2,226 results

1. pCMV-GCaMP3fast

   Plasmid

   #74422

   Purpose

   Express fluorescent calcium indicator GCaMP3fast in mammalian cells

   Depositor

   Katalin Torok

   Article

   Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

   Insert

   GCaMP3fast
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W4Y D356A

   Promoter

   CMV

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEAK10-His-Myc-Kal7

   Plasmid

   #25454

   Depositor

   Betty Eipper

   Article

   Penzes et al J Biol Chem. 2000 Mar 3. 275(9):6395-403

   Insert

   Kal7
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 - PP1g - Flag - BirA* (AA2-140)

   Plasmid

   #86886

   Purpose

   N-terminal part of the split-BioID

   Depositor

   Mathieu Bollen

   Article

   Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

   Insert

   PP1g - FLAG - BirA (AA2-140)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   BirA Amino Acids 2-140

   Promoter

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-DIO-Magneto2.0-sNRPpA

   Plasmid

   #74307

   Purpose

   Cre-dependent expression of Magneto2.0 in mammalian cells

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)
   

   Use

   AAV and Cre/Lox

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   TRPV4: delta 760-871

   Promoter

   CMV (after Cre recombination)

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

   Plasmid

   #123614

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(R33A)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Kaede-Lysosomes-20

   Plasmid

   #57318

   Purpose

   Localization: Lysosome Membrane, Excitation: 508 / 572, Emission: 518 / 580

   Depositor

   Michael Davidson

   Insert

   LAMP1
   

   Use

   Tags

   Kaede

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

7. XE118 CAMKII-T286D-CS2+

   Plasmid

   #16736

   Depositor

   Randall Moon

   Article

   Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

   Insert

   CAMKII-T286D (Camk2a Rat)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   threonine 286 mutated to aspartate

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5-TO-PV-NES-DsRed

   Plasmid

   #16339

   Depositor

   Anton Bennett

   Article

   Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

   Insert

   PV-NES-DsRed (Pvalb Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

9. AAV-hSyn-Ribo-jGCaMP8m

   Plasmid

   #167574

   Purpose

   DEPRECATED: Please see Addgene item #169248 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Has Service

   AAV1

   Insert

   Ribo-GCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE GFP-Clta KI

    Plasmid

    #131483

    Purpose

    Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Clta Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PKC beta I WT

    Plasmid

    #16378

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC beta 1 (Prkcb Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pMK284 (Stag-3FLAG-Hygro)

    Plasmid

    #72800

    Purpose

    C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    Stag-3FLAG-Hygro
    

    Use

    CRISPR

    Tags

    Stag-3FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3 Flag p38 gamma

    Plasmid

    #20353

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 gamma (Mapk12 Rat)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. JWW-1 human chimeric antibody

    Plasmid

    #66748

    Purpose

    Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.

    Depositor

    Richard Roden

    Article

    Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

    Inserts

    JWW-1 Heavy Chain Gamma
    JWW-1 Light Chain Kappa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    First 3 amino acids of rat light chain constant r…

    Promoter

    mEF1 and rEF1

    Available since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-C1-CAMKIIalpha-T286A

    Plasmid

    #21220

    Depositor

    Tobias Meyer

    Article

    Shen et al Science. 1999 Apr 2. 284(5411):162-6.

    Insert

    CAMKIIalpha-T286A (Camk2a Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T286A (Lab plasmid KS 0003).

    Promoter

    Available since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

16. VAMP2-SNAP

    Plasmid

    #105288

    Purpose

    VAMP2 tagged with SNAP

    Depositor

    Justin Taraska

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAMP2-SNAP (Vamp2 Rat)
    

    Use

    Tags

    SNAP tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. VGluT1-SNAPtag

    Plasmid

    #107371

    Purpose

    Synaptic vesicle protein VGluT1 fused to the SNAPtag domain

    Depositor

    David Perrais

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    Vesicular Glutamate transporter 1 (Slc17a7 Rat)
    

    Use

    Tags

    SNAPtag, 104 aa before C term

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-SYN_mEGFP-CREB

    Plasmid

    #137006

    Purpose

    AAV backbone, mEGFP-CREB donor for G-CREB

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pORANGE GFP-Arpc5 KI

    Plasmid

    #131503

    Purpose

    Endogenous tagging of Arp2/3 complex subunit 5: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Arpc5, Arpc5 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Dynamin-I-K44A-6His/Flag

    Plasmid

    #32696

    Depositor

    Robert Lefkowitz

    Article

    Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

    Insert

    Dynamin I (Dnm1 Rat)
    

    Use

    Tags

    Flag and His6

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    CMV

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

21. RIP-Timer (DM#285)

    Plasmid

    #15109

    Depositor

    Douglas Melton

    Article

    Dor et al Nature. 2004 May 6. 429(6987):41-6.

    Insert

    insulin II promoter (Ins2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBK-EQR-BE3

    Plasmid

    #85172

    Purpose

    Expresses EQR-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    EQR-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AAV-SYN_mCherry-KIX-mCherry

    Plasmid

    #137007

    Purpose

    AAV backbone, mCherry-KIX acceptor for G-CREB

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    KIX domain (Crebbp Rat)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT3-EFa1-HA-Jag1

    Plasmid

    #46051

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Jag1 (Jag1 Rat)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSAD-5PSD95-EGFP-SynPhRFP

    Plasmid

    #217979

    Purpose

    G-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFP

    Depositor

    Edward Callaway

    Article

    Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

    Inserts

    5PSD95-EGFP (Dlg4 Rat)
    SynPhRFP (Syp Mouse)
    

    Use

    G-deleted rabies

    Tags

    EGFP and RFP

    Expression

    Mutation

    Promoter

    None

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. sh-SRF

    Plasmid

    #100797

    Purpose

    expression of shRNA targeting SRF

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

    Insert

    rat SRF
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV.hSyn.FLEX.iGluSnFR3.v857.IgK-NGR

    Plasmid

    #196226

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn FLEX iGluSnFR3 v857.NGR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and NGR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 HA-rnSOX9

    Plasmid

    #62972

    Purpose

    Expresses HA-tagged rat SOX9

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pMK278 (mClover-Hygro)

    Plasmid

    #72794

    Purpose

    C-terminal tagging with mClover using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mClover-Hygro
    

    Use

    CRISPR

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-Wnk1

    Plasmid

    #38780

    Depositor

    Melanie Cobb

    Article

    Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

    Insert

    Wnk1 (Wnk1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only