We narrowed to 13,664 results for: sequence
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Plasmid#203934PurposeUp Flank. Neutral site. Up flanking sequence of type II site-specific deoxyribonuclease AquI (FEK30_10065) in Synechococcus sp. PCC 11901.DepositorInsertaquI UP
UseSynthetic BiologyAvailable SinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.419
Plasmid#203935PurposeDown Flank. Neutral site. Down flanking sequence of type II site-specific deoxyribonuclease AquI (FEK30_10065) in Synechococcus sp. PCC 11901.DepositorInsertaquI DOWN
UseSynthetic BiologyAvailable SinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
hum sgk promoter F4R4
Plasmid#83426Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
UseLuciferaseTagsLuciferasePromotersgkAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLicC-MBP-APETx2
Plasmid#72668PurposePeriplasmic expression of APETx2 in E.coliDepositorInsertAPETx2
TagsHis tag, MBP, MalE signal sequence for periplasmi…ExpressionBacterialPromoterT7Available SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS257
Plasmid#34971PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
EK0304 CMV-TO-BFPmut-stop-Bdnf.t1 (FLP-IN)
Plasmid#191146PurposeExpression of only the trigger sequence for Bdnf.s1 (EK0301) in mammalian cellsDepositorInsertBdnf.t1
ExpressionMammalianMutationWTPromoterCMV-TOAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pSG5-ERRbeta2
Plasmid#52186PurposeExpresses human ERRbeta2 splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (ESRRB Human)
ExpressionMammalianMutationwild type sequence; codon optimized to exclude Ec…PromoterSV40Available SinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-2xMreBN-mCherry-His
Plasmid#133626PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.DepositorInsert2xMreB-mCherry-His
TagsHisExpressionBacterialAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-Pgal
Plasmid#60338PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoterDepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pV1F(Spec)
Plasmid#205086PurposeProkaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.DepositorInsertAtVIP1
ExpressionBacterialAvailable SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
ULK1-Halo HRD
Plasmid#207548PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cellsDepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag
Plasmid#231594PurposeExpress high ribosome loading circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoterDepositorInsertKozak sequence-Tornado-10xSunTag-2xALFATag
ExpressionMammalianAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry::T48-Baz::vhhGFP4
Plasmid#163928PurposeExpression of GrabFP-A-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Fragment 1 Null
Plasmid#198729PurposeGateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.DepositorInsertgRNA expression cassette in pENTR4
UseGateway-compatible entry vectorAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
vas-Cas9.874Z
Plasmid#112687PurposeExpress hSpCas9 under vasa promoterDepositorInserthSpCas9
Tags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromotervasaAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pdgfra-T2A-H2B-eGFP donor
Plasmid#113120PurposeDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequenceDepositorInsertPdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Mouse, Synthetic)
UseSynthetic BiologyAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
V2-MESA-35F-M-tTA
Plasmid#84502PurposeMESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and tTADepositorInsertV2-MESA-35F-M-tTA
UseAAVTagsFlagExpressionMammalianPromoterCMVAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-H_NES_C1
Plasmid#85061PurposeIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)DepositorInsertNES
ExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-mCherry-2xEcMTS
Plasmid#133624PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherry-2xEcMTS
TagsHisExpressionBacterialAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 CHICA (Nigg HS342)
Plasmid#41153DepositorInsertCHICA (FAM83D Human)
Tags3xMyc and PreScission SiteExpressionMammalianMutationV11LPromoterCMVAvailable SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-QF-T7-TetO-TetR_p15A
Plasmid#171661PurposeExpresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. There is no T7 terminator sequence between the QF- and TetR-containing circuits. Contains a p15A origin of replication.DepositorInsertQF-T7-TetO-TetR
ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP
Plasmid#31477DepositorInsertSFFV HA NLS Y2 T2A mTagBFP
UseLentiviralTagsHA and NLSMutationF13Y + S111Y on I-AniI = Y2 "reo" (res…Available SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAluYNF1
Plasmid#50933PurposeExpresses dimeric AluY RNA in mammalian cells.DepositorInsertAluYNF1
ExpressionMammalianPromoter7SL RNA promoter (pol III)Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-Q11
Plasmid#185781PurposeEncodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-Q11
ExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOZC-PALB2
Plasmid#133552PurposePALB2 coding sequence in pOZ-FH-C C-terminal Flag, His tagging retroviral vector.DepositorInsertPALB2 (PALB2 Human)
UseRetroviralAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-B4E
Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
Tags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is …PromoterT7Available SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-BvDODAα1
Plasmid#162528PurposeBeta vulgaris 4,5-DOPA dioxygenase alpha 1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.DepositorInsertDODAα1
UsePuc19-derivedExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-ndufs2 guide
Plasmid#107601PurposeAn entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expressionDepositorInsertndufs2 guide RNA sequences
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only