We narrowed to 16,174 results for: igh

1. pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP

   Plasmid

   #226515

   Purpose

   Mamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Kühn et al bioRxiv 2024.08.28.610127

   Insert

   Cox8a(4x)-SNAP-tag2-T2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMLS716

   Plasmid

   #188888

   Purpose

   Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   floxed Cbr-unc-119 marker and Phsp16.41::Cre
   

   Use

   Tags

   Expression

   Worm

   Mutation

   C. elegans codom optimization and intron addition

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFB-LIC-Bse

   Plasmid

   #26108

   Purpose

   SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Jun 10. ():.

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   His6 - TEV

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Oct. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAID6

   Plasmid

   #136989

   Purpose

   Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

   Depositor

   Huimin Zhao

   Article

   Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

   Inserts

   dLbCpf1-VP
   dSpCas9-RD1152
   SaCas9
   Csy4
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ENO2, TDH3, TEF1, and TPI1

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. mc2 mNeon

   Plasmid

   #206218

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Brett Stringer, Simon Conn

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKin1B

   Plasmid

   #31604

   Depositor

   Anthony Brown

   Article

   Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

   Insert

   kinesin-1B (Kif5b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSYNCMV tAKARα

   Plasmid

   #119921

   Purpose

   AAV construct with tAKARα being expressed under the human synapsin promotor with WPRE enhancer.

   Depositor

   Haining Zhong

   Article

   Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

   Insert

   tAKARα
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-GFP-Dub3 mouse

   Plasmid

   #72683

   Purpose

   mammalian expression of GFP-Dub3

   Depositor

   Domenico Maiorano

   Article

   van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

   Insert

   Dub3 (Usp17le Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTripz Ctrl-IRES-mCherry

   Plasmid

   #187295

   Purpose

   Doxycyclin inducible expression of ctrl IRES-mCherry

   Depositor

   Alpha Yap

   Article

   Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

   Insert

   IRES-mCherry
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVP2A-PIF6

    Plasmid

    #73369

    Purpose

    Expresses Adeno-associated virus VP2 with PIF6 insert.

    Depositor

    Junghae Suh

    Article

    Gomez et al ACS Nano. 2015 Nov 30.

    Insert

    PIF6 (PIL2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1/Puro-CAG-VSFP-CR

    Plasmid

    #40257

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    VSFP-CR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_VASP_WH1

    Plasmid

    #109790

    Purpose

    Protein expression and purification of VASP_WH1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    VASP_WH1 (VASP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MMLV-CAG-G_oT7pol-IRES-BSD

    Plasmid

    #172369

    Purpose

    Generation of oT7pol-expressing stable cell lines, resistant to the antibiotic Blasticidin

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    oT7pol + BSD
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDGE656

    Plasmid

    #153254

    Purpose

    M2 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MMLV-CAG-SADB19_oG-IRES-Puro

    Plasmid

    #172367

    Purpose

    Generation of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    oG + Puro
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV-REPORT(PGK)-12xHRE

    Plasmid

    #172334

    Purpose

    hypoxic responsive reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAGM51559

    Plasmid

    #153226

    Purpose

    Control plasmid for knockout of TRY and CPC genes

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    Cas9, guide RNA for AP3, FAST marker
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCOLA-Gent-EM7-Erv1p

    Plasmid

    #202484

    Purpose

    Expresses sulfhydryl oxidase (Erv1p) constitutively

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    EM7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. jk56

    Plasmid

    #87009

    Purpose

    genomic Sindbis virus plasmid for expression of SYNseq components

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Inserts

    Nrx1B
    mCherry
    

    Use

    Synthetic Biology; Sindbis virus genomic plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFLAG-ZYG11A

    Plasmid

    #110551

    Purpose

    expresses a FLAG-tagged human ZYG11A protein in mamalian cells

    Depositor

    Edward Kipreos

    Article

    Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

    Insert

    ZYG11A (ZYG11A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. FU-MAP2-Gateway

    Plasmid

    #43915

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5 and non-standard His tag (HHRHHH)

    Expression

    Mammalian

    Mutation

    Promoter

    Human MAP2 promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mEmerald-MCM4-N-22

    Plasmid

    #54168

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

    Insert

    MCM4
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pDGE474

    Plasmid

    #153265

    Purpose

    M1 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDGE475

    Plasmid

    #153266

    Purpose

    M2 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSV-PGC1

    Plasmid

    #3

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Cell 1998 Mar 20;92(6):829-39.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2006

    Availability

    Academic Institutions and Nonprofits only

26. pAJM.969

    Plasmid

    #108537

    Purpose

    MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    MphRAM + EryR + PMph-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MphR: N94D

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRSET FLII12Pglu-700uDelta6

    Plasmid

    #13568

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    FLIPglu F16A Y12S-ECFP-D13R, 6aa linker
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in mglB

    Promoter

    Available since

    Feb. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pETM6-Amp-T7-roGFP

    Plasmid

    #202464

    Purpose

    Expresses redox sensitive GFP (roGFP) with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    reduction-oxidation sensitive green fluorescent protein
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRK5_tAKARα

    Plasmid

    #119913

    Purpose

    Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARα
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLifeAct_mScarlet-H_N1

    Plasmid

    #85055

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge