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pRetroX-TRE3G_FLAG-CRTC1/MAML2_PGK-GpNLuc

Plasmid

#154265

Purpose

Dox/Tet-inducible expression of the CRTC1-MAML2 fusion oncogene in mammalian cells.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

FLAG-CRTC1/MAML2 fusion oncogene

Tags

FLAG

Expression

Mammalian

Promoter

TRE3G

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mStayGold-TOMM20

Plasmid

#227307

Purpose

Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

Plasmid

#234951

Purpose

Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1A

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pACgp67B-Her2

Plasmid

#10794

Depositor

Judy Lieberman

Article

Song et al Nat Biotechnol. 2005 Jun . 23(6):709-17.

Insert

ErbB2 ScFv (ERBB2 Human)

Tags

6His

Expression

Insect

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-sfCherry11-ß-actin

Plasmid

#70221

Purpose

Expresses sfCherry11-ß-actin in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11-actin (ACTB Human)

Tags

sfCherry11

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-COX8A

Plasmid

#227308

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of COX8A for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of COX8A (COX8A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U67

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-Halo-hMRE11(wt)

Plasmid

#208393

Purpose

To generate stable cell line expressing Halo-human MRE11(WT) by Retroviral infection.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

Human MRE11 (MRE11 Human)

Use

Luciferase and Retroviral

Expression

Mammalian

Mutation

H129N

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

Plasmid

#234952

Purpose

Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pB-CAGGS-dCas9-KRAB

Plasmid

#110822

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Irf8

Plasmid

#139838

Purpose

doxycycline-inducible expression of mouse Irf8 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Irf8 (Irf8 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA

Plasmid

#218528

Purpose

sgRNA targeting human EIF2AK4/GCN2

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK4 (EIF2AK4 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-LMNB1

Plasmid

#207770

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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shCTNNB1 # 2

Plasmid

#42544

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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shCTNNB1 # 1

Plasmid

#42543

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131004

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-AIM2

Plasmid

#73958

Purpose

Express c-Myc-tagged human AIM2 in mammalian cells

Depositor

Christian Stehlik

Article

Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

Insert

absent in melanoma 2 (AIM2) (AIM2 Human)

Tags

c-myc

Expression

Mammalian

Promoter

CMV iE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMXS-GshF-blasticidin

Plasmid

#210313

Purpose

Expresses humanized Streptococcus thermophilus GshF in mammalian cells

Depositor

Kivanc Birsoy

Article

Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

Insert

Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)

Use

Retroviral

Tags

FLAG peptide

Mutation

Codon optimized for expression in human cells

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-sfCherry11x4-ß-actin

Plasmid

#70220

Purpose

Expresses sfCherry11x4-ß-actin in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11x4-actin (ACTB Human)

Tags

sfCherry11x4

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST RBM14-V5

Plasmid

#69823

Purpose

Expresses RBM14-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

RNA-binding protein 14 (RBM14 Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Promoter

cmv

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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CPSF1

Plasmid

#155475

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPSF1 (CPSF1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro

Plasmid

#234950

Purpose

Human codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

human codon optimized Ku with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

EF1a

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pFB2D

Plasmid

#195286

Purpose

Bicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

DTR EGFP 2A Puro

Expression

Mammalian

Promoter

EFS

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Calnexin-N-14

Plasmid

#231768

Purpose

Visualization of ER in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Calnexin-N-17 (CANX Human)

Tags

mGold2s

Expression

Mammalian

Mutation

I474N

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFB2E

Plasmid

#195288

Purpose

Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK EGFP 2A Puro

Expression

Mammalian

Promoter

EFS

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Batf3

Plasmid

#139837

Purpose

doxycycline-inducible expression of mouse Batf3 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Batf3 (Batf3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Lysosome-N-20

Plasmid

#231778

Purpose

Visualization of lysosome in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Lysosome-N-20 (Lamp1 Rat)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-PH(VPS13C)

Plasmid

#232869

Purpose

Expresses the PH domain of VPS13C in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

the PH domain of VPS13C (VPS13C Human)

Tags

mCherry

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-mLin28A

Plasmid

#26357

Depositor

George Daley

Article

Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

Insert

lin-28 homolog A (Lin28a Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pmiRGLO-Zc3h12a 3’UTR

Plasmid

#222662

Purpose

Luciferase reporter vector containing mouse Zc3h12a 3'UTR

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a 3'UTR (Zc3h12a Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-MPro-eGFP-2

Plasmid

#184824

Purpose

Expresses Mpro-eGFP in mammalian cells

Depositor

Wenshe Liu

Article

Cao et al ACS Cent Sci. 2022 Feb 23;8(2):192-204.

Insert

Mpro-eGFP conjugate (M SARS-CoV-2, jellyfish Aequorea Victoria)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1alfa

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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