We narrowed to 13,072 results for: BASE
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Plasmid#135242PurposeGateway destination clone of GFP (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertGFP-V5
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianPromoterUbiquitinAvailable SinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 H2B-mIFP T2A Mpro (C145A)
Plasmid#163080PurposeExpresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2BDepositorInsertInactive Mpro mutant C145A and H2B-mIFP
ExpressionMammalianAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSG10-pGlnA-mCherry
Plasmid#102467PurposeExpresses mCherry fluorescent protein in E. coli. The expression is controlled by a pGlnA promoter.DepositorArticleInsertmCherry
ExpressionBacterialPromoterpGlnAAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRHA12
Plasmid#138942PurposeAND gate with IPTG and aTc as inputs and sfGFP as outputDepositorInsertriboregulated sfGFP
ExpressionBacterialAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-dsRed_IDG-K
Plasmid#135262PurposeGateway destination clone of dsRed (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-dsRed
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianPromoterUbiquitinAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSRG100 (germline optimized 3xGCN4 for C. elegans)
Plasmid#246457PurposeEncodes germline optimized 3xGCN4 for C. elegans. Useful to make CRISPR/Cas9 repair templates for 3xGCN4 integration in a gene of interest. For SunTag live-imaging of translation or protein labelling.DepositorInsert3xGCN4 (GLO)
ExpressionWormAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pGGD-ZmUBI Promoter-scFV-sfGFP
Plasmid#246802PurposeA GreenGate entry vector containing scFV-sfGFP fusion gene drived by an ZmUBI PromoterDepositorInsertZmUBI Promoter-scFV-sfGFP
UseGreengate cloning entry vectorAvailable SinceJan. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSELIS-SRTF1
Plasmid#246681PurposeUsed for growth-based enrichment of functional transcription factor variants and fluorescent-based readout of transcription factor function.DepositorInsertsSRTF1-responsive promoter
SRTF1-responsive promoter
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Circuit C44
Plasmid#245100PurposeRe-NF controllerDepositorInsertC44
ExpressionBacterialAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRHA40
Plasmid#138941PurposeXOR gate with IPTG and aTc as inputs and mRFP1 as outputDepositorInsertriboregulated mRFP1
ExpressionBacterialAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-hcRed-V5_IDG-K
Plasmid#135239PurposeGateway destination clone of hcRed (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInserthcRed-V5
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianPromoterUbiquitinAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-eGFP_IDG-K
Plasmid#135265PurposeGateway destination clone of eGFP (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-eGFP
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianPromoterUbiquitinAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSG05-pGlnA-deGFP
Plasmid#102462PurposeExpresses deGFP fluorescent protein in E. coli. The expression is controlled by a pGlnA promoter.DepositorArticleInsertdeGFP
ExpressionBacterialPromoterpGlnAAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.10
Plasmid#102919Purposeexpresses ABE7.10 in mammalian cellsDepositorInsertABE7.10
TagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-YE1-BE4max
Plasmid#198535PurposeExpresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-YE1-BE4max
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpRY-YE1-BE4max
Plasmid#198554PurposeExpresses human codon-optimized SpRY-YE1-BE4max and blasticidin resistance: EFS promoter-SpRY-YE1-BE4max-NLS-FLAG-P2A-BSDDepositorInsertSpRY-YE1-BE4max
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Cloning template vector
Plasmid#131471PurposeCloning template form ORANGE method based knock-in constructsDepositorTypeEmpty backboneTagsHAExpressionMammalianPromoterU6 and CbhAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-YE1-BE4max
Plasmid#198546PurposeExpresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-YE1-BE4max
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi3-91-NLuc
Plasmid#207150PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi2-91-NLuc
Plasmid#207149PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα13-125-NLuc
Plasmid#207155PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα12-133-NLuc
Plasmid#207156PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-SsAPOBEC3B
Plasmid#198536PurposeExpresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-SsAPOBEC3B
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-ABE8e(V106W)
Plasmid#198533PurposeExpresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-ABE8e(V106W)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDicAID_nCas9-PmCDA_NptII_Della
Plasmid#91694PurposeDicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDellaDepositorInsertSpCas9
TagsPmCDA1ExpressionPlantMutationD10A for nickase Cas9PromoterPcUbiAvailable SinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM5188
Plasmid#120087PurposeCyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.DepositorInsertpAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
UseOtherAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES
Plasmid#200402PurposeEntry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detectionDepositorInsertClosed-loop DART VADAR sensor entry cassette
UseSynthetic BiologyExpressionMammalianAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE6.3
Plasmid#102916Purposeexpresses ABE6.3 in mammalian cellsDepositorInsertABE6.3
TagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TmiR_G13-L_G12-E
Plasmid#25763PurposeEntry vector with TRE promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.DepositorInsertG alpha 13 and 12 miR-shRNA
UseEntry vectorAvailable SinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-BE3
Plasmid#113410PurposeExpresses hA3A-BE3 in mammalian cellsDepositorInserthA3A-BE3 (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-ABE8e(V106W)
Plasmid#198556PurposeExpresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-ABE8e(V106W)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-ABE8e(V106W)
Plasmid#198547PurposeExpresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-ABE8e(V106W)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpRY-ABE8e(V106W)
Plasmid#198553PurposeExpresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSDDepositorInsertSpRY-ABE8e(V106W)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
miniCGBE1 (pBM1219)
Plasmid#140253PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFPDepositorInsertBE4max(R33A)-ΔUGI
ExpressionMammalianMutationR33A in rAPOBEC1, D10A in Cas9PromoterCMVAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
miniABEMax V82G nSaCas9
Plasmid#135365PurposeExpression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitutionDepositorInsertminiABEMax V82G nSaCas9
UseCRISPRExpressionMammalianMutation(V82G)Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.9
Plasmid#102918Purposeexpresses ABE7.9 in mammalian cellsDepositorInsertABE7.9
TagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3B-BE3
Plasmid#113411PurposeExpresses hA3B-BE3 in mammalian cellsDepositorInserthA3B-BE3 (APOBEC3B S. pyogenes and Bacteriophage PBS2, Human)
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceOct. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS
Plasmid#89508PurposeExpression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.DepositorInsertrAPOBEC1-nCas9 (D10A)-UGI-NLS
TagsUGI and rAPOBEC-XTENExpressionBacterialMutationD10A mutation of Cas9PromoterT7Available SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCpf1-BE
Plasmid#107685PurposeExpresses dLbCpf1-BE in mammalian cellsDepositorInsertdLbCpf1-BE
ExpressionMammalianMutationD832A/E925A/D1148APromoterCMVAvailable SinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-YE1-BE4max
Plasmid#198549PurposeExpresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-YE1-BE4max
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_VCL sgRNA / hSpCas9
Plasmid#172837PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_TOMM20 sgRNA / hSpCas9
Plasmid#172836PurposeMammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniABEmax (pRZ900)
Plasmid#131311PurposeCMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).DepositorInsertbpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
ExpressionMammalianPromoterCMVAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES
Plasmid#200403PurposeEntry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detectionDepositorInsertClosed-loop DART VADAR sensor entry cassette
UseSynthetic BiologyExpressionMammalianAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCh-BICD2*-strep
Plasmid#120168PurposeExpresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidinDepositorInsertBICD cargo adaptor 2 (Bicd2 Mouse)
TagsStreptavidin and mCherryExpressionMammalianMutationTruncated BICD2: 15-595 aaPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.8
Plasmid#102917Purposeexpresses ABE7.8 in mammalian cellsDepositorInsertABE7.8
TagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-SsAPOBEC3B
Plasmid#198550PurposeExpresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-SsAPOBEC3B
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3D-BE3
Plasmid#113413PurposeExpresses hA3D-BE3 in mammalian cellsDepositorInserthA3D-BE3 (APOBEC3D S. pyogenes and Bacteriophage PBS2, Human)
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
G1333 DddAtox-N–SaKKH-Cas9(D10A)
Plasmid#157837Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertG1333 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
ExpressionMammalianAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
SECURE miniABEmax-V82G (pJUL1828)
Plasmid#131313PurposeCMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).DepositorInsertbpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
ExpressionMammalianPromoterCMVAvailable SinceSept. 28, 2019AvailabilityAcademic Institutions and Nonprofits only