We narrowed to 5,795 results for: plasmid dna

1. pcDNA3.1-MYO10-HMM-Nanotrap

   Plasmid

   #87255

   Purpose

   Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO10 (Myo10 Mouse)
   

   Use

   Tags

   GFP-nanotrap

   Expression

   Mammalian

   Mutation

   Myo10 is truncated to contain aa.1-941

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1 - HA-KLF4 FL delta K5S

   Plasmid

   #34594

   Depositor

   Michael Ruppert

   Article

   Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   deletion of K5S, corresponding to bases 1781 to 2…

   Promoter

   CMV

   Available Since

   Jan. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1/TO-myc-BirA*-cCE

   Plasmid

   #112713

   Purpose

   For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   Rngtt (Rngtt Mouse)
   

   Use

   Tags

   BirA* (BirA R118G), HIV Rev nuclear localization …

   Expression

   Mammalian

   Mutation

   Silent mutation at Arg 132 (CGT to CGC), deleted …

   Promoter

   CMV

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5-FRT/TO-LaG16-APT1

   Plasmid

   #234423

   Purpose

   Express APT1 with the anti-GFP nanobody LaG16 fused at the N terminus

   Depositor

   William Fuller

   Article

   Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

   Insert

   APT1 fused to LaG16 (LYPLA1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA5-FRT/TO-LaG16-APT2

   Plasmid

   #234422

   Purpose

   Express APT2 with the anti-GFP nanobody LaG16 fused at the N terminus

   Depositor

   William Fuller

   Article

   Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

   Insert

   APT2 fused to LaG16 (LYPLA2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-FRT/TO-APT2-LaG16

   Plasmid

   #234421

   Purpose

   Express APT2 with the anti-GFP nanobody LaG16 fused at the C terminus separated by a flexible linker

   Depositor

   William Fuller

   Article

   Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

   Insert

   APT2 fused to LaG16 (LYPLA2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5-FRT/TO-APT1-LaG16

   Plasmid

   #234420

   Purpose

   Express APT1 with the anti-GFP nanobody LaG16 fused at the C terminus separated by a flexible linker

   Depositor

   William Fuller

   Article

   Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

   Insert

   APT1 fused to LaG16 (LYPLA1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOTTC1751 - pcDNA3.1 CMV-IE V5-ER-LBD

   Plasmid

   #201818

   Purpose

   A plasmid expressing the ligand-binding domain of estrogen receptor with a V5 epitope tag from a CMV promoter

   Depositor

   Christopher Richie

   Article

   Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

   Insert

   V5-ER-LBD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-EFNB2-RBD-Fc (D62Q)

   Plasmid

   #200979

   Purpose

   Mammalian expression plasmid for soluble EFNB2 (D62Q specificity mutant) fused to IgG1 Fc

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EFNB2 (EFNB2 Human)
   

   Use

   Tags

   IgG1 Fc

   Expression

   Mammalian

   Mutation

   Soluble Receptor-Binding Domain; D62Q mutation fo…

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RGS-6xHis-LIN-29-pcDNA3.1-

    Plasmid

    #52515

    Purpose

    expresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    Lin-29 b (lin-29 Nematode)
    

    Use

    Tags

    RGS-6xHis

    Expression

    Mammalian

    Mutation

    wt (changed coding sequence in 5' (5th codon…

    Promoter

    CMV

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. (302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

    Plasmid

    #168192

    Purpose

    HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-LaG6-(EAAAK)4-OGT(4)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

    Plasmid

    #216292

    Purpose

    Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras heavy chain-GSG-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)-C-6His-mEsrra

    Plasmid

    #173152

    Purpose

    mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells

    Depositor

    Brijesh Kumar Singh

    Article

    Tripathi et al bioRxiv 2021.11.16.468891

    Insert

    ORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3 ATP5O(5’UTR)-FF

    Plasmid

    #85486

    Purpose

    Firefly luciferase under the control of ATP5O 5'UTR

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of ATP5O (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA3 ATP5O(5’UTR)-SL-FF

    Plasmid

    #85491

    Purpose

    Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of ATP5O followed by stem loop (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3 ATP5O(TISU)-FF

    Plasmid

    #85489

    Purpose

    Firefly luciferase under the control of ATP5O TISU

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    Full TISU element of ATP5O (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    2nd and 3rd codon of luciferase were modified fro…

    Promoter

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3 UQCC2(5’UTR)-FF

    Plasmid

    #85487

    Purpose

    Firefly luciferase under the control of UQCC2 5'UTR

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of UQCC2 (C6orf125 Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. (189) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4)

    Plasmid

    #160739

    Purpose

    Expresses a truncated OGT (4.5 TPRs) fused to a GFP nanobody

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nGFP-(EAAAK)4-OGT(4)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. (137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)

    Plasmid

    #160740

    Purpose

    Expresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobody

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nEPEA-(EAAAK)4-OGT(4)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. (81) pcDNA3.1-BC2-Nup62-Flag-EPEA

    Plasmid

    #168094

    Purpose

    Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    Nup62-Ubc-Flag-EPEA
    

    Use

    Tags

    BC2-Tag, EPEA-tag, and Flag-tag

    Expression

    Mammalian

    Mutation

    P229A, S283T on Nup62 native sequence

    Promoter

    T7/CMV

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.2 mir-1-1 reporter hsa-mir17~20

    Plasmid

    #46671

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir17~20 (MIR17 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. (112) pcDNA3.1-GFP-Flag-Nup62-EPEA

    Plasmid

    #168101

    Purpose

    GFP and Flag tagged Nup62 with EPEA nanobody tag

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    GFP-Flag-Nup62-EPEA
    

    Use

    Tags

    EPEA, Flag, and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

    Plasmid

    #125796

    Purpose

    for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.

    Depositor

    P. Chen

    Article

    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

    Insert

    CD40L (lose of function mutant)-mgSrtA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

    Plasmid

    #72888

    Purpose

    fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein (luminopsin) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

    Insert

    Gaussia luciferase, Channelrhodopsin-2, EYFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3_SARS-CoV-Spike[SARS-CoV-2 RBD]

    Plasmid

    #190086

    Purpose

    express chimeric SARS-CoV spike with the SARS-CoV-2 receptor binding domain

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    SARS-CoV S (S Synthetic)
    

    Use

    Tags

    Rhodopsin C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. (366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

    Plasmid

    #168189

    Purpose

    HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nUbc6E-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.2 mir-1-1 reporter dme-mir-125

    Plasmid

    #46667

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    dme-mir-125
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-CMV-LMO2 (GLuc-VChR1-EYFP)

    Plasmid

    #72889

    Purpose

    fusion protein of Gaussia luciferase, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO2) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

    Inserts

    Gaussia luciferase, Volvox channelrhodopsin 1, EYFP
    Volvox channelrhodopsin 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.2 mir-1-1 reporter dme-mir-279

    Plasmid

    #46668

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    dme-mir-279
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.2 mir-1-1 reporter dme-mir-92a

    Plasmid

    #46666

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    dme-mir-92a
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1 T21R-vhhGFP4-T2A-mCherry

    Plasmid

    #225587

    Purpose

    Mammalian expression vector for TRIM21 RING-vhhGFP4 anti-GFP degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-vhhGFP4-T2A-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

    Plasmid

    #157693

    Purpose

    mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pcDNA3.2 mir-1-1 reporter mir16-1.2

    Plasmid

    #46681

    Purpose

    Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1.2 (MIR16-1 Human, Synthetic)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1 T21R-Nb139-T2A-mCherry

    Plasmid

    #225589

    Purpose

    Mammalian expression vector for TRIM21 RING-Nb139 anti-p53 degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-Nb139-T2A-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.2 mir-1-1 reporter mir16-1.3

    Plasmid

    #46682

    Purpose

    Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1.3 (MIR16-1 Human, Synthetic)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.2 mir-1-1 reporter mir16-1.1

    Plasmid

    #46680

    Purpose

    Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1.1 (MIR16-1 Human, Synthetic)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1-GSDMD_1-270

    Plasmid

    #218229

    Purpose

    Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 1-270

    Promoter

    Available Since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pcDNA3.1-GSDMD_1-275

    Plasmid

    #218230

    Purpose

    Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 1-275

    Promoter

    Available Since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pcDNA3.1-GSDMD_29-484

    Plasmid

    #218231

    Purpose

    Gasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 29-484

    Promoter

    Available Since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pcDNA3.1-GSDMD_29-270

    Plasmid

    #218232

    Purpose

    Gasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 29-270

    Promoter

    Available Since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pcDNA5/FRT/TO-GAlphasL-RLuc8

    Plasmid

    #140981

    Purpose

    Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasL-RLuc8 (GNAS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid

    #140976

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaoA-RLuc8 (GNAO1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA5/FRT/TO-GAlphaoB-RLuc8

    Plasmid

    #140977

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaoB-Rluc8 (GNAO1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA5-FRT/TO-APT2-LaG16-3W

    Plasmid

    #234425

    Purpose

    Express APT2 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

    Depositor

    William Fuller

    Article

    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

    Insert

    APT2 fused to non-binding mutant LaG16 (LYPLA2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pcDNA5-FRT/TO-APT1-LaG16-3W

    Plasmid

    #234424

    Purpose

    Express APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

    Depositor

    William Fuller

    Article

    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

    Insert

    APT1 fused to non-binding mutant LaG16 (LYPLA1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pcDNA5/FRT/TO-GAlpha12-RLuc8

    Plasmid

    #140985

    Purpose

    Encodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha12-RLuc8 (GNA12 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

    Plasmid

    #217478

    Purpose

    Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

    Depositor

    Peng Zou

    Article

    Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

    Insert

    Nier1s
    

    Use

    Tags

    3xNLS, P2A-EGFP-3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

    Plasmid

    #217479

    Purpose

    Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

    Depositor

    Peng Zou

    Article

    Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

    Insert

    Nier1b
    

    Use

    Tags

    3xNLS, P2A-EGFP-3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1

    Plasmid

    #46676

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-133a-1 (MIR133A1 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA3.2 mir-1-1 reporter hsa-mir-122

    Plasmid

    #46673

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-122 (MIR122 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge