas Fischer

    Article

    Nocarova et al BMC Plant Biol. 2009 Apr 22;9:44. doi: 1

    Insert

    RS-GFP
    

    Use

    Binary vector for escherichia coli and agrobacter…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCRY2FL(deltaNLS)-mCherryN1

    Plasmid

    #26871

    Purpose

    Mammalian expression of cytoplasmic CRY2-mCherry

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    NLS sequences deleted

    Promoter

    CMV

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSin Helper

    Plasmid

    #127694

    Purpose

    Plasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

    Depositor

    Bryan Roth, Justin English

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    Sindbis Glycoproteins
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits