We narrowed to 6,088 results for: pcDNA 3.1

1. mproSAAS (21 kDa)

   Plasmid

   #231634

   Purpose

   Mammalian expression of the N-terminal domain of the mouse chaperone protein proSAAS-(1-180)

   Depositor

   Iris Lindberg

   Article

   Fortenberry et al J Biol Chem. 2002 Feb 15;277(7):5175-86.

   Insert

   Pcsk1N (Pcsk1n Mouse)
   

   Expression

   Mammalian

   Mutation

   Deletion of C-terminus (aa181-225)

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. SARS-CoV spike all5KO mutant

   Plasmid

   #231921

   Purpose

   For expression of SARS-CoV spike protein with glycan deletion at N1080, N1116, N1140, N1155, N1176 by implementing Asn-to-Gln mutation

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

   Insert

   spike all5KO (S SARS-CoV)
   

   Tags

   C9 and CD5 signal peptide

   Expression

   Mammalian

   Mutation

   N1080Q, N1116Q, N1140Q, N1155Q, N1176Q

   Promoter

   CMV

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-FCPF

   Plasmid

   #220131

   Purpose

   Expresses dCas9-FCPF in mammalian cells

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   dCas9-FCPF (cas9 Synthetic, S. pyogenes)
   

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Pt NaV1.4

   Plasmid

   #200233

   Purpose

   Expression in mammalian cells or oocytes

   Depositor

   Dan Minor

   Article

   Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

   Insert

   Phyllobates terriblis NaV1.4 – muscle channel
   

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Dt NaV1.4

   Plasmid

   #200250

   Purpose

   Expression in mammalian cells or oocytes

   Depositor

   Dan Minor

   Article

   Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

   Insert

   Dendrobates tinctorius NaV1.4 – muscle channel
   

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cas9-FCPF

   Plasmid

   #220141

   Purpose

   Expresses Cas9-FCPF in mammalian cells

   Depositor

   Xinlai Cheng

   Article

   Gama-Brambila et al JACS Au. 2021 May 12;1(6):777-785. doi:

   Insert

   Cas9-FCPF (cas9 Synthetic, S. Pyogenes)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. NSP3C-FR-2Strep

   Plasmid

   #210312

   Purpose

   Expresses the C terminal of SARS-CoV-2 NSP3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   C terminal of SARS-CoV-2 NSP3 (ORF1ab )
   

   Tags

   2Strep

   Expression

   Mammalian

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SARS-CoV-2 N-2Strep

   Plasmid

   #210376

   Purpose

   Expresses SARS-CoV-2 N protein fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-COV-2 N (N SARS-CoV-2)
   

   Tags

   2Strep

   Expression

   Mammalian

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. TLNRD1_2T_eGFP

   Plasmid

   #208883

   Purpose

   Express eGFP-TLNRD1 with the L191T and A225T mutations in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   TLNRD1 (TLNRD1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   mutations L191T and A225T

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TLNRD1_2E_eGFP

    Plasmid

    #208884

    Purpose

    Express eGFP-TLNRD1 with the K192E and R233E mutations in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    TLNRD1 (TLNRD1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mutations K192E and R233E

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. REEP6-2Strep

    Plasmid

    #210351

    Purpose

    Expresses REEP6 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    REEP6 (REEP6 Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFH2.17_GagZip-MCP-dpol-P2A-eGFP

    Plasmid

    #205532

    Purpose

    Express HIV GagZip-MCP-dpol-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag-MCP-dpol
    

    Expression

    Mammalian

    Mutation

    Nucleocapsid replaced with leucine zipper, pol fr…

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. eGFP-RAPH1-F4

    Plasmid

    #194863

    Purpose

    Express the RAPH1 fragment (1062-1250) tagged to eGFP in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acids 1062-1250

    Promoter

    CMV

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. eGFP-RAPH1-F3

    Plasmid

    #194862

    Purpose

    Express the RAPH1 fragment (868-1062) tagged to eGFP in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acids 868-1062

    Promoter

    CMV

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. eGFP-RAPH1-F2

    Plasmid

    #194861

    Purpose

    Express the RAPH1 fragment (535-868) tagged to eGFP in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acids 535-868

    Promoter

    CMV

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pClathrin-miRFP718nano

    Plasmid

    #195747

    Purpose

    Clathrin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-clathrin light chain
    

    Tags

    miRFP718nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTubulin-miRFP718nano

    Plasmid

    #195746

    Purpose

    Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-human alpha-tubulin
    

    Tags

    miRFP718nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

    Plasmid

    #194725

    Purpose

    based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D908A

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-CBS (H65R)

    Plasmid

    #182924

    Purpose

    Mammalian expression plasmid for human CBS mutant H65R

    Depositor

    Philip Eaton

    Article

    Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

    Insert

    Cystathionine Beta-Synthase (CBS Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed histidine 65 to arginine

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-mVenusC-14-HP1b Chromo W42A

    Plasmid

    #191399

    Purpose

    W42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    FLAG-mVenusC-14-HP1b Chromo W42A
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    W42A

    Promoter

    CMV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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