We narrowed to 7,098 results for: 11

1. pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin

   Plasmid

   #223959

   Purpose

   Dual fluorescent reporter for histone H2B and for APC/C activity

   Depositor

   Hee Won Yang

   Article

   Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

   Insert

   H2B-iRFP670-p2a-mCerulean-Geminin (1–110) (GMNN, H2B Human)
   

   Use

   Lentiviral

   Tags

   iRFP670 and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS SARS-CoV-2 D614G Spike

   Plasmid

   #185692

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 D614G Spike

   Depositor

   Marceline Côté

   Article

   Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

   Insert

   SARS-CoV-2 D614G Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the following mutation: D614G

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   June 8, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAAV-Syn-FLEX-rc [ChrimsonR-GFP]

   Plasmid

   #84480

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   human synapsin promoter

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Tnnt2-GFP-LA

   Plasmid

   #206198

   Purpose

   Expresses GFP-tagged Lamin-A specifically in cardiomyocytes by the Tnnt2 promoter

   Depositor

   Yuxuan Guo

   Article

   Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

   Insert

   Lmna (LMNA Human)
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Tnnt2

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFB2

   Plasmid

   #195282

   Purpose

   Bicistronic DTR and Puromycin resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   DTR 2A Puro (HBEGF Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFB2A

   Plasmid

   #195283

   Purpose

   Bicistronic DTR and Hygromycin resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   DTR 2A Hygro (HBEGF Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV uN2C GFP (Ctl)

   Plasmid

   #224355

   Purpose

   Express GFP tagged upsteram ORF of NOTCH2NLC with a control size (12x) of GGC repeats

   Depositor

   Nicolas Charlet-Berguerand

   Article

   Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

   Insert

   uN2C
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Codon-optimized for expression in human, so no pu…

   Promoter

   CMV + chimeric intyron (CAG)

   Available since

   Oct. 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pDONR-BLINK2

   Plasmid

   #117075

   Purpose

   BLINK2 gene in pDONR vector for Gateway cloning system

   Depositor

   Anna Moroni

   Article

   Alberio et al Nat Methods. 2018 Nov;15(11):969-976. do

   Insert

   BLINK2
   

   Use

   Gateway cloning system

   Tags

   Expression

   Mutation

   glutamine 112 changed into aspartate (original LO…

   Promoter

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-ING2(pHD)

   Plasmid

   #21589

   Depositor

   Junying Yuan

   Article

   Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

   Insert

   GFP-ING2(pHD) (Ing2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains Ing2 plant homeodomain (PHD) amino acids…

   Promoter

   Available since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

    Plasmid

    #114072

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag
    

    Use

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    E174R, S542R, and K548R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. hU6-pegRNA-EFS-mScarlet Template

    Plasmid

    #196037

    Purpose

    The template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and EFS

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. hU6-pegRNA-EF-1α-Cre Template

    Plasmid

    #196036

    Purpose

    The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and EF-1-alpha

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1α1.1-ChrimsonR-GFP

    Plasmid

    #122063

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV2

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1a（1.1kb short version)

    Available since

    March 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-CAG-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid

    #130909

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV8

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    CAG

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Syn-FLEX-rc [Chronos-tdTomato]

    Plasmid

    #84481

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSPL3-hRPH3A_c.444Gwt

    Plasmid

    #190476

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid

    #74408

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. TdLanYFP-AURKA-Aquamarine

    Plasmid

    #228562

    Purpose

    Encodes the AURKA biosensor where LC3B is flanked by the Aquamarine/TdLanYFP donor/acceptor FRET pair and under the control of the CMV promoter.

    Depositor

    Giulia Bertolin

    Article

    Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

    Insert

    AURKA (AURKA, AURKA Human)
    

    Use

    Tags

    Aquamarine and TdLanYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)

    Plasmid

    #114086

    Purpose

    CAG promoter expression plasmid for human codon optimized LbBE1.4

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
    

    Use

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-H2B-iRFP670-p2a-YFP-Rb-IRES-blasticidin

    Plasmid

    #223962

    Purpose

    Dual fluorescent reporter for histone H2B and for exogenous Rb

    Depositor

    Hee Won Yang

    Article

    Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

    Insert

    H2B-iRFP670-p2a-YFP-Rb (RB1, H2B Human)
    

    Use

    Tags

    YFP and iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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