We narrowed to 6,933 results for: CAD

1. pTMiniTrex-mCherry-Shark1

   Plasmid

   #233143

   Purpose

   Serves as a template plasmid for amplifying Shark1 (Tet-OFF) hammerhead aptazyme-3xTy-tagging cassette

   Depositor

   Ronald Etheridge

   Article

   Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

   Inserts

   mCherry
   Shark1
   

   Use

   Prokaryotic expression

   Tags

   3x Ty tag

   Expression

   Mutation

   Codon optimized for Trypanosoma cruzi and changed…

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA6-N-Myc-SCAP

   Plasmid

   #134285

   Purpose

   Expresses Myc-tagged SCAP in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SCAP (Scap, Scap Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. YFP NLS Beta-Actin

   Plasmid

   #60613

   Purpose

   Encodes YFP and NLS tagged beta-actin

   Depositor

   Primal de Lanerolle

   Article

   Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

   Insert

   Beta-actin (ACTB Human)
   

   Use

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV-CMVc-Cas9

   Plasmid

   #106431

   Purpose

   Expresses SpCas9 from the CMVc promoter in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   Cas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMVc

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lentiviral mBmal1-Luc

   Plasmid

   #182762

   Purpose

   Lentiviral vector encoding the promoter region of mouse Bmal1 gene driving a luciferase reporter

   Depositor

   Qing-Jun Meng

   Article

   Gossan et al Arthritis Rheum. 2013 Sep;65(9):2334-45.

   Insert

   mouse arntl promoter (Bmal1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   649 bp

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. G-GECO1.2-Orai1

   Plasmid

   #73562

   Purpose

   Green fluorescent reporter of Orai1-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   G-GECO1.2-Orai1 (ORAI1 Synthetic, Human)
   

   Use

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Immediate Early

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGST-DHFR Mm

   Plasmid

   #83073

   Purpose

   Expresses the full length DHFR Mm in E.coli

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   2x DHFR (Dhfr Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG104 FAST

   Plasmid

   #130720

   Purpose

   Expresses FAST (also called YFAST) in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   FAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUNIV-SIG-5HTR4D100A

   Plasmid

   #16312

   Depositor

   Bruce Conklin

   Article

   Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

   Insert

   h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (HTR4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Added FLAG tag; Aspartic Acid 100 changed to Alan…

   Promoter

   Available since

   Jan. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pmCherryC2-Gephyrin P1

    Plasmid

    #68820

    Purpose

    mCherry tagged Geph for mammalian expression

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al Proc Natl Acad Sci U S A. 2011 Jan 4;108

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    silent mutations on internal EcoRI site. Gephyrin…

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSicoR-Ef1a-mCh-Puro

    Plasmid

    #31845

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SunTagng14aa

    Plasmid

    #106439

    Purpose

    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. yHS1-M7A,H102A

    Plasmid

    #159152

    Purpose

    Cytosolic labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    yHS1-M7A,H102A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pDB-hCRY1-mScarlet-I-CD4-bla

    Plasmid

    #179442

    Purpose

    Donor vector to knock in mScarlet-I C-terminal to human CRY1 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    mScarletI
    

    Use

    CRISPR

    Tags

    His/Flag

    Expression

    Mutation

    Promoter

    None

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5-EGFP-AID-BubR1

    Plasmid

    #47330

    Purpose

    for plasmid integration using the Flp-In system. AID at N-terminal

    Depositor

    Don Cleveland

    Article

    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

    Insert

    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    

    Use

    Tags

    AID and GFP

    Expression

    Mammalian

    Mutation

    siRNA resistant

    Promoter

    Available since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Orai1-GCaMP6f

    Plasmid

    #73564

    Purpose

    Green fluorescent reporter of Orai1-associated calcium influx

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

    Insert

    Orai1-GCaMP6f (ORAI1 Synthetic, Human)
    

    Use

    Tags

    GCaMP6f

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Immediate Early

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-CAG-NanoLuc-VChR1-EYFP

    Plasmid

    #114112

    Purpose

    fusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    NanoLuc; Volvox channelrhodopsin 1; EYFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLDLR-Luc mutSRE

    Plasmid

    #14945

    Depositor

    Axel Nohturfft

    Article

    Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    LDLR promoter mut SRE (LDLR Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Point mutation in the SRE-1 sequence of the LDL r…

    Promoter

    Available since

    May 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. [pSK24] pACYCDuet His-c7orf59|HBXIP

    Plasmid

    #136143

    Purpose

    bacterial expression of c7orf59 and HBXIP

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    c7orf59 and HBXIP (LAMTOR4, LAMTOR5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA6-N-Flag-SREBP1

    Plasmid

    #134283

    Purpose

    Expresses Flag-tagged SREBP1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP1 isoform a precursor

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge