166,027 results

1. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Plasmid

   #176534

   Purpose

   Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associated IscB (Delaware Bay metagenome)
   

   Tags

   14xHis-MBP-bdSUMO and TwinStrep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSII406

   Plasmid

   #35442

   Depositor

   Steven Haase

   Article

   Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

   Type

   Empty backbone

   Use

   Yeast integrating vector

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. BcLOV4 fusion tools screening plasmid #1

   Plasmid

   #174511

   Purpose

   Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)Cy-

   Plasmid

   #191390

   Purpose

   Donor for targeted integration of hCdt1(1-100)Cy- probe to the hROSA26 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hCdt1(1-100)Cy-
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-INU-FLAG-FAM134Bs

   Plasmid

   #128261

   Purpose

   Expresses FAM134Bs in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

   Insert

   FAM134Bs (RETREG1 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. opto-Tiam1

   Plasmid

   #174508

   Purpose

   Expresses opto-Tiam1 (BcLOV4-GGGSx2-Tiam1DH-GGGSx2-mCherry) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   opto-Tiam1-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-Miro1v4

   Plasmid

   #174111

   Purpose

   Expresses the peroxisomal variant (splice variant 4) of Miro with an N-terminal EGFP.

   Depositor

   Pietro De Camilli

   Article

   Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   Miro1 (var4, peroxisomal splice variant)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGMβ1

   Plasmid

   #216202

   Purpose

   Chromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.

   Depositor

   Yasuko Sasaki

   Article

   Iwamoto et al Biosci Microbiota Food Health. 2022;41(1

   Type

   Empty backbone

   Use

   Shuttle vector, conjugal plasmid, theta type rep…

   Expression

   Bacterial

   Promoter

   lac promoter in pGEM-Teasy,　Promoters in pAMbeta1…

   Available Since

   Jan. 21, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pBad-HisB-GRvT

   Plasmid

   #87363

   Purpose

   Expresses GRvT, a tandem heterodimer green and red fluorescent protein

   Depositor

   Robert Campbell

   Article

   Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

   Insert

   GRvT
   

   Tags

   His-Tag

   Expression

   Bacterial

   Promoter

   AraBad

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-Lck-FRB-mTurquoise2

    Plasmid

    #176130

    Purpose

    Plasma membrane localized FRB, rapamycin heterodimerization

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    FRB
    

    Use

    Lentiviral

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3 Flag MKK7b1(glu)

    Plasmid

    #14673

    Depositor

    Roger Davis

    Article

    Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

    Insert

    MKK7b1(Glu) (Map2k7 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-269Glu Thr-273Glu

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

12. BcLOV4 fusion tools screening plasmid #2

    Plasmid

    #174512

    Purpose

    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
    

    Tags

    3xFLAG and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. SSpB-iRFP-p63DH

    Plasmid

    #176120

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFRT-DestFLAGHA_HuD

    Plasmid

    #65760

    Purpose

    constitutively expresses FLAG-HA-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pMSCV2.2-IRES- GFP-Opa1

    Plasmid

    #191971

    Purpose

    Plasmid for cloning reaction, containing OPA1 coding sequence.

    Depositor

    Luca Scorrano

    Insert

    GFP-Opa1
    

    Use

    Retroviral

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-sgCdkn2a/Cre

    Plasmid

    #89644

    Purpose

    Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Cdkn2a (Cdkn2a Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. SspB-iRFP-DHPH-TIAM1

    Plasmid

    #176118

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJUMP18-Uac

    Plasmid

    #126959

    Purpose

    Universal Acceptor Plasmid. The fusion sites of the basic part generated are introduced during domestication. sfGFP reporter gene. High copy number oriV #8.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_SEPT9_i3-msfGFP

    Plasmid

    #180322

    Purpose

    mammalian expression of human SEPT9_i3 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v3 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBa.TfR-Halo

    Plasmid

    #162716

    Purpose

    Mammalian expression of human TFRC

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    TfR (TFRC Human) (TFRC Human)
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta actin

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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