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164,313 results

Showing: 521 - 540 of 164313 results

  1. pJR104

    Plasmid
    #187243
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187243-plasmid-map-sequence-id-374823

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  3. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid
    #217635
    Purpose
    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.
    Depositor
    Martin Kampmann
    Article
    Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/
    Insert
    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    EF1alpha and mU6
    Available Since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217635-plasmid-map-sequence-id-449624

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  7. pCMV-T7-EGFP (BPK1098)

    Plasmid
    #133962
    Purpose
    CMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcription
    Depositor
    Benjamin Kleinstiver
    Insert
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available Since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    133962-plasmid-map-sequence-id-265771

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  11. AiP14467 - pAAV-AiE2182m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4467)

    Plasmid
    #224043
    Purpose
    AiE2182m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_IT neurons (strong)
    Depositor
    Jonathan Ting, Allen Institute for Brain Science
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    minBG
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    224043-plasmid-map-sequence-id-449739

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  13. pCW57.1 CMYC

    Plasmid
    #164145
    Purpose
    Doxycycline inducible expression of c-MYC
    Depositor
    Richard Possemato
    Insert
    MYC (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Doxycycline inducible promoter
    Available Since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    164145-plasmid-map-sequence-id-325859

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  16. YopH (residues 164-468)

    Plasmid
    #79749
    Purpose
    This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.
    Depositor
    Nicholas Levinson, John Chodera, Markus Seeliger
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    YopH (residues 164-468)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79749-plasmid-map-sequence-id-443904

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  20. pCJ136

    Plasmid
    #162665
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162665-plasmid-map-sequence-id-321451

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Showing: 521 - 540 of 164313 results