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pLViN-iRFP670-α-cateninA+ΔβH

Plasmid

#229707

Purpose

Lentiviral expression of iRFP670-alpha-cateninA+delta-beta-H in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Mutation

amino acids 670-673, RAIM--> GSGS

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PX459-CTNNA1(α-catenin CRISPR KO)

Plasmid

#229708

Purpose

Knockout of a-catenin in mammalian cells using CRISPR-Cas9 technology

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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GST-beta catenin tag

Plasmid

#186422

Purpose

Encodes GST fused to beta catenin tag recognized by anti-beta catenin nanobody

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

GST-beta catenin tag

Use

Tags

6His and GST-beta catenin tag (PDRKAAVSHWQQRS)

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-SpyCatcher (Min)

Plasmid

#216713

Purpose

Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

Cys-SpyCatcher (Min)

Use

Tags

6xHis

Expression

Bacterial

Mutation

See Li...Howarth, J. Mol. Biol., 2014

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAB-EXPR(PYL-cat_dom_HDAC5)

Plasmid

#114401

Purpose

For PYL-HDAC5 catalytic domain expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 catalytic domain

Use

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

S220F and the deletion of M221

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET24-aCatenin(82-906)-His6

Plasmid

#203332

Purpose

Bacterial expression of wild-type alpha-catenin

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2012 May 25;287(22):18492-9

Insert

CTNNA1 (CTNNA1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 82-906

Promoter

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat(G1A)-mCherry

Plasmid

#181852

Purpose

mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Glycine residue immediately after start site in P…

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-hcvs-SpyCatcher

Plasmid

#179677

Purpose

HCVP-cleavable ER-associated SpyCatcher

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

HCVP SpyCatcher

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-Truncated-Kir3.2-StreptagII

Plasmid

#177263

Purpose

Expresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cells

Depositor

Arthur Laganowsky

Article

Qiao et al J Phys Chem Lett. 2021 Dec 30;12(51):122

Insert

G-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)

Use

Tags

StreptagII

Expression

Insect

Mutation

Residues 49-378

Promoter

polyhedrin promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-Zdk1-Vav2cat

Plasmid

#178875

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to mCherry-Zdk1

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

mCherry-Zdk1-Vav2(DH-PH-CRD) (Vav2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti

Plasmid

#138312

Purpose

Lenti expression of AcrIIA14, C-terminus

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA14, C terminus

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-3

Plasmid

#112843

Purpose

transient/stable overexpression of the C-terminal domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

C2 domain of Catulin

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-1

Plasmid

#112841

Purpose

transient/stable overexpression of the N-terminal domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

N domain of Catulin

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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KaiC CI cat- (E77Q;E78Q)

Plasmid

#41886

Depositor

Michael Rust

Article

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

KaiC CI cat -(E77Q;E78Q)

Use

Tags

Expression

Bacterial

Mutation

changed glutamic acid 77 and 78 to glutamine

Promoter

t7 promoter

Available since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
KaiC CII cat- (E318Q)

Plasmid

#41887

Depositor

Michael Rust

Article

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

Kai C CII cat (E318Q)

Use

Bl de3 21

Tags

Expression

Bacterial

Mutation

changed amino acid 318 E to Q

Promoter

t7

Available since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
XE74 TM Beta-catenin 1-9 in CS2+

Plasmid

#16832

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

TM beta-catenin 1-9 (ctnnb1.L Frog)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pLKO.1 puro shRNA beta-catenin

Plasmid

#18803

Purpose

3rd gen lentiviral vector for knocking down beta-catenin gene expression

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

b-catenin shRNA (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

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123_pAAV-ProC1-CatCh-GFP-WPRE

Plasmid

#125937

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC1

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-BX SpyCatcher003-(short)mCD80

Plasmid

#210567

Purpose

Expresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domain

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Spycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-SOD2-2A-Catalase-WPRE

Plasmid

#67635

Purpose

AAV vector expressing both SOD2 and mitochondrial targeted Catalase

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

SOD-2A-Catalase (Sod2, Cat Mouse)

Use

AAV

Tags

Expression

Mutation

Deleted the Peroxisome Targeting Signal from Cata…

Promoter

CMV

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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