59,541 results

1. Cdk5-HA

   Plasmid

   #1872

   Depositor

   Sander van den Heuvel

   Article

   van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

   Insert

   Cdk5 (CDK5 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIG-804_HA-GD2-28z_CAR_BATF-TFAP4

   Plasmid

   #207491

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   BATF-TFAP4, HA-GD2-28z_CAR (BATF Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG/ATP8B1-HA

   Plasmid

   #209209

   Purpose

   Mammalian expression of ATP8B1

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   ATP8B1 (ATP8B1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-Puro-TDP-43-A315T

   Plasmid

   #133755

   Purpose

   Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Changed alanine 315 to threonine

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-Sp1

   Plasmid

   #12097

   Depositor

   Robert Tjian

   Insert

   Sp1 (SP1 Human)
   

   Expression

   Mammalian

   Mutation

   aa1-87 deleted

   Available Since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. DsRed-rab7 DN

   Plasmid

   #12662

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB7 (Homo sapiens) (RAB7A Human)
   

   Tags

   DsRed and HA

   Expression

   Mammalian

   Mutation

   T22N

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBoBi-hTRPV4-C-GC6s

   Plasmid

   #154150

   Purpose

   To detect the calcium ion releases from TRPV4 channel

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

   Insert

   TRPV4 and GCamp6s (TRPV4 Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMaCTag-Z02

   Plasmid

   #120045

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TagBFP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   TagBFP
   

   Use

   Pcr template

   Tags

   TagBFP

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD

   Plasmid

   #197687

   Purpose

   plasmid for expressing the DNMT3bCD

   Depositor

   Michael Elowitz

   Article

   Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

   Insert

   H2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Synthetic, Human)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. hROSA26 CRISPR-pX330

    Plasmid

    #105927

    Purpose

    hROSA26 targeting CRISPR plasmid

    Depositor

    Masato Kanemaki

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    hROSA26 gRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-neo beta catenin S33Y

    Plasmid

    #16519

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    beta catenin (CTNNB1 Human)
    

    Expression

    Mammalian

    Mutation

    S33Y (SW48 mutation)

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDK521

    Plasmid

    #134204

    Purpose

    Baculovirus expresion of Astrin, LC8, and SKAP

    Depositor

    Iain Cheeseman

    Article

    Kern et al Elife. 2017 Aug 25;6. pii: 26866. doi: 1

    Insert

    Astrin 1-693-sGFP-His BroAstrin and LC8 and AltSKAP in pACE1
    

    Expression

    Insect

    Available Since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC7A5

    Plasmid

    #132197

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A5 (SLC7A5 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLJM1-B-GRAM-H-NeoR

    Plasmid

    #211709

    Purpose

    Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Lentiviral

    Tags

    ddFP-B3

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Leucine

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZW1-snoVector

    Plasmid

    #73174

    Purpose

    Nuclear expression of RNAs

    Depositor

    Ling-Ling Chen

    Article

    Yin et al Nucleic Acids Res. 2015 Jan;43(1):e5. do

    Inserts

    SNORD116-13 (SNORD116-13 Human)
    SNORD116-14 (SNORD116-14 Human)
    

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK286 (mAID-NeoR)

    Plasmid

    #72824

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Neo
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Available Since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. p-dCas9-JMJD2A-Hygro

    Plasmid

    #104410

    Purpose

    transient expression of dCas9-JMJD2A fusion protein

    Depositor

    Stephan Beck

    Insert

    JMJD2A (KDM4A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG mEmerald-EB3

    Plasmid

    #168514

    Purpose

    Expression of human plus-TIP microtubule binding protein EB3 fused with mEmerald for microtubule +end live tracking

    Depositor

    Franck Polleux

    Article

    Lewis TL et al Curr Biol. 2016 Oct 10;26(19):2602-2608.

    Insert

    EB3 (MAPRE3 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-Moesin-C-14

    Plasmid

    #55103

    Purpose

    Localization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Moesin (MSN Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pcsII-LMP7-flag

    Plasmid

    #86773

    Purpose

    C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    immunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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