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pEMS2143

Plasmid

#104356

Purpose

AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-smCBA-EmGFP-WPRE

Use

AAV

Promoter

smCBA

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

Plasmid

#47455

Purpose

LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

NLS(alpha-imp)-CRY2PHR-NLS-VP64

Use

AAV

Tags

2A_GFP

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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SpyCas9 PE2*-3HA

Plasmid

#169854

Purpose

Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SpCas9-H840A-star-M-MLV-3HA

Tags

3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…

Expression

Mammalian

Promoter

cmv

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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myc-Atg14 (1-181)

Plasmid

#86752

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

aa 1-181

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-CDC42

Plasmid

#109531

Purpose

Muscle specific zebrafish expression of mKate2 fused to CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTarget-RFP-GFP operon

Plasmid

#192279

Purpose

RFP GFP fusion protein expressed under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

RFP GFP

Expression

Bacterial

Promoter

PlacIq

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS2031

Plasmid

#49134

Purpose

AAV plasmid with OLIG1 (Ple305) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple305-iCre

Use

AAV

Expression

Mammalian

Promoter

OLIG1

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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mEmerald-Actin-C-18

Plasmid

#53978

Purpose

Localization: Actin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Actin (ACTB Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pEMS1943

Plasmid

#82565

Purpose

High copy number plasmid containing Ple266 (S100B MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple266

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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myc-Atg14 (1-200)

Plasmid

#86750

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

Atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

aa 1-200

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-CDC42-DN

Plasmid

#109532

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-tev-std

Plasmid

#109423

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope

Tags

BAP, HA, His6, and T7

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS2271

Plasmid

#158418

Purpose

AAV plasmid with Ple341 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple341 (PCP2 Human)

Use

AAV

Promoter

Ple341

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-DysF

Plasmid

#109492

Purpose

Muscle specific zebrafish expression of mKate2 fused to dysferlin

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

DysF (dysf Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1YFP8

Plasmid

#41669

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 8 C-terminal-m…

Promoter

CMV

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mKikGR1 (mKikGR1-V69T)

Plasmid

#99227

Purpose

Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mKikGR1

Expression

Bacterial

Mutation

monomeric variant with mutation V69T (Eos notatio…

Promoter

T7

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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p6348 MSCV-CMV-Flag-HA-Brd4 1047-1362

Plasmid

#31354

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 1047-1362 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1

Plasmid

#121831

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

x-dCas9(3.7)/LSD1 (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pB-TAG-HES7

Plasmid

#130935

Purpose

PiggyBac vector for DOX-inducible human HES7-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

HES7 (HES7 Human)

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLCKO_PSMD1_sgRNA_5

Plasmid

#74181

Purpose

lentiviral vector expressing sgRNA targeting PSMD1

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMD1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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