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Empty backbone

9,958 results

Showing: 5261 - 5280 of 9958 results

  1. pAV10.K5

    Plasmid
    #63210
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Feb. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    63210-plasmid-map-sequence-id-104911

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  2. pAV10.K4

    Plasmid
    #63212
    Purpose
    pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Feb. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    63212-plasmid-map-sequence-id-104913

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  7. TEF2pro_mCherry_YFP

    Plasmid
    #138623
    Purpose
    Dual reporter backbone plasmid for CCRA experiment
    Depositor
    Rob Mitra
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    138623-plasmid-map-sequence-id-274912

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  8. pHDR-eGFP (pXK-1647)

    Plasmid
    #208824
    Purpose
    The HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDR
    Depositor
    Kun Xu
    Article
    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208824-plasmid-map-sequence-id-417244

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  12. pPI458

    Plasmid
    #113212
    Purpose
    safe haven knock-in plasmid for the act5 locus, C-terminal mScarlet tagging
    Depositor
    Rob Kay
    Article
    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do
    Type
    Empty backbone
    Use
    Safe haven knock-in plasmid for the act5 locus, c…
    Tags
    mScarlet
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113212-plasmid-map-sequence-id-217727

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  13. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121814
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121814-plasmid-map-sequence-id-237361

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  14. pAV11K

    Plasmid
    #63184
    Purpose
    pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63184-plasmid-map-sequence-id-104886

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Showing: 5261 - 5280 of 9958 results