We narrowed to 1,093 results for: Amot;

1. pQCXIP-mCherry-Halo-YAP1

   Plasmid

   #128336

   Purpose

   Retrovirus construct to express mCherry-Halo-YAP1

   Depositor

   Yutaka Hata

   Article

   Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Retroviral

   Tags

   mCherry and Halo-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-mTubb3

   Plasmid

   #87116

   Purpose

   AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-1BPNLS-Cas9-1BPNLS

   Plasmid

   #87108

   Purpose

   BPNLS-Cas9-BPNLS expression plasmid

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   1BPNLS-Cas9-1BPNLS
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL3-mFgf21pro2-luc

   Plasmid

   #101798

   Purpose

   Reporter for mouse Fgf21 promoter activity

   Depositor

   Seiichi Oyadomari

   Article

   Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

   Insert

   Fibroblast growth factor 21 promoter (Fgf21 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL3-mFgf21pro3-luc

   Plasmid

   #101799

   Purpose

   Reporter for mouse Fgf21 promoter activity

   Depositor

   Seiichi Oyadomari

   Article

   Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

   Insert

   Fibroblast growth factor 21 promoter (Fgf21 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIG-835_NY-ESO-1_TCR_FAS/MyD88

   Plasmid

   #207498

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   FAS/MyD88, NY-ESO-1_TCR (FAS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIG-832_NY-ESO-1_TCR_FAS/4-1BB

   Plasmid

   #207495

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   FAS/4-1BB, NY-ESO-1_TCR (FAS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIG-834_NY-ESO-1_TCR_FAS/IL4R

   Plasmid

   #207497

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   FAS/IL4R, NY-ESO-1_TCR (FAS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-Ai14-HITI

   Plasmid

   #87117

   Purpose

   AAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   U6-Ai14sgRNA-GFPNLS-EF1a-mCherryKASH-pA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DHC1-AID-Clover donor (Neo)

    Plasmid

    #158621

    Purpose

    Donor plasmid for tagging DHC1 with AID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR; Tagging donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIG-836_NY-ESO-1_TCR_FAS/OX40

    Plasmid

    #207499

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/OX40, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIG-833_NY-ESO-1_TCR_FAS/CD28

    Plasmid

    #207496

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/CD28, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid

    #60600

    Purpose

    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Inserts

    CRISPR Cas9 D10A
    puromycin resistance gene
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Changed Asp 10 to Ala, Codon usage optimized for …

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pInducer20_SLC38A2_wt

    Plasmid

    #156182

    Purpose

    Dox-inducible expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    TRE

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLentiCMVblast_SLC38A2_N82A

    Plasmid

    #156181

    Purpose

    CMV-driven expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    CMV

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pInducer20_SLC38A2_N82A

    Plasmid

    #156183

    Purpose

    Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    TRE

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-Ai14-luc

    Plasmid

    #87118

    Purpose

    AAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA
    

    Use

    AAV, CRISPR, and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMRX-IP-muGFP-hATG2A mLIRΔWIR

    Plasmid

    #215508

    Purpose

    Expresses GFP tagged human ATG2A which has the mutation in LC3 interacting region and lacks WIPI interacting region.

    Depositor

    Noboru Mizushima

    Article

    Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

    Insert

    Autophagy related 2A (ATG2A Human)
    

    Use

    Retroviral

    Tags

    muGFP

    Expression

    Mammalian

    Mutation

    P656R (natural variant with no functional relevan…

    Promoter

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIG-748_HA-GD2-28z_CAR_BATF

    Plasmid

    #207488

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    BATF, HA-GD2-28z_CAR (BATF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIG-805_HA-GD2-28z_CAR_BATF-RFP

    Plasmid

    #207492

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    BATF-RFP, HA-GD2-28z_CAR (BATF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIG-755_HA-GD2-28z_CAR_RFP-JUN

    Plasmid

    #207494

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    RFP-JUN, HA-GD2-28z_CAR (JUN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pIG-837_NY-ESO-1_TCR_tFAS

    Plasmid

    #207500

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    tFAS, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PCRbluntII-pHES7-STOPgRNA

    Plasmid

    #204349

    Purpose

    gRNA to target pig HES7 stop codon

    Depositor

    Li-Fang Chu

    Article

    Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

    Insert

    Pig HES7-STOP-gRNA (HES7 Sus domesticus (pig))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFC34K LgBiT TK-Neo Human collagen type IV alpha 5 chain (COL4A5)

    Plasmid

    #229770

    Purpose

    Human Col4A5 tagged at C-terminal with LgBiT to be used with C-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

    Depositor

    Hirofumi Kai

    Article

    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

    Insert

    Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)
    

    Use

    Luciferase

    Tags

    LgBiT

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFC36K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

    Plasmid

    #229769

    Purpose

    Human Col4A3 tagged at C-terminal with SmBiT to be used with C-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system

    Depositor

    Hirofumi Kai

    Article

    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

    Insert

    Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
    

    Use

    Luciferase

    Tags

    SmBiT

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFN33K LgBiT TK-neo Human collagen type IV alpha 5 chain (COL4A5)

    Plasmid

    #229768

    Purpose

    Human Col4A5 tagged at N-terminal with LgBiT to be used with N-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

    Depositor

    Hirofumi Kai

    Article

    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

    Insert

    Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)
    

    Use

    Luciferase

    Tags

    LgBiT

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

    Plasmid

    #229767

    Purpose

    Human Col4A3 tagged at N-terminal with SmBiT to be used with N-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system

    Depositor

    Hirofumi Kai

    Article

    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

    Insert

    Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
    

    Use

    Luciferase

    Tags

    SmBiT

    Expression

    Mutation

    See Depositor Comments

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-1BPNLS-Cas9-1BPNLS-2AGFP

    Plasmid

    #87109

    Purpose

    BPNLS-Cas9-BPNLS-2AGFP expression plasmid

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    1BPNLS-Cas9-1BPNLS-2A-EGFP
    

    Use

    CRISPR

    Tags

    3xFLAG (on Cas9)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-rMERTK-HITI

    Plasmid

    #87119

    Purpose

    Gene correction donor AAV for RCS rat. AAV backbone plasmid including exon 2 of rat Mertk and rMertkgRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-rMertksgRNA-Mertk(intron1-2)-nEF-GFPKASH-pA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PB-TAC-OSKM

    Plasmid

    #80481

    Purpose

    piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PB-TAC-MKOS

    Plasmid

    #80484

    Purpose

    piggyBac transposon for dox-inducible expression of the MKOS polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    MKOS (Myc, Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PB-TAC-OKMS

    Plasmid

    #80480

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OKMS (Pou5f1, Klf4, Myc, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PB-TAC-OK+9MS

    Plasmid

    #80482

    Purpose

    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OK+9MS (Pou5f1, Klf4, Myc, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [1-483]

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PB-TAC-EB-C5

    Plasmid

    #80485

    Purpose

    piggyBac transposon for dox-inducible expression of the EB-C5 polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    lin-28 homolog A (C. elegans) (Pou5f1, Sox2, Klf4, Myc, Lin28a Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMasterE

    Plasmid

    #165080

    Purpose

    Episomal expression of reprogramming factors.

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    LIN28 (LIN28A Human)
    EGFP
    HSV TK
    

    Use

    Episomal

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMasterK

    Plasmid

    #165081

    Purpose

    Episomal expression of reprogramming factors.

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    lin28 (LIN28A Human)
    EGFP
    HSV TK
    KDM4D (KDM4D Callithrix jacchus)
    GLIS1 (GLIS1 Human)
    

    Use

    Episomal

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTKA001

    Plasmid

    #164577

    Purpose

    Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp7 (ORF1ab )
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTKA002

    Plasmid

    #164578

    Purpose

    Expresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp8
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCold-TF-hPLEKHM1

    Plasmid

    #64146

    Purpose

    For protein expression in E.coli

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    PLEKHM1 (PLEKHM1 Human)
    

    Use

    Tags

    6xHis-tag and Trigger Factor (TF)

    Expression

    Bacterial

    Mutation

    Promoter

    cspA

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

40. ptfLC3

    Plasmid

    #21074

    Purpose

    Mammalian expression of rat LC3 fused to mRFP and EGFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kimura et al Autophagy. 2007;3(5):452-60.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge