We narrowed to 8,771 results for: ef1

1. pAAV-EF1a-ArgiNLS-AausFP1

   Plasmid

   #220595

   Purpose

   Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   AausFP1
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLX-EF1a_NKX2-1_DelNTerm_160-371AA

   Plasmid

   #221487

   Purpose

   NKX2-1 N-terminal truncation mutation lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Deletion of 1-159AA N-terminal domain

   Promoter

   EF1a

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLX-EF1a_NKX2-1_DelCTerm_1-223AA

   Plasmid

   #221488

   Purpose

   NKX2-1 C-terminal truncation mutation lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Deletion of 224-371AA C-terminal domain

   Promoter

   EF1a

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLX-EF1a_NKX2-1_HD_160-223AA

   Plasmid

   #221489

   Purpose

   NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Deletion of 1-159AA N-terminal and 224-371AA C-te…

   Promoter

   EF1a

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EF1a-hP53DD (TET+CTD)

   Plasmid

   #214082

   Purpose

   Mammalian expression tetramerization domain (TET) and C-terminal negative regulatory domain (CTD) of human TP53

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   hP53DD (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EF1a-SRRD-V5-APEX2 (in pLEX_307)

   Plasmid

   #214921

   Purpose

   constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   SRRD (SRRD Human)
   

   Use

   Lentiviral

   Tags

   V5-APEX2

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EF1a-SRRD-noTag (in pLEX_307)

   Plasmid

   #214919

   Purpose

   constitutive expression of SRRD with no tag - used as control for SRRD-HA expression

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   SRRD (SRRD Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSmart-EF1alpha-NlaCas7-p65

   Plasmid

   #207394

   Purpose

   Expresses NlaCas7-p65 activator fusion in mammalian cells

   Depositor

   Yan Zhang

   Article

   Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

   Insert

   EF1a-NlaCas7-p65-bGH polyA
   

   Use

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSmart-EF1alpha-AcrIC8-NLS

   Plasmid

   #207395

   Purpose

   Expresses AcrIC8 with NLS in mammalian cells

   Depositor

   Yan Zhang

   Article

   Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

   Insert

   EF1a-AcrIC8-bGH polyA
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSmart-EF1alpha-AcrIE2-NLS

    Plasmid

    #207396

    Purpose

    Expresses AcrIE2 with NLS in mammalian cells

    Depositor

    Yan Zhang

    Article

    Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

    Insert

    EF1a-AcrIE2-bGH polyA
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSmart-EF1alpha-AcrIC9-NLS

    Plasmid

    #207397

    Purpose

    Expresses AcrIC9 with NLS in mammalian cells

    Depositor

    Yan Zhang

    Article

    Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

    Insert

    EF1a-AcrIC9-bGH polyA
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOttc556 - pAAV EF1a iCre

    Plasmid

    #210416

    Purpose

    AAV viral vector packaging plasmid that expresses "improved Cre recombinase"

    Depositor

    Brandon Harvey, Christopher Richie

    Insert

    iCre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti7-3_EF1ap-mRuby2_hU6p_sgSTK4i_#1 (BlasR)

    Plasmid

    #209776

    Purpose

    CRISPRi for STK4

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    Nluc, sgSTK4i, mRuby2 (STK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A

    Plasmid

    #204622

    Purpose

    Single-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable.

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Insert

    mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPV-C1-crRNA-cloning-EF1a-BA

    Plasmid

    #204623

    Purpose

    piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV-REPORT(EF1a no GFP)

    Plasmid

    #172339

    Purpose

    mnucTomato

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-REPORT(EF1a)TetR

    Plasmid

    #172352

    Purpose

    Tet Repressor reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    TetR
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV/EF1a::DIO-iLMO2

    Plasmid

    #129482

    Purpose

    AAV2 transfer plasmid for Cre-dependent expression of iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

    Depositor

    Robert Gross

    Article

    Jaiswal et al J Neurosci Res. 2017 Aug 3. doi: 10.1002

    Insert

    iLMO2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Ef1a-DIO-Aion-citrine

    Plasmid

    #192571

    Purpose

    AAV-vector for optogenetic long-term silencing of Cre-positive neurons

    Depositor

    Simon Wiegert

    Insert

    Aion
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    Ef1-alpha

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ef1a-DIO-somAion-citrine

    Plasmid

    #192573

    Purpose

    AAV-vector for optogenetic long-term silencing of Cre-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somAion
    

    Use

    AAV

    Tags

    Citrine - soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    Ef1-alpha

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKLV2-EF1a-GFP RanSen

    Plasmid

    #184697

    Purpose

    Lentiviral transfer plasmid for expression of GFP RanSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP RanSen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKLV2-EF1a-ArgSen (-)NLS

    Plasmid

    #184689

    Purpose

    Lentiviral transfer plasmid for expression of ArgSen (-)NLS

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    ArgSen (-)NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLV-EF1a-ArgSen (-)NLS

    Plasmid

    #184698

    Purpose

    Lentiviral transfer plasmid for expression of ArgSen (-)NLS

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    ArgSen (-)NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAID1.2-EF1a-NmClover3-hsLem2

    Plasmid

    #179524

    Purpose

    Express OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the control of EF1a promoter

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    LEMD2 (LEMD2 Human)
    

    Use

    Tags

    mClover3-mAID

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEF1α-mouse-CHMP3(147)-HA

    Plasmid

    #154177

    Purpose

    expresses mouse CHMP3(147) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3 (Chmp3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal truncation of CHMP3 at amino acid 147

    Promoter

    EF1alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV x EF1a_EKAREN4(TA)-ires-blast

    Plasmid

    #167831

    Purpose

    Control sensor for EKAREN4

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPD1024 EF1alpha TetON3G in TU5

    Plasmid

    #139261

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV5

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJM469 EF1alpha TetON3G in TU4

    Plasmid

    #139260

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV4

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJM422 EF1alpha TetON3G in TU3

    Plasmid

    #139259

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV3

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Clim EF1 MCH (CEM)

    Plasmid

    #104444

    Purpose

    Expresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.

    Depositor

    Inaki Ruiz-Trillo, Elena Casacuberta

    Insert

    mCherry
    

    Use

    Expression in corallochytrium (single-celled euka…

    Tags

    Expression

    Mutation

    Promoter

    EF1

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRS416-PTEF1-MCP-3P-ECM33_intron

    Plasmid

    #127601

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron with MS2 hairpins inserted near 3′ end of intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRS416-PTEF1-MCP-5M-ECM33_intron

    Plasmid

    #127600

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRS416-PTEF1-MCP-5P-ECM33_intron

    Plasmid

    #127599

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron with MS2 hairpins inserted near 5′ end of intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRS416-PTEF1-MCP-ECM33_intron

    Plasmid

    #127598

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRS416-PTEF1-MCP-no_intron

    Plasmid

    #127597

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    1xFLAG-MCP expression cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSMPUW_6.18_Ex_Ef1a-HA-EGFP-SV40-Zeo

    Plasmid

    #128454

    Purpose

    lentiviral expression vector with Ef1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeo Resistance gene

    Depositor

    Lindy Barrett

    Insert

    Ef1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. KA601: pMVP (L3-L2) pA; EF1a::TETa-pA

    Plasmid

    #121805

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + EF1a::TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. IY704: pMVP (L1-R5) EF1a promoter

    Plasmid

    #121693

    Purpose

    pMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    EF1a promoter
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. EF1a-3NLS-Sox2TALE-GCN4

    Plasmid

    #120548

    Purpose

    Express 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human SOX2 gene

    Depositor

    Yu Wang

    Article

    Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

    Insert

    NLS, Sox2 TALE, GCN4 (SOX2 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EF1a-3NLS-NGN2TALE-GCN4

    Plasmid

    #120547

    Purpose

    Express 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human NGN2 ene

    Depositor

    Yu Wang

    Article

    Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

    Insert

    NLS, NGN2 TALE, GCN4 (NEUROG2 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge