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174,739 results

Showing: 10841 - 10880 of 174739 results

  6. pXPR_118

    Plasmid
    #113667
    Purpose
    Lentiviral vector expressing dCas9
    Depositor
    John Doench
    Insert
    dCas9
    Use
    CRISPR and Lentiviral
    Mutation
    catalytically inactive
    Available Since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113667-plasmid-map-sequence-id-220921

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  9. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid
    #102886
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102886-plasmid-map-sequence-id-194712

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  10. pDNA2.0 6H-FLAG-TEV-KRAS G12C

    Plasmid
    #111848
    Purpose
    Expresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Article
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G12C (KRAS Human)
    Tags
    6xHis-tag, FLAG-tag, and TEV protease cleavage se…
    Expression
    Bacterial
    Mutation
    Changed Glycine 12 to Cysteine
    Promoter
    T5
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111848-plasmid-map-sequence-id-213454

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  11. AAV.rTH.PI.Cre.SV40

    Plasmid
    #107788
    Purpose
    AAV vector expressing Cre from rTH promoter
    Depositor
    James M. Wilson
    Has Service
    AAV Retrograde and AAV9
    Insert
    Cre
    Use
    AAV
    Expression
    Mammalian
    Promoter
    rTH
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    107788-plasmid-map-sequence-id-259405

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  15. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

    Plasmid
    #105448
    Purpose
    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector
    Depositor
    Hillel Adesnik
    Article
    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:
    Has Service
    AAV9
    Insert
    ChrimsonR-mRuby2-ST
    Use
    AAV
    Tags
    mRuby2 and soma targeting motif
    Mutation
    K176R in Chrimson
    Promoter
    hSyn
    Available Since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105448-plasmid-map-sequence-id-448714

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  19. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40

    Plasmid
    #100833
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6f
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G
    Promoter
    Synapsin
    Available Since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    100833-plasmid-map-sequence-id-198271

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  20. pcDNA3-neo-cterminal-3HA

    Plasmid
    #102643
    Purpose
    To generate three copies of the HA epitope tag on cDNAs.
    Depositor
    Michael E. Wright
    Type
    Empty backbone
    Tags
    3x HA (YPYDVPDYA)
    Expression
    Mammalian
    Available Since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    102643-plasmid-map-sequence-id-196911

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  21. pDIRECT_25H

    Plasmid
    #91145
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91145-plasmid-map-sequence-id-182281

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  23. AbVec2.0-IGHG3

    Plasmid
    #99577
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG3 isotype
    Depositor
    Hedda Wardemann
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    hCMV IE1 gene promoter
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    99577-plasmid-map-sequence-id-192592

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  25. pCRISPRi_Mxi1_yl

    Plasmid
    #91248
    Purpose
    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    91248-plasmid-map-sequence-id-192174

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  28. pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE

    Plasmid
    #89609
    Purpose
    over-expression of VEGF-A(165) in human cells
    Depositor
    Fernando Fierro
    Insert
    VEGF-A(165) (VEGFA Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    K188E
    Promoter
    MNDU3
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    89609-plasmid-map-sequence-id-183289

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  29. pDIRECT_21C

    Plasmid
    #91130
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91130-plasmid-map-sequence-id-182254

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Showing: 10841 - 10880 of 174739 results