We narrowed to 18,759 results for: nls
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Plasmid#195865PurposeTo express hfCas13dDepositorInserthigh fidelity (hf) version of CasRx (hfCas13d)
UseAAVMutationYes: GTGGAATACATTACCAACGTGGTGTACGTGPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-intron-hfCas13d-pA
Plasmid#195864PurposeTo express hfCas13dDepositorInserthigh fidelity (hf) version of CasRx (hfCas13d)
UseAAVMutationYes: GTGGAATACATTACCAACGTGGTGTACGTGPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHA
Plasmid#194377PurposeSplit TEV assaysDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSP-GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Human, Synthetic)
Tags2xHAExpressionMammalianPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHA
Plasmid#194381PurposeSplit TEV assaysDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSP-GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
Tags2xHAExpressionMammalianPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_DRD1-NTEV-TCS-GV-2xHA
Plasmid#194358PurposeSplit TEV assaysDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDRD1-NTEV-TCS-GV-2xHA (DRD1 Human, Synthetic)
Tags2xHAExpressionMammalianPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-GFP osTIR1
Plasmid#182514PurposeC-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.DepositorInsertAID*
TagsGFPExpressionYeastAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
114_UBI_iCre_crystGFP
Plasmid#171797PurposeTol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-)DepositorInsertoptimised iCre under the control of the Ubiquitin promoter
PromoterUbiquitinAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
iBE3 CLYBL
Plasmid#174569Purposedoxycycline inducible BE3 expression for human CLYBL locus knock-inDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE3
ExpressionMammalianPromotertet ONAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEY46
Plasmid#191050Purposeglr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb-Cas9-mCherry_cU6:6-sgRNA
Plasmid#190598PurposeThe plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
sgRNA
UseCRISPRTagsmCherry - separated by T2APromoterAedes aegypti polyubiquitin and Culex quinquefasc…Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-NCre-MBP5-WPRE
Plasmid#193918PurposeExpresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)DepositorInsertNCre-MBP5
UseAAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…ExpressionMammalianPromoterEF1aAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-NCre-MBP6-WPRE
Plasmid#193916PurposeExpresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)DepositorInsertNCre-MBP6
UseAAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…ExpressionMammalianPromoterEF1aAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pScalps eSpCas9_Zsgreen gRNA scaffold
Plasmid#188449PurposeExpression of eSpCas9 tagged with ZsgreenDepositorTypeEmpty backboneUseCRISPR and LentiviralPromoterEF1-alphaAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA
Plasmid#194723Purposebased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAsCpf1 (AAVS1 Acidaminococcus sp.)
UseCRISPRExpressionMammalianAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-scFv-VP64_Blast
Plasmid#192655Purpose3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance markerDepositorInsertscFv-VP64
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF-5Nanog_2
Plasmid#174871PurposeCRISPR vector for generating Nanog STREAMING-tag KI cellDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA for mouse Nanog (Nanog Synthetic)
UseCRISPRAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNC-KSE401
Plasmid#193435PurposeNimble cloning-compatible vector for genome editing in plantsDepositorTypeEmpty backboneExpressionPlantAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG
Plasmid#192665Purpose3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1DepositorInsertdCas9-VP64, MS2-VP64, ZsGreen1
UseCRISPR and LentiviralExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNC-BSE401
Plasmid#193436PurposeNimble cloning-compatible vector for genome editing in plantsDepositorTypeEmpty backboneExpressionPlantAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-sgTrp53-sgAtrx-EFS-Cre
Plasmid#189977PurposeExpresses Cre cDNA and sgRNAs targeting murine Trp53 and AtrxDepositorInsertsgTrp53, sgAtrx, Cre recombinase
UseAAV, Cre/Lox, and Mouse TargetingAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-dsarm(E)
Plasmid#187870PurposeGal4/UAS expression of dSarm(isoE)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdSarm isoform E (Sarm Fly)
Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-ECT2_sgRNA
Plasmid#183873PurposepX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertECT2 sgRNA spacer (ECT2 Human)
UseCRISPRExpressionMammalianPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-PR.CC9
Plasmid#192932PurposepShuttle.CC9 encoding sgRNAs targeting Trp53 and Rb1 genesDepositorAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE BI_SEPT2-14-GFP11_SEPT2-14-GFP10
Plasmid#180350Purposemammalian co-expression of human SEPT2 fused to GFP10 and of human SEPT2 fused to GFP11DepositorUseLentiviralTagsGFP10 and GFP11ExpressionMammalianPromoterbidirectional TREAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i1-14-GFP10
Plasmid#180348Purposemammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 fused to GFP11DepositorUseLentiviralTagsGFP10 and GFP11ExpressionMammalianPromoterbidirectional TREAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mammalian_SaAID
Plasmid#188653PurposeExpresses SaAID in mammalian cellsDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnSaCas9-AID-UGI
TagsUGIExpressionMammalianMutationD10A for SaCas9PromoterpCMVAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY38
Plasmid#191042Purposeunc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY17
Plasmid#191023Purposeflp-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP10B_WT-T2A-His-flag-TMEM30A variant1
Plasmid#191675Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30ADepositorAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A
Plasmid#191676Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30ADepositorUseLentiviralTags6x HIS and flagMutationD433NAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGT8162 (AATG_dttLbCas12a-i_TTCG)
Plasmid#192350PurposeL0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expressionDepositorInsertAATG_dttLbCas12a-i_TTCG
UseCRISPRMutationD156R; E925QAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-MND-HsMAN1C1-1-630-cMycFlag-hPGK-GFP
Plasmid#183505PurposeExpress GFP and MAN1C1 proteinsDepositorInsertsUseLentiviralTagsMyc-DDKExpressionMammalianPromoterMND and hPGKAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
p20StAD
Plasmid#190268PurposeConstitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.DepositorTypeEmpty backboneUseSynthetic Biology; Yeast genome-integrating vectorTagsGAL4-ADPromoterTDH3Available sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS5394-CRISPRai-MET17
Plasmid#182833PurposeSaccharomyces cerevisiae inducible CRISPRai vector - MET17 markerDepositorTypeEmpty backboneUseCRISPRAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS5395-CRISPRai-LYS2
Plasmid#182834PurposeSaccharomyces cerevisiae inducible CRISPRai vector - LYS2 markerDepositorTypeEmpty backboneUseCRISPRAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS5399-CRISPRai-ZeoR
Plasmid#182838PurposeSaccharomyces cerevisiae inducible CRISPRai vector - ZeoR markerDepositorTypeEmpty backboneUseCRISPRAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN-LexAOx4-mNeonGreen
Plasmid#192604PurposeVector for inducible mNeonGreen expression with 4x LexA operator (LexAO)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmNeonGreen
UseUnspecifiedPromoterMinimal promoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-Gluc-RMA-IRES-EGFP
Plasmid#189628PurposeExpresses Gluc-RMA and EGFP driven by the Tet Response Element (TRE). Plasmid 2 for recording Fos expression.DepositorInsertsGaussia luciferase fused to Fc
IRES-EGFP
UseAAV and LuciferaseTagsGluc and IgG1 FcExpressionMammalianPromoterIRES and TREAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY13
Plasmid#191019PurposeFluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)DepositorAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY15
Plasmid#191021Purposeflp-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only