We narrowed to 1,221 results for: POLI

1. pT3TS-Hypa-nCas9n-A69

   Plasmid

   #125645

   Purpose

   micro-injection of poly-adenylated Hypa-nCas9n RNA

   Depositor

   Jason Berman

   Article

   Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

   Insert

   Hypa-nCas9n
   

   Use

   Rna in vitro transcription vector

   Mutation

   N692A, M694A, Q695A, H698A from wild-type Cas9

   Promoter

   T3

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK RagA(R37P)-RagC

   Plasmid

   #99706

   Purpose

   Bacterial expression of RagA(R37P)-RagC

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   R37P

   Promoter

   T7

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK RagA-RagC(L91P)

   Plasmid

   #99707

   Purpose

   Bacterial expression of RagA-RagC(L91P)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   L91P

   Promoter

   T7

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK RagA-RagC(K84T)

   Plasmid

   #99708

   Purpose

   Bacterial expression of RagA-RagC(K84T)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   K84T

   Promoter

   T7

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK RagA(T21N)-RagC

   Plasmid

   #99650

   Purpose

   Bacterial expression of RagA(T21N)-RagC

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   T21N

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK RagA-RagC(S75N)

   Plasmid

   #99699

   Purpose

   Bacterial expression of RagA-RagC(S75N)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   S75N

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pK RagA(Q66L)-RagC(Q120L)

   Plasmid

   #99702

   Purpose

   Bacterial expression of RagA(Q66L)-RagC(Q120L)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   Q120L and Q66L

   Promoter

   T7

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCMM1

   Plasmid

   #167452

   Purpose

   Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

   Depositor

   Marvin Wickens, Hugo Cesar Medina-Munoz

   Article

   Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
   

   Expression

   Yeast

   Promoter

   TEF1

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHCMM2

   Plasmid

   #167453

   Purpose

   Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

   Depositor

   Marvin Wickens, Hugo Cesar Medina-Munoz

   Article

   Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
   

   Expression

   Yeast

   Promoter

   PSEC63

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB_tetO7_sfGFP

    Plasmid

    #196616

    Purpose

    Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    Superfolder GFP
    

    Expression

    Yeast

    Promoter

    tetO7 promoter

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. c-Flag pcDNA3

    Plasmid

    #20011

    Purpose

    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACEBac1-PARP2-Flag-His-WT

    Plasmid

    #111574

    Purpose

    Expresses full length PARP2 in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP2 (PARP2 Human)
    

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. (SIM)10

    Plasmid

    #126946

    Purpose

    Bacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
    

    Expression

    Bacterial

    Promoter

    unknown

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM

    Plasmid

    #183150

    Purpose

    Expression cassettes for transcription factor induced by estradiol (Lex-ER-B112) or progesterone (ZPM)

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Inserts

    Lex-ER-B112
    Zif268 DBD - hPRLBD - MSN2AD
    

    Use

    For yeast genomic integration

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pscAluYNF1

    Plasmid

    #50934

    Purpose

    Expresses scAluY RNA in mammalian cells.

    Depositor

    Katharina Strub

    Article

    Ivanova et al Nucleic Acids Res. 2015 Mar 11;43(5):287

    Insert

    scAluYNF1
    

    Expression

    Mammalian

    Mutation

    the poly(A) linker and the right arm of the dimer…

    Promoter

    7SL RNA promoter (pol III)

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pUWR-Cad-Venus

    Plasmid

    #58328

    Purpose

    Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    Cad-Venus
    

    Expression

    Insect

    Promoter

    poly-ubiquitin

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pYFP-NEAT1m_v1-RT

    Plasmid

    #97089

    Purpose

    Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    NEAT1m_v1-RT (NEAT1 Human)
    

    Use

    CRISPR

    Tags

    EYFP

    Promoter

    CMV

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYFP-NEAT1_IS_RT

    Plasmid

    #97091

    Purpose

    Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    NEAT1_IS_RT (NEAT1 Human)
    

    Use

    CRISPR

    Promoter

    CMV

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn

    Plasmid

    #159487

    Purpose

    Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-2_PolyU(1)
    

    Expression

    Mammalian

    Promoter

    U6-27

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAMV:nAChRgamma-eGFP

    Plasmid

    #29693

    Depositor

    Henry Lester

    Article

    Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

    Insert

    GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)
    

    Tags

    AMV promotor and poly(A) tail

    Expression

    Bacterial

    Mutation

    eGFP inserted after position S380

    Available Since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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