We narrowed to 27,904 results for: UNK

1. mEGFP-PCNA-19-SV40NLS-4

   Plasmid

   #56469

   Purpose

   Localization: Nucleus, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. K560_AviC_coBirA

   Plasmid

   #129765

   Purpose

   Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Use

   Tags

   Avi-tag GLNDIFEAQKIEWH and His6

   Expression

   Bacterial

   Mutation

   Truncation at 559

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mPA-GFP-Mito-7

   Plasmid

   #57148

   Purpose

   Localization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pLIGFP_W220F

   Plasmid

   #46772

   Purpose

   Protein overexpression with the modified LacIWF repressor

   Depositor

   Florian Hollfelder

   Article

   Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

   Insert

   LacI_W220F (lacI )
   

   Use

   Tags

   Expression

   Mutation

   Tryptophan 220 replaced by Phenylalanine

   Promoter

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAV0133

   Plasmid

   #133467

   Purpose

   Integration into fission yeast genome at ura4 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LSSmKate2-N1

   Plasmid

   #54796

   Purpose

   Localization: N1 Cloning Vector, Excitation: 460, Emission: 605

   Depositor

   Vladislav Verkhusha, Michael Davidson

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Type

   Empty backbone

   Use

   Tags

   LSSmKate2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pKB230

   Plasmid

   #170017

   Purpose

   Encodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporter

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   mNeonGreen
   Protegrin-1
   

   Use

   Synthetic Biology

   Tags

   Lpp, OmpA, and Tether

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac and PvirK

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGGD002

   Plasmid

   #48834

   Purpose

   Provides a default dummy as GreenGate C-terminal tag module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   D-Dummy
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZSm45 ndhII

   Plasmid

   #48889

   Purpose

   Lux-regulated expression of NDH-2 enzyme

   Depositor

   Jeff Hasty

   Article

   Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

   Insert

   ndhII and LuxR
   

   Use

   Synthetic Biology

   Tags

   ssrA tag (AANDENYALAA)

   Expression

   Bacterial

   Mutation

   Promoter

   pLux

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNM3732

    Plasmid

    #153086

    Purpose

    mec-4p GFP-C1 positive control for RMCE integration

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p GFP-C1 tbb-2 3'
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS406-CMV-lacI-3FLAG

    Plasmid

    #83410

    Purpose

    Expression of lacI in yeast

    Depositor

    Toshi Tsukiyama

    Article

    Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

    Insert

    lacI repressor
    

    Use

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330-Puro-ccdB

    Plasmid

    #82580

    Purpose

    A modified version of plasmid pX330 (#42230): Added are a ccdB stuffer and a Puromycin selection cassette

    Depositor

    Ivo Huijbers

    Article

    Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAM005

    Plasmid

    #170009

    Purpose

    Encodes PvirK-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    Superfolder GFP
    PhoP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTetO-1 and PvirK

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PP7 guide

    Plasmid

    #86168

    Purpose

    Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    PP7 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NLS-LucDM-GFP

    Plasmid

    #127796

    Purpose

    The expressed ORF encodes nucleus location signal with GFP and destabilized mutant Luciferase.

    Depositor

    Xiaolu Yang

    Article

    Guo et al J Vis Exp. 2016 Aug 28;(114). doi: 10.37

    Insert

    destabilized mutant (DM) Luciferase
    

    Use

    Tags

    EGFP and FLAG-NLS

    Expression

    Mammalian

    Mutation

    R188Q, R261Q in Firefly Luciferase insert

    Promoter

    CMV

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGGA006

    Plasmid

    #48816

    Purpose

    Provides the UBQ10 promoter as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    UBQ10 promoter
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. tdTomato-Farnesyl-5

    Plasmid

    #58092

    Purpose

    Localization: Membrane, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKB226

    Plasmid

    #170016

    Purpose

    Encodes LacI-controlled expression of surface-displayed defensin HNP-1 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Defensin HNP-1
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mTagBFP2-MyosinIIA-C-18

    Plasmid

    #55315

    Purpose

    Localization: Cytoskeleton, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    MyosinIIA
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

20. mTagRFP-T2-Farnesyl-5

    Plasmid

    #58037

    Purpose

    Localization: Plasma Membrane, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

21. pAM002

    Plasmid

    #170006

    Purpose

    Encodes PpmrD-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    Superfolder GFP
    PhoP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTetO-1 and PpmrD

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBXC3GH

    Plasmid

    #47070

    Purpose

    FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pL2-G(P)7G

    Plasmid

    #137100

    Purpose

    iFLinkC Linker cloning

    Depositor

    Viktor Stein

    Article

    Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

    Insert

    G(P)7G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pL2-G2

    Plasmid

    #137091

    Purpose

    iFLinkC Linker cloning

    Depositor

    Viktor Stein

    Article

    Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

    Insert

    GG
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pL2-G3

    Plasmid

    #137092

    Purpose

    iFLinkC Linker cloning

    Depositor

    Viktor Stein

    Article

    Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

    Insert

    GGG
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBS_24x-601_MA1+MA2

    Plasmid

    #114361

    Purpose

    24x Widom 601 nucleosome sequence

    Depositor

    Scot Wolfe

    Article

    Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

    Insert

    24X601-MA1_2
    

    Use

    Unspecified

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mCerulean3-Lifeact-7

    Plasmid

    #54721

    Purpose

    Localization: Actin, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Type

    Empty backbone

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mEos3.2-MAPTau-N-10

    Plasmid

    #57468

    Purpose

    Localization: Microtubules, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson

    Insert

    MAPTau (MAPT Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pMLS291

    Plasmid

    #73724

    Purpose

    SapTrap mCherry + Cbr-unc-119 donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    mCherry + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBLO44.1_dProtease

    Plasmid

    #121631

    Purpose

    CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    TEV protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken beta-actin promoter

    Available since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGBW-m4046660

    Plasmid

    #149214

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pGBW-m4046689

    Plasmid

    #149212

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pICXNH3

    Plasmid

    #49020

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCI_E01_mini-mAgrin-Myc-His-WPRE__LG1-3

    Plasmid

    #198141

    Purpose

    Express mini-Agrin in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    mini-Agrin (Agrn Mouse)
    

    Use

    Tags

    TEV-Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, beta-globin/IgG chimeric intron

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCI_E01_mini-mAgrin-Myc-His-WPRE__LG3

    Plasmid

    #198140

    Purpose

    Express mini-Agrin in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    mini-Agrin (Agrn Mouse)
    

    Use

    Tags

    TEV-Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, beta-globin/IgG chimeric intron

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGBW-m4133639

    Plasmid

    #152344

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available since

    June 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. mPA-GFP-Fibrillarin-7

    Plasmid

    #57134

    Purpose

    Localization: Nucleoli, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pBS_12x-601_MA2

    Plasmid

    #114359

    Purpose

    12x Widom 601 nucleosome sequence

    Depositor

    Scot Wolfe

    Article

    Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

    Insert

    12X601-MA2
    

    Use

    Unspecified

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSH83

    Plasmid

    #212989

    Purpose

    expresses miniSOG in C. elegans

    Depositor

    David M. Miller

    Article

    Cuentas-Condori et al Elife. 2019 Oct 4;8. pii: 47918. doi: 10

    Insert

    flp-13 promoter (flp-13 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    flp-13

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJW1234

    Plasmid

    #62367

    Purpose

    pRS305 pPGK1-BirA::LEU2

    Depositor

    Jonathan Weissman

    Article

    Jan et al Science. 2014 Nov 7;346(6210):1257521. d

    Insert

    BirA (birA )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge