161,273 results

1. pC019 - LwCas13a from Leptotrichia wadei

   Plasmid

   #91909

   Purpose

   Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a and guide expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. DV-GFP-Rab38-WT-pDEST53

   Plasmid

   #15669

   Depositor

   William Pavan

   Article

   Loftus et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

   Insert

   Rab38 (Rab38 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   wildtype Rab38 sequence

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-N2cG

   Plasmid

   #100811

   Purpose

   express rabies virus CVS N2c strain glycoprotein gene (N2cG)

   Depositor

   Ian Wickersham

   Insert

   rabies virus CVS N2c strain glycoprotein gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPAGFP-VSVG

   Plasmid

   #11915

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

   Insert

   VSVG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   (L64F/T65S/V163A/T203H). The PAGFP also contains …

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcCas12nHS_RV6_empty

   Plasmid

   #203811

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189923

   Purpose

   AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SaABE8e V106W
   

   Use

   AAV

   Tags

   Expression

   Mutation

   nSaCas9 KKH, TadA ABE8e V106W

   Promoter

   EFS

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ADAMTS1_pCSdest

   Plasmid

   #53807

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   ADAMTS1 (ADAMTS1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pLSSmKate1-C1

   Plasmid

   #31868

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   LSS-mKate1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

   Promoter

   CMV

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCytERM_mScarlet_N1

   Plasmid

   #85066

   Purpose

   In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   CytERM
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   aa1-29

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHygR2r

    Plasmid

    #200072

    Purpose

    hygR selection nls-mScarlet marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHygG2r

    Plasmid

    #200069

    Purpose

    hygR selection nls-mNG marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pALPS vpu

    Plasmid

    #101330

    Purpose

    Encodes codon optimized NL4-3 vpu

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 vpu
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR_Hu_BIRC5(WT)

    Plasmid

    #136351

    Purpose

    Human BIRC5 cDNA in Gateway pENTR

    Depositor

    Guillermo de Cárcer

    Insert

    BIRC5 (BIRC5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xMma PylT_FLAG-Mma PylRS

    Plasmid

    #140009

    Purpose

    Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. AiP15578 - pAAV-AiE0780m-minBG-iCre(R297T)-BGHpA (Alias: CN5578)

    Plasmid

    #224200

    Purpose

    AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D1 MSNs

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    iCre(R297T)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-SpRY-HF1-ABE8e

    Plasmid

    #185670

    Purpose

    Expresses SpRY-HF1-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-nSpRY-HF1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SpRY-HF1 D10A

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

    Plasmid

    #149722

    Purpose

    Lentiviral expression of crRNA with GFP and a neomycin resistance gene

    Depositor

    Junwei Shi

    Article

    Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMEXNH3

    Plasmid

    #110079

    Purpose

    Analogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    His10 Tag - 3C cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Dronpa3-N1

    Plasmid

    #54682

    Purpose

    Localization: N1 Cloning Vector, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    Dronpa3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pX330-PITCh-H2BC11

    Plasmid

    #207755

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBA407

    Plasmid

    #85970

    Purpose

    UPRE-mCh-Ubc-Neo

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    mCherry
    Neo
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLG166

    Plasmid

    #124366

    Purpose

    Gfpmut2 constitutively expressed using the Ppen promoter for expression in B. subtilis

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    gfpmut2 (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppen

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-hSynapsin1-GCaMP6s-P2A-mKate2

    Plasmid

    #112006

    Purpose

    For co-expression of GCaMP6s with mKate2

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV9

    Insert

    GCaMP6s-P2A-mKate2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.3-SARS2-B.1.617.2

    Plasmid

    #172320

    Purpose

    expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike of B.1.617.2 strain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

    Promoter

    CMV-F

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-EF1a-DIO-GRAB_g5-HT3.0

    Plasmid

    #208726

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3/hArf3(WT)-mCherry

    Plasmid

    #79420

    Purpose

    Expresses C-terminally mCherry-tagged Arf3(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf3 (ARF3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJBM4

    Plasmid

    #225291

    Purpose

    E. coli-Campylobacter shuttle vector; TetR

    Depositor

    Robert Moore

    Article

    McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

    Insert

    Tet(O) and its putative promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMT899

    Plasmid

    #228288

    Purpose

    Constitutive mutant rPhlTA expression with strong promoter

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    rphlTA mutant
    

    Use

    Synthetic Biology

    Tags

    Three copies of VP16 (VP48)-Nuclear localization …

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K

    Promoter

    KpGAPDH promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pnEA-vH_His-TEV-SEPT2-msfGFP_SEPT6

    Plasmid

    #174498

    Purpose

    bacterial co-expression of human SEPT2 fused to monomeric superfolder GFP and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Use

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET_His6-SUMO-TEV-TtCsm6

    Plasmid

    #172486

    Purpose

    Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
    

    Use

    Tags

    His-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter and lac operator

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid

    #208048

    Purpose

    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    BirAopt
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    BirAopt is human-codon-optimized BirA (PMID 15707…

    Promoter

    EFS

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. H3K9me3Probe_FullLength

    Plasmid

    #186463

    Purpose

    Expresses GFP tagged H3K9me3 recognition motif in mammalian cells.

    Depositor

    Chongli Yuan

    Article

    Mendonca et al Cytometry A. 2022 Apr;101(4):339-350. do

    Insert

    dimeric CDY1 (CDY1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBac-DsRed-ORCO_9kbProm-QF2

    Plasmid

    #104877

    Purpose

    Anopheles gambiae ORCO enhancer/promter to drive QF2 expression in Anopheles transgenic mosquitoes

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

    Insert

    AgORCO-QF2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAV-U6+27-Tornado-BroccoliSamSensor#1

    Plasmid

    #124363

    Purpose

    Overexpression of circular RNA (Broccoli-based SAM sensor)

    Depositor

    Samie Jaffrey

    Article

    Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

    Insert

    Tornado-BroccoliSamSensor#1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTriEx4-Rac1-2G

    Plasmid

    #66110

    Purpose

    Second generation Rac1 FRET biosensor

    Depositor

    Olivier Pertz

    Article

    Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

    Insert

    Second generation Rac1 FRET biosensor
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEGFP-N1 hMOG-alpha1-EGFP

    Plasmid

    #126463

    Purpose

    Expression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells

    Depositor

    Markus Reindl

    Article

    Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

    Insert

    Myelin oligodendrocyte glycoprotein (MOG Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. RUNX1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176974

    Purpose

    Mammalian expression vector encoding RUNX1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTR-346 pUC19-tNGFR-P2A-IL2RA N

    Plasmid

    #186065

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL2RA (IL2RA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Bxb1-GT_CAG-mRuby2

    Plasmid

    #229801

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFsynW KDEL Hook

    Plasmid

    #197277

    Purpose

    Encodes ER-targeted streptavidin-KDEL hook (RUSH system).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    Streptavidin
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge