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pFRETtv-2in1-CN

Plasmid

#105119

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mTRQ2 and mVenus

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSG28-J23151-AraC

Plasmid

#102453

Purpose

Expresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

AraC (araC )

Expression

Bacterial

Promoter

J23151

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pHN1_lacUV5-mNG-PDT

Plasmid

#165583

Purpose

Expresses mNeonGreen and C-terminally fused PDT

Depositor

Daniel Ducat

Article

Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

Insert

mNeonGreen-PDT

Tags

Protein degradation tag

Expression

Bacterial

Promoter

Ptrc

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pUdO1c

Plasmid

#210230

Purpose

high-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pUltra-FPheKRS

Plasmid

#136631

Purpose

Genetic Incorporation 4-fluorophenyl carbamate lysine (FPheK) into proteins

Depositor

Han Xiao

Article

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

FPheKRS

Expression

Bacterial

Promoter

Tac1 promoter

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGL0_24 [pBBR1-UP ORI]

Plasmid

#198937

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1-UP ORI

Use

Synthetic Biology

Mutation

S100L (C299T)

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAct-GW-HygroR

Plasmid

#149610

Purpose

Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

Gateway cassette (ccdb + Chlor.R.)

Expression

Insect

Promoter

Act5c

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pWS1806

Plasmid

#123047

Purpose

Melatonin GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

MTNR1A [3]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS931

Plasmid

#123053

Purpose

TetR bacterial repressor

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

TetR-NLS [3a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-Ah5

Plasmid

#122572

Purpose

MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

catP/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pFRETtv-2in1-NN

Plasmid

#105125

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mTRQ2/mVenus

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pICXNHG3

Plasmid

#49021

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Promoter

AOX1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGC4593

Plasmid

#110206

Purpose

Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Inserts

TEV protease
aaRS

Expression

Bacterial

Promoter

Pbad and Ptet

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSLICER

Plasmid

#197288

Purpose

Conjugative plasmid that facilitates seamless deletions in D. radiodurans following insertion of a seamless deletion cassette. Contains an origin of transfer

Depositor

Bogumil Karas

Article

Brumwell et al Biodes Res. 2023 Mar 15;5:0009. doi: 10.

Insert

I-SceI Endonuclease

Use

Synthetic Biology; Bacterial and yeast cloning

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pWS625

Plasmid

#123046

Purpose

Adenosine GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

A2BR [3]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-FLAG-hPAX3 (#735)

Plasmid

#78336

Purpose

Doxycycline-inducible lentiviral overexpression of human PAX3 ORF with a FLAG tag

Depositor

Matthew Alexander, Louis Kunkel

Insert

PAX3 (PAX3 Human)

Use

Lentiviral

Tags

FLAG

Promoter

TRE2

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pAB168

Plasmid

#101671

Purpose

Opto-T7RNAP*(563-F1), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F1), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2108

Plasmid

#201969

Purpose

ORBIT integrating plasmid for C-terminal tagging with eGFP containing a 3C protease cleavage site.

Depositor

Gira Bhabha, Damian Ekiert

Article

Chen et al Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi

Insert

attB-3C-eGFP-4xGly-TEV-Flag-6xHis

Use

Orbit integrating plasmid

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBbE8c-RFP

Plasmid

#35269

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a-T4uvsW

Plasmid

#228201

Purpose

Expression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealase

Depositor

Stephen Benkovic

Article

Nelson et al J Biol Chem. 2007 Jan 5;282(1):407-16. d

Insert

uvsW

Tags

hexahistidine and 10 amino acid linker

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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