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Showing: 1251 - 1300 of 1457 results

  1. pAL829

    Plasmid
    #119697
    Purpose
    precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutations Y283D, G289C and V347C
    Promoter
    T5
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119697-plasmid-map-sequence-id-232493

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  11. pDN80

    Plasmid
    #61349
    Purpose
    LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.
    Depositor
    Roland Eils, Barbara Di Ventura
    Article
    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    AsLOV2 wt and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    61349-plasmid-map-sequence-id-99714

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  26. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid
    #179528
    Purpose
    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 R1333A/R1335A
    Use
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed arginines 1333 and 1335 to alanine
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179528-plasmid-map-sequence-id-351744

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  31. pJB03

    Plasmid
    #111961
    Purpose
    Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments
    Depositor
    Matthew Good
    Article
    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259
    Inserts
    mEGFP
    eDHFR
    Use
    Tags
    DHFR
    Expression
    Bacterial
    Mutation
    eDHFR;residues 2-159 and enhanced GFP with monome…
    Promoter
    Tac
    Available since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111961-plasmid-map-sequence-id-219904

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  33. hCD12

    Plasmid
    #225541
    Purpose
    Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.
    Depositor
    David Christianson
    Article
    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi
    Insert
    Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
    Use
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    225541-plasmid-map-sequence-id-446604

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Showing: 1251 - 1300 of 1457 results