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Accessory plasmid 2

Plasmid

#128571

Purpose

for cloning identified individual SPECS

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagRFP

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - DDX3Y sgRNA 2

Plasmid

#70657

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against DDX3Y

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only
pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

Plasmid

#71213

Purpose

dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A

Tags

dSpCas9-BFP-DNMT3A(E752A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-ARAP3(R307,8A)

Plasmid

#39486

Depositor

Sonja Vermeren

Article

Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

Insert

ARAP3(R307,8A) (ARAP3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R307,8A = PH domain mutation

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pC3.1_CAG_oROS-Gr

Plasmid

#216113

Purpose

Expresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv [Preprint]. 2024 Feb 4:2024.01.3

Insert

CAG_oROS-Gr

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMJ329: pCMV-MmeFz2

Plasmid

#205266

Purpose

Human expression vector for human codon optimized MmeFz2

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

MmeFz2

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMH-EF1-GFP

Plasmid

#133018

Purpose

(Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under EF1 promoter.

Depositor

Michael Huen

Article

Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

Type

Empty backbone

Tags

GFP

Expression

Mammalian

Promoter

EF1

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCIneoLuc-PP2A

Plasmid

#128349

Purpose

Vector for LUMIER assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

PP2A (PTPA Human)

Tags

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2_ISCro4_Inversion_Reporter

Plasmid

#251499

Purpose

Reporter plasmid for monitoring inversion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL components

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 inversion reporter

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pBLO1811_Cas9_noNLS_human

Plasmid

#74490

Purpose

Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

Cas9

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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5013 TRV2-eTnpBc-reRNA4ChlH

Plasmid

#251824

Purpose

Editing H subunit of magnesium chelatase (ChlH) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

Enhanced variant of ISDra2 eTnpBc with guide targeting NbChlH with HDV at 3' end

Tags

AtFDnls

Expression

Plant

Mutation

N4Y/R110K/V192L/L222I

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pMCP-MU-Luc (MCP-1 mut promoter in pGL3-basic)

Plasmid

#40325

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

MCP-1 promoter (mutated) (Ccl2 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

contains MCP-1 promoter region through the nucleo…

Promoter

MCP-1

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pGV7 (CK2alpha' K69M, CK2beta)

Plasmid

#27095

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha' K69M (CSNK2A2 Human)

Use

Tetracycline-regulated expression

Tags

HA tag on CK2alpha' and Myc on CK2beta

Expression

Mammalian

Mutation

K69M on CK2alpha'

Promoter

bidirectional tet-responsive promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pGEL685

Plasmid

#205592

Purpose

NEPE for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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LentiV2-mU6-evopreQ1

Plasmid

#210190

Purpose

pegRNA/ngRNA library cloning

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Type

Empty backbone

Use

Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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1457_pDEST_miniTol2_R4-R2_Cryst_mCherry

Plasmid

#171796

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pv6_MBD1_MBD

Plasmid

#179404

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1_MBD

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJAK112

Plasmid

#167280

Purpose

CodA-based mutagenesis vector for C. difficile

Depositor

Robert Fagan

Article

Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

Type

Empty backbone

Use

E. coli - c. difficile shuttle vector

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GFP

Plasmid

#158130

Purpose

Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

sfGFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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JB84

Plasmid

#128908

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab9AQL-BirA*-HA-MAO (RAB9A Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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