We narrowed to 122,981 results for: CIL
-
Plasmid#179949PurposeTagless membrane scaffold protein (MSP) for formation of nanodiscs.DepositorInsertTrpLE-M-nMSP1E3D1
TagsTrpLEExpressionBacterialMutationNative Trp residues have been mutated to Phe resi…PromoterT7Available SinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX NBP1 (P#415)
Plasmid#14658DepositorInsertATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)
ExpressionBacterialAvailable SinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-mGFP-FTL
Plasmid#192801PurposeStable expression of mGFP-FTL in mammalian cellsDepositorAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3626
Plasmid#179630PurposePacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFPDepositorAvailable SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIC292
Plasmid#51405PurposeRetroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)DepositorInsertSka2 IMAGE 4685972 (SKA2 Human)
UseRetroviralTagsEGFP, S tag, and TEV protease siteExpressionMammalianAvailable SinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Mito-CaNARi
Plasmid#64851PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.DepositorInsertCaNARi
TagsCerulean, N-terminal 30 amino acids from DAKAP1, …ExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJAF215
Plasmid#52402PurposeExpresses Flag-tagged mouse Arf1DepositorAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pISceI Dusp6:memGFP
Plasmid#32668PurposeExpresses membrane localized EGFP under the Dusp6 promoterDepositorInsertDusp6 promoter (dusp6 Zebrafish)
UseZebrafish transgenesisAvailable SinceNov. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAB088h
Plasmid#79215PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-TnCAD-F3
ExpressionBacterialPromoterPlacZ-opt (OR1+2); Ppro1Available SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal NLS
Plasmid#188396PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptide and SV40 NLSExpressionBacterialPromoterPhyper spankAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eBE-S3
Plasmid#101093PurposeExpresses eBE-S3 in mammalian cellsDepositorInserteBE-S3
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pG23A
Plasmid#102884PurposePlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.DepositorInsertCdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Endosomal Escape Control
Plasmid#186552PurposeNegative Control confirming the need for a CPP peptide in mediating internalizationDepositorInsert6H-ETA-GFP
Tags6xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization and Endosomal Escape
Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
Tags8xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB078d9
Plasmid#79207PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1+2+3); Ppro1Available SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPSP6+t
Plasmid#48149PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZ005 (AacC2c1 human codon optimized protein)
Plasmid#70171PurposeExpresses AacC2c1 human codon optimized proteinDepositorInsertAacC2c1 protein
TagsNLSAvailable SinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJR2956
Plasmid#122533PurposeExpression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2
ExpressionBacterialPromoterPnhx-2Available SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-Purg1-3HA
Plasmid#39351DepositorUseYeast genomic targetingTags3xHA and kanMX6 module - reverse orientationAvailable SinceJan. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJAF211
Plasmid#52407PurposeExpresses Flag-tagged mouse Arf6DepositorAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAB085d
Plasmid#79209PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3
ExpressionBacterialPromoterPlacZ-opt (OR1); PtetAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB088i
Plasmid#79216PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-TnCAD-F3
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB085c
Plasmid#79208PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-FL
ExpressionBacterialPromoterPlacZ-opt (OR1); PtetAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ER-BSCaM2
Plasmid#64855PurposeEndoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-BSCaM2
TagsCFP, N-terminal 27 amino acids from CYP450, and Y…ExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJR2955
Plasmid#122550PurposeExpression of a C. elegan codon optimized florescent protein (CemNeptune2.5) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemNeptune2.5
ExpressionBacterialPromoterPnhx-2Available SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2-TEV-YFP-Flag
Plasmid#41004DepositorAvailable SinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-EGFP-eDHFR(69K6)
Plasmid#178850PurposeMammalian expression of EGFP-eDHFR(69K6)DepositorInsertEGFP-eDHFR(69K6)
ExpressionMammalianPromoterCAGAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL2
Plasmid#48742PurposeExpression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +386 with respect to transcription start site at +1)DepositorInserttruncated mPer2 promoter/enhancer region (-1128 to +386)
UseLuciferaseMutationtruncated promoter region (see pGL6)Available SinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRVdG-4RFP
Plasmid#52489PurposeExpresses TagRFP-TDepositorInsertTagRFP-T
UseRabies virusPromoterCMVAvailable SinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDIV489
Plasmid#177697PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ) and TDH3p (Candida…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ER-CaNARi
Plasmid#64852PurposeEndoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-CaNARi
TagsCerulean, N-terminal 27 amino acids from CYP450, …ExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJT43_GalL_BE-P(AP)3
Plasmid#145036PurposeExpresses BE-P(AP)3 in yeast cellsDepositorInsertBE-(AP)3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT42_GalL_BE-PAPAPA
Plasmid#145035PurposeExpresses BE-PAPAPA in yeast cellsDepositorInsertBE-PAPAP
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSP6
Plasmid#48150PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLsCas13aGG
Plasmid#131012PurposeBackbone plasmid for generating CRISPR arrays for LsCas13a. Contains a GFP-dropout cassette and a direct repeat.DepositorInsertsdirect repeat of LsCas13a
GFP expression cassette
UseCRISPRExpressionBacterialAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
WZLneo-PPARγ2
Plasmid#34566DepositorAvailable SinceJan. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box
Plasmid#112257PurposeExpresses RGS component of opto-RGS2 in mammalian cellsDepositorInsertCRY2-mCherry-(GGGGS)3-hRGS2box
UseLentiviralTagsmCherryExpressionMammalianPromoterCMVAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAsCpf1GG
Plasmid#131011PurposeBackbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.DepositorInsertsdirect repeat of AsCas12a
GFP expression cassette
UseCRISPRExpressionBacterialAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-Purg1-GFP
Plasmid#39353DepositorUseYeast genomic targetingTagsGFP and kanMX6 module - reverse orientationAvailable SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJAF216
Plasmid#52405PurposeExpresses Flag-tagged mouse Arf4DepositorAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL1
Plasmid#48741PurposeExpression of luciferase driven by truncated mPer2 promoter region (bases -1128 to -141 with respect to transcription start site at +1)DepositorInserttruncated mPer2 promoter/enhancer region (-1128 to -141)
UseLuciferaseMutationtruncated promoter region (see pGL6)Available SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJR2953
Plasmid#122549PurposeExpression of a C. elegan codon optimized florescent protein (CemCardinal2) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemCardinal2
ExpressionBacterialPromoterPnhx-2Available SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1+t
Plasmid#48147PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDIV490
Plasmid#177698PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRHR2p (Debaryomyces hansenii ) and TDH3p (Candida…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3409
Plasmid#179574PurposePceh-12::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans VB-MN -specific expression of miniSOG-SL2 RFPDepositorAvailable SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL5
Plasmid#48745PurposeExpression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +2064 with respect to transcription start site at +1)DepositorInserttruncated mPer2 promoter/enhancer region (-1128 to +2064)
UseLuciferaseMutationtruncated promoter region (see pGL6)Available SinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK526
Plasmid#155387PurposePphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
PphlF-TA4 in dCas12a oscillator crRNAs
UseCRISPRExpressionBacterialMutationD917A (nuclease-deactivating)Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) HIPK3 Antisense
Plasmid#60635PurposeNegative Control for the pcDNA3.1(+) HIPK3 Sense plasmidDepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2+t
Plasmid#48148PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
QuasAr-P2A-GCaMP
Plasmid#154082PurposeIndependent expression of QuasAr and GCaMP under human synapsin 1 promoterDepositorInsertQuasAr-P2A-GCaMP6f
UseAAVExpressionMammalianMutationChanged Tyrosine 327 to AlaninePromoterhuman synapsin 1Available SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only