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pcDNA 3.1(Hygro) myc-hPolQ-Flag

Plasmid

#73132

Purpose

To express in mammalian cells human PolQ

Depositor

Agnel Sfeir

Publication

Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

Insert

Polymerase theta (POLQ Human)

Tags

Flag and myc

Expression

Mammalian

Promoter

cmv promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA3 HA Arf6 DN T27N

Plasmid

#10831

Depositor

Thomas Roberts

Publication

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Arf6 T27N (ARF6 Human)

Tags

HA

Expression

Mammalian

Mutation

T27N. Defective in GTP binding. Dominant negati…

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLenti6-DEST PINK1-V5 KD

Plasmid

#13319

Depositor

Mark Cookson

Publication

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Kinase dead: K219A/D362A/D384A

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pWZL Neo Myr Flag FGFR1

Plasmid

#20486

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

FGFR1 (FGFR1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pBB540

Plasmid

#27393

Depositor

Bernd Bukau

Publication

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

grpE, clpB

Expression

Bacterial

Available since

April 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-MSP1D1deltaH5

Plasmid

#71714

Purpose

Membrane scaffold protein (MSP), helix 5 deletion

Depositor

Gerhard Wagner

Publication

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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inactive cdk1 FRET sensor (2328)

Plasmid

#26065

Depositor

Jonathon Pines

Publication

Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cdk1 FRET Sensor- Inactive

Tags

CFP variant, FLAG, and YFP variant

Expression

Mammalian

Mutation

Ser 126 mutated to Ala

Available since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-PDGFRB

Plasmid

#23893

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRB (PDGFRB Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pBabe-puro largeTcDNA

Plasmid

#14088

Depositor

William Hahn

Publication

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

Large T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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PL-SIN-EF1α-EGFP

Plasmid

#21320

Purpose

Control Lentiviral vector with strong EF1a promoter, expresses EGFP

Depositor

James Ellis

Publication

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

EF1α

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pJL-SaKKH-BE3

Plasmid

#85170

Purpose

Expresses SaKKH-BE3 in mammalian cells [Edit]

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SaKKH-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pET-15b-TEV-C9R

Plasmid

#118754

Purpose

TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

Depositor

Yutaka Kuroda

Publication

Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

Insert

Tobacco Etch Virus protease (NEWENTRY Synthetic)

Tags

C9R and His tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Cre Stoplight 2.4

Plasmid

#37402

Purpose

Cre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre

Depositor

Thomas Hughes

Publication

Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

Insert

Cre Stoplight 2.4

Tags

ZsGreen1 and mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYPQ203 (pMDC32-Ubi1)

Plasmid

#86207

Purpose

Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromycin resistance marker

Depositor

Yiping Qi

Publication

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Type

Empty backbone

Use

Gateway compatible attr1-attr2 destination vector

Expression

Plant

Promoter

Maize ubiquitin 1 (ZmUbi1)

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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tetO.Sox9.Puro

Plasmid

#117269

Purpose

Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Publication

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Tags

T2A and puromycin resistant cassette (C terminal …

Expression

Mammalian

Promoter

tet-on

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pUC57-mitoTPNOX

Plasmid

#87854

Purpose

This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

Depositor

Vamsi Mootha

Publication

Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

Insert

mitoTPNOX

Tags

C-terminal FLAG tag with a linker and mitochondri…

Expression

Bacterial

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EdTC

Plasmid

#24310

Depositor

Roel Nusse

Publication

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-dnhTcf4 // SV40-mCherry (TCF7L2 Human)

Use

Lentiviral

Tags

FLAG

Mutation

31 first amino acids of hTcf4 have been deleted.

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pcDNA3 Lats1 (Nigg HS189)

Plasmid

#41156

Depositor

Erich Nigg

Publication

Chan et al Oncogene. 2005 Mar 17;24(12):2076-86.

Insert

Lats1 (LATS1 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
pcDNA Flag-RARalpha

Plasmid

#35555

Depositor

Jonathan Kurie, Harish Srinivas

Publication

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RARalpha (RARA Human)

Tags

Flag and His

Expression

Mammalian

Available since

April 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDONR223-ALK

Plasmid

#23917

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ALK (ALK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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