-
Plasmid#131122Purposereverse yTRAP, RNQ1-aTF reporting on tetR-mKate2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only
-
pSK221
Plasmid#129249Purposereverse yTRAP, tetR-responsive Neon reporterDepositorInsertpADH1(tetO2)-mNeonGreen
UseTagsExpressionYeastMutationPromoterADH1Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-DIO-mRuby2
Plasmid#104058PurposeAAV expression of Cre-dependent reporter mRuby2DepositorInsertmRuby2
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-DIO-mTurquoise2
Plasmid#104059PurposeAAV expression of Cre-dependent reporter mTurquoise2DepositorInsertmTurquoise2
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-tdTomatof
Plasmid#104060PurposeAlso known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracingDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-eYFP
Plasmid#104056PurposeAn AAV genome with tet-inducible expression of the fluorescent protein eYFPDepositorInsertEYFP
UseAAVTagsExpressionMutationPromoterTREAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGAN147
Plasmid#129202Purposeyeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backboneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAN213
Plasmid#129270PurposeControl for measuring background levels of yTRAP reporter proteinDepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromotermin pCYC1Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAN101
Plasmid#131123PurposePlasmid for overexpression of prion domains to induce prionsDepositorTypeEmpty backboneUseTagsmKate2ExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP8823
Plasmid#220901PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PC-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP8835
Plasmid#220902PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PG-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP8921
Plasmid#220903PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PC-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP8923
Plasmid#220904PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP8932
Plasmid#220906PurposeMobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)DepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-NLS-GFP
Plasmid#104061PurposeAAV expression of nuclear localized GFP with SV40 NLSDepositorHas ServiceAAV PHP.eBInsertGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-2xNLS-mTurquoise2
Plasmid#118025PurposeAn AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoterDepositorInsert2xNLS-mTurquoise2
UseAAVTagsNLSExpressionMutationPromoterhSyn1Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MBP-2xNLS-tdTomato
Plasmid#104054PurposeAn AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoterDepositorInsert2xNLS-tdTomato
UseAAVTagsNLSExpressionMutationPromoterMBPAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC-DELTA
Plasmid#53273PurposeContains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, together with the lcyE gene of Arabidopsis thaliana and thereby produces delta-carotene in E. coliDepositorInsertlcyE (LUT2 Mustard Weed)
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterTrcAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHLP700
Plasmid#203742PurposeC-terminal tagging of target proteins with AID*/3xHA degronDepositorInsertIAA17 auxin binding domain codons 71-114
UsePcr amplification template for gene taggingTags3xHAExpressionMutationPromoternoneAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHLP701
Plasmid#203743PurposeC-terminal tagging of target proteins with 3xV5-IAA17 degronDepositorInsertIAA17 auxin binding domain
UsePcr amplification template for gene taggingTags3xV5ExpressionMutationPromoternoneAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
TRE-DIO-eYFP
Plasmid#117383PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFPDepositorHas ServiceAAV1InsertEYFP
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB16 (pmyo-3::QuasAr)
Plasmid#130272PurposeExpresses the voltage sensor QuasAr in BWMs of C. elegans.DepositorInsertQuasAr
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
35S::Cel1SP-GeNL/pCAMBIA1301
Plasmid#190069PurposeExpressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplastDepositorInsertCel1 signal peptide-Green-enhanced Nano-lantern
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-2xNLS-mTurquoise2
Plasmid#104053PurposeAn AAV genome encoding expression of the nuclear localized fluorescent protein mTurquoise2 from the GFAP promoterDepositorInsert2xNLS-mTurquoise2
UseAAVTagsNLSExpressionMutationPromoterGFAPAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSK267
Plasmid#129272Purpose"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed proteinDepositorInsertNLS-VP16-ZF 43-8-NES
UseTags6xHAExpressionYeastMutationPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-eYFP-f
Plasmid#104057PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequenceDepositorInsertEYFP-f
UseAAVTagsExpressionMutationPromoterTREAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSK234
Plasmid#131149Purposedual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSK260
Plasmid#129252Purposedual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporterDepositorInsertSup35 NM yTRAP sensor reporting on mKate2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSK247
Plasmid#131148Purposedual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreenDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterpSUP35, min pCYC1Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSK226
Plasmid#129268Purposedual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporterDepositorInsertRnq1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
pAAV-CAG-DIO-EYFP
Plasmid#104052PurposeAn AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoterDepositorHas ServiceAAV PHP.V1InsertEYFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-eYFP
Plasmid#117382PurposeAn AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV2, AAV5, AAV8, and AAV9InsertEYFP
UseAAVTagsExpressionMammalianMutationPromoterhSyn1Available sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-eYFP
Plasmid#104055PurposeAn AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV2, and AAV5InsertEYFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18
Plasmid#172316PurposeCRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
MuHKII-pGFPN3
Plasmid#21922DepositorInsertMutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)
UseTagsGFPExpressionMammalianMutationThis is the full length human HKII cDNA sequence …PromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNH11 (pmyo-2::Arch(D95N)::2xMycTag)
Plasmid#130275PurposeExpresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.DepositorInsertArch(D95N)
UseTags2x Myc-tagExpressionWormMutationPromoterAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only