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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 5,876 results for: nls

Showing: 1301 - 1350 of 5876 results

  1. pHR-IDR1-mCh-Cry2WT-NLS

    Plasmid
    #221918
    Purpose
    Expression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP IDR1 (aa 72-175) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the IDR1 region (aa 72 - 175); inser…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221918-plasmid-map-sequence-id-437702

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  2. L1P137 - pAHP6-PhiC31-NLS-tUBQ1

    Plasmid
    #219702
    Purpose
    Level 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integrase
    Depositor
    Jennifer Nemhauser
    Insert
    pAHP6:PhiC31-NLS:tUBQ1
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219702-plasmid-map-sequence-id-435732

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  5. pAAV-2xflox-BRX-eGFP-NLS

    Plasmid
    #217535
    Purpose
    AAV vector to drive the expression of eGFP in genetically defined cell populations under the control of the 4xBRE regulatory element of SMAD1 transcription factor and miniXon splicing casette in vivo
    Depositor
    Peter Scheiffele
    Article
    Okur et al bioRxiv 2023
    Insert
    4X BRE reporter and miniXon casette (Smad1 Mouse)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    BMP response element (BRE)
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    217535-plasmid-map-sequence-id-429503

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  18. CAGGS-safb∆sap-NLS-3XFLAG-V5

    Plasmid
    #196091
    Purpose
    Expresses mouse SAFB lacking the n-terminal SAP domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Use
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Removed amino acids 31-65 (SAP domain) of mouse S…
    Promoter
    CAGGS
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196091-plasmid-map-sequence-id-384675

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  19. CAGGS-safb∆dd1-NLS-3XFLAG-V5

    Plasmid
    #196092
    Purpose
    Expresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Use
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Removed amino acids 96-285 (predicted disordered …
    Promoter
    CAGGS
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196092-plasmid-map-sequence-id-384633

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  20. CAGGS-safb∆rrm-NLS-3XFLAG-V5

    Plasmid
    #196093
    Purpose
    Expresses mouse SAFB lacking the rrm domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Use
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Removed amino acids 428-506 (RRM) of mouse SAFB p…
    Promoter
    CAGGS
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196093-plasmid-map-sequence-id-386935

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  21. CAGGS-safb∆dd3-NLS-3XFLAG-V5

    Plasmid
    #196094
    Purpose
    Expresses mouse SAFB lacking the third computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Use
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Removed amino acids 638-925 (predicted disordered…
    Promoter
    CAGGS
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196094-plasmid-map-sequence-id-384649

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  22. CAGGS-SAFB-DD3-NLS-3XFLAG-V5

    Plasmid
    #196095
    Purpose
    Expresses only the third computationally called disordered domain of mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Use
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Contains only AA 619-925 (NLS and predicted disor…
    Promoter
    CAGGS
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196095-plasmid-map-sequence-id-384632

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  33. pICH47811-pAtAct2::Cas9-nls::tSlATPase

    Plasmid
    #160221
    Purpose
    Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse
    Depositor
    Matias Kirst
    Article
    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.
    Insert
    pAtACT2::Cas9-nls::tSlATPase
    Use
    Synthetic Biology; Moclo toolkit level 1 acceptor…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    160221-plasmid-map-sequence-id-316584

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  35. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2

    Plasmid
    #186413
    Purpose
    Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    lacZ (lacZ E. coli)
    Use
    Gateway destination vector
    Tags
    Nuclear localization signal
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186413-plasmid-map-sequence-id-369044

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  40. pKSB- PUb-NLSmTurqSV40 GGGG AAGA

    Plasmid
    #174535
    Purpose
    PUb-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Eric Marois
    Insert
    PUb-mTurquoise BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Aedes aegypti Polyubiquitin
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174535-plasmid-map-sequence-id-343229

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  41. pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL

    Plasmid
    #172208
    Purpose
    AAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1S promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.
    Depositor
    Garrett Kaas
    Article
    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d
    Insert
    HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
    Use
    AAV and Mouse Targeting; Tale
    Tags
    HA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172208-plasmid-map-sequence-id-338858

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  42. pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL

    Plasmid
    #172205
    Purpose
    AAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1FL promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.
    Depositor
    Garrett Kaas
    Article
    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d
    Insert
    HA-TALE(NG)-Tet1FL-SID4X-NLS_2A_EGFP_W3SL
    Use
    AAV and Mouse Targeting; Tale
    Tags
    HA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172205-plasmid-map-sequence-id-338845

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  47. pRDAX-EMMI-037 NLSNoLS(D-G)//Esp3I

    Plasmid
    #130239
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    NLSNoLS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130239-plasmid-map-sequence-id-249617

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Showing: 1301 - 1350 of 5876 results