Addgene

157,134 results

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AAV-CAG-SPOTlight-U2GCR

Plasmid

#164819

Purpose

Reporter to measure ISR state-dependent protein synthesis

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

SPOTlight-U2GCR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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TLCV2-DelGFP

Plasmid

#133302

Purpose

EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.

Depositor

Jingshi Shen

Article

Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA

Plasmid

#140220

Purpose

Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

dLbCpf1

Use

CRISPR

Tags

miniVPR-mCherry

Expression

Mammalian

Mutation

D832A

Promoter

PGK

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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Str-Ii_VSVG-SBP-mCherry

Plasmid

#65301

Purpose

synchronize trafficking of VSVG from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ9714: pHR-PGK-SV40_NLS-dCasMINI-V4-NZF-c-Myc_NLS-mCherry-WPRE

Plasmid

#203220

Purpose

Expression of dCasMINI-V4-NZF-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCasMINI-V4-NZF-mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET16B.Pfu

Plasmid

#12509

Purpose

Bacterial expression of Pfu DNA polymerase

Depositor

Sung-Hou Kim

Article

Goldman et al Acta Crystallogr D Biol Crystallogr. 199

Insert

Pfu DNA polymerase

Use

Tags

Expression

Bacterial

Mutation

Y218H, Q462R, F533L, K559I and V683A (mutations w…

Promoter

Available since

Sept. 18, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-Axin1

Plasmid

#109370

Purpose

Transient over-expression of human Axin1

Depositor

Mariann Bienz

Article

Fiedler et al Proc Natl Acad Sci U S A. 2011 Feb 1;108

Insert

Axin1 (AXIN1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-FIRE-pHLy

Plasmid

#170775

Purpose

Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

Depositor

Aimee Kao

Article

Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

Insert

LAMP1 (LAMP1 Human)

Use

Lentiviral

Tags

mCherry and mTFP1

Expression

Mammalian

Mutation

84 nucleotide Signal Sequence switched to the beg…

Promoter

CMV

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET-19b_MMLV-RT

Plasmid

#153312

Purpose

Bacterial expression of MMLV reverse transcriptase

Depositor

Philippa Marrack, Drew Endy

Insert

MMLV Reverse Transcriptase

Use

Tags

10x His

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Lenti-Cas9-gRNA-GFP

Plasmid

#124770

Purpose

3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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lenti-tmpknot-epegRNA-puro backbone

Plasmid

#214089

Purpose

Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6, EF1a

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET-NT*-HRV3CP

Plasmid

#162795

Purpose

Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

Depositor

Gottfried Otting

Article

Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

Insert

Human rhinovirus 14 3C protease

Use

Tags

NT* solubility tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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v5 Cas9-eVLP

Plasmid

#228468

Purpose

Expresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPs

Depositor

David Liu

Article

Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

Insert

MMLVgag(C507V)–Cas9

Use

Tags

FLAG

Expression

Mammalian

Mutation

MMLVgag(C507V)

Promoter

CMV

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9 plasmid

Plasmid

#100091

Purpose

dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Type

Empty backbone

Use

CRISPR

Tags

3XFlag-NLS-dCas9-NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pUAS-NanoLuc

Plasmid

#87696

Purpose

Gal4VP16 driven expression of NanoLuc

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

NanoLuc

Use

Tags

nuclear localization signal

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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JH01

Plasmid

#73510

Purpose

GFPo_PH pCFJ150 - contains C. elegans sequence optimized GFP

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::GFPo::PH::tbb-2

Use

Tags

GFP

Expression

Mutation

Promoter

mex-5

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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ZipGFP-Casp3

Plasmid

#81241

Purpose

A ZipGFP-based Caspase-3 protease reporter T2A mCherry

Depositor

Xiaokun Shu

Article

To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

Insert

ZipGFP with caspase cleavage sequence T2A mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCH45 (multiAsCas12a-KRAB lenti)

Plasmid

#217330

Purpose

Lentiviral expression of multiAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mutation

R1226A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)

Plasmid

#204619

Purpose

Expression vector of Cascade and Cas3. Puromycin selectable.

Depositor

Akitsu Hotta

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3

Use

CRISPR

Tags

Each protein has C- and N-terminal SV40 NLS

Expression

Mutation

Promoter

EF1a

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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MP6

Plasmid

#69669

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR ugi CDA1

Use

Tags

Expression

Bacterial

Mutation

Promoter

E. coli PBAD

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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