We narrowed to 6,165 results for: cas9 expression plasmid

1. pAAV-SMVP-Cas9C

   Plasmid

   #80931

   Purpose

   Expresses Cas9C in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lv EF1a dCas9-HA 2A Blast

   Plasmid

   #102812

   Purpose

   Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

   Depositor

   Jerry Crabtree

   Article

   Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

   Insert

   dCas9-HA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A (dCas9)

   Promoter

   EF1a

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFREE2-SpCas9-sgRNA

   Plasmid

   #179582

   Purpose

   Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFREE2-xCas9-sgRNA

   Plasmid

   #179579

   Purpose

   Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   xCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFREE2-eSpCas9-sgRNA

   Plasmid

   #179580

   Purpose

   Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   eSpCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-Puro-SMCR8

   Plasmid

   #83440

   Purpose

   Expresses Cas9 and a gRNA targeting SMCR8

   Depositor

   Shawn Ferguson

   Article

   Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

   Insert

   gRNA targeting human SMCR8 (SMCR8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI

   Plasmid

   #169097

   Purpose

   Cas9 and Citrine expression in transfected cells

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Development. 2021 Mar 9. pii: dev.193565

   Insert

   Cas9-2A-Citrine
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

   Plasmid

   #136660

   Purpose

   Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   ABE7.10
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS and hSt1Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCXLE-dCas9GFP-shP53

   Plasmid

   #102906

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9GFP-shP53
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Icam 2 SaCas9 YAP sgRNA2

    Plasmid

    #99735

    Purpose

    Expresses SaCas9 and a gRNA targeting mouse YAP

    Depositor

    Yu Huang

    Article

    Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

    Insert

    SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Icam 2 SaCas9 YAP sgRNA1

    Plasmid

    #99734

    Purpose

    Expresses SaCas9 and a gRNA targeting mouse YAP

    Depositor

    Yu Huang

    Article

    Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

    Insert

    SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNOC_dCas9_sgRNA NC

    Plasmid

    #176259

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3L

    Plasmid

    #121826

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9/Dnmt3a-3L (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223323

    Purpose

    Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Insert

    SpCas9 and sgRNA-T2 (cas9 )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Px330-EGFP-LRRK2-CRISPR/Cas9

    Plasmid

    #180437

    Purpose

    Plasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cells

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    gRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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16. BB3cH_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104912

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104910

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid

    #104908

    Purpose

    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid

    #104906

    Purpose

    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LLP791_PB_dCas9-SSSavi_BirA

    Plasmid

    #211786

    Purpose

    PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    dCas9, Spy, Snoop, aGCN4, AviTags
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    dCas9 D10A and H840A

    Promoter

    pCAGG abd pEF1a

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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