We narrowed to 10,393 results for: plasmids 101

1. pAAV-EF1a-loxP-GRAB_DA2m-loxP

   Plasmid

   #189751

   Purpose

   Excludes sensor expression in Cre-positive neurons

   Depositor

   Yulong Li

   Article

   Dai et al Cell Rep. 2022 Aug 23;40(8):111246. doi:

   Insert

   Dopamine sensor GRAB_DA2m
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-sgRNA(MS2)-puro-barcode

   Plasmid

   #89493

   Purpose

   Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

   Depositor

   Gary Hon

   Article

   Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

   Insert

   sgRNA (with MS2 loop)
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRN3P_T3_BE4_IVT

   Plasmid

   #171762

   Purpose

   Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

   Insert

   BE4
   

   Use

   Vector for in-vitro transcription

   Promoter

   T3 promoter

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS30: pTarget(PmcCAST)

   Plasmid

   #168163

   Purpose

   Target plasmid containing PmcPSP1 and attachment site for PmcCAST.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PAM for PmcCAST + PmcPSP1
   tRNA-Val
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_PGK_SNIPR_ADAM17 site

   Plasmid

   #188349

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a ADAM17site, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_ADAM17site_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ141A2.0

   Plasmid

   #99896

   Purpose

   Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtU6

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. TC476

   Plasmid

   #122629

   Purpose

   Punc-54::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

   Depositor

   Tian Chi

   Article

   Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

   Insert

   rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
   

   Expression

   Worm

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCK255

   Plasmid

   #171155

   Purpose

   Expression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   I-SceI_sacB
   

   Expression

   Bacterial

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. APT3-PTEN (PTEN high)

   Plasmid

   #168006

   Purpose

   positive selection PACE Accessory Plasmid

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   PTEN-T3'PAP
   

   Use

   Synthetic Biology

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. APT3-VAMP4

    Plasmid

    #168004

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP4(I75-L91)-T3'PAP
    

    Use

    Synthetic Biology

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2

    Plasmid

    #79902

    Purpose

    Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Insert

    3xFLAG_Twin_Strep_EZH2
    

    Use

    CRISPR and TALEN

    Tags

    3xFLAG_Twin_Strep

    Expression

    Mammalian

    Promoter

    PGK1

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. TC513

    Plasmid

    #122616

    Purpose

    Punc-4c::SV40 NLS::rtetR::Zip(+)::SL2::mCherry

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    SV40 NLS::rtetR::Zip(+)::SL2::mCherry
    

    Expression

    Worm

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-OFF VIPER CAR (anti-CD19)-candidate (c+iii)

    Plasmid

    #192122

    Purpose

    Lentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(c+iii)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pEF1a-anti-CD19scFV_CD28_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
    

    Use

    Lentiviral

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. RFP-(SUMO FAAA)3

    Plasmid

    #130703

    Purpose

    Mammalian expression plasmid for negative control of mCherry-(SUMO)3.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    3 copies of human SUMO1 separated by (GGS)4
    

    Expression

    Mammalian

    Mutation

    FKVK sequence in each SUMO1 module changed to FAAA

    Promoter

    CMV

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgRNA1_Tet-inducible Luciferase reporter

    Plasmid

    #64161

    Purpose

    Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for Tet-inducible Luciferase reporter
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. miR-vector-RFP pCDNA

    Plasmid

    #97143

    Purpose

    for expression of miRNA and TagRFP

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Type

    Empty backbone

    Tags

    TagRFP

    Expression

    Mammalian

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. plYJP067-Ptet

    Plasmid

    #202299

    Purpose

    PTet-YFP plasmid for LPattB2 integration

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    Ptet-YFP
    

    Expression

    Bacterial

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)

    Plasmid

    #192124

    Purpose

    pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
    

    Use

    Lentiviral

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. mTubulin NLS-mCherry-LEXY

    Plasmid

    #182597

    Purpose

    Drosophila integration plasmid expressing NLS-mCherry-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    mCherry
    

    Tags

    LEXY and NLS

    Expression

    Insect

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pgRNAtet-phaZ

    Plasmid

    #169874

    Purpose

    Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    sgRNA towards phaZ in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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