We narrowed to 32,968 results for: cmv

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pCMV-WDR5-P2A-SH2-P2A-Ras-CAAX

Plasmid

#209712

Purpose

Mammailan expression of WDR5, SH2, and HRas separated by P2A.

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

WDR5-P2A-SH2-P2A-Ras-CAAX

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgNotch2#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209069

Purpose

Entry vector that encodes sgRNAs against mouse Notch2, Rb1, Trp53, Rbl2, and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Hong et al Cancer Res. 2022 Jan 15;82(2):248-263. d

Insert

sgRNAs targeting Notch2, Rb1, Trp53, Rbl2 and Cre recombinase

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgNotch1#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209068

Purpose

Entry vector that encodes sgRNAs against mouse Notch1, Rb1, Trp53, Rbl2, and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Hong et al Cancer Res. 2022 Jan 15;82(2):248-263. d

Insert

sgRNAs targeting Notch1, Rb1, Trp53, Rbl2 and Cre recombinase

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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EK0544 CMV-TO-mCherry-SG(s70587-dblstop)SG-EGFP (FLP-IN)

Plasmid

#191176

Purpose

Mammalian expression of EK0209 with a second stop codon.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

CMV-TO:mCherry:s70587:EGFP (double-stop)

Expression

Mammalian

Mutation

additional stop codon

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0425 CMV-TO-BFPmut-stop-Bdnf.t+54@202 (FLP-IN)

Plasmid

#191147

Purpose

Expression of the sequence matching the 54 extra bases in EK0362 in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

Bdfn.t+54

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC1584 - pscAAV CMV-IE secreted EGFP-THPKTEL WPRE

Plasmid

#188539

Purpose

An AAV packaging vector that expresses secreted C-CDNF under control of the EGFP promoter.

Depositor

Brandon Harvey

Insert

secreted EGFP

Use

AAV

Tags

CDNF(1-28) and THPKTEL WPRE

Promoter

CMV-IE

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670

Plasmid

#169592

Purpose

Tier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

phloretin-controlled SEAP and iRFP expression

Expression

Mammalian

Promoter

TtgO2-PCMVmin-2

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pVH269-Tier1-OVanO2-PCMVmin2-SEAP-p2A-iRFP670

Plasmid

#169667

Purpose

Tier-1 vector encoding PVanO2-driven SEAP-p2A-iRFP670 expression (OVanO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

vanillic acid-controlled SEAP and iRFP expression

Expression

Mammalian

Promoter

VanO2-PCMVmin

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1

Plasmid

#182380

Purpose

Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

mtIF3 (Mtif3 Mouse)
mito-mTFP1

Tags

3xFlag

Expression

Mammalian

Mutation

Changed CDS sequence from 'ccacgttcaagtcacga…

Promoter

CMV promoter and SV40 promoter

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-Lyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVp

Plasmid

#179672

Purpose

Membrane-bound (Lyn) TEVP-inducible caged-cTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged cTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-B2AD-SpyTag-tvmvs-KKMP

Plasmid

#179648

Purpose

TVMVP-inducible single TM SEAP RELEASE construct with SpyTag

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-nTVMVp-AP4-tevs-P3-tevs-cTVMVPmut-CAAX

Plasmid

#179662

Purpose

Membrane-bound (CAAX) TEVP-inducible caged-nTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged-nTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26sFur-B2AD-hcvs-tevs-KKMP

Plasmid

#179643

Purpose

OR Gate (TEVP/HCVP) SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-EGFP-PolB(K72A)-PAMmut-Hygro

Plasmid

#176087

Purpose

EGFP fused to the N-terminus of POLB containing the mutation Lys72Ala, a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutation in Lys72 to Ala, and mutation in the PAM…

Promoter

CMV

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-10A.12D-48-3xFLAG

Plasmid

#130678

Purpose

Expresses human SMCHD1 Exon1-10A.12D-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-10A.12D-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D409_F489del

Promoter

CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)

Plasmid

#133798

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized AcrIIA1(T114A/F115A)

Tags

NLS(SV40)

Expression

Mammalian

Mutation

T114A/F115A

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2

Plasmid

#91802

Purpose

Expresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be used

Depositor

Jeremy Wilusz

Article

Liang et al Methods. 2021 Apr 18. pii: S1046-2023(21

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-3xHA-SMCHD1-Exon1-14.47M-48-3xFLAG

Plasmid

#130691

Purpose

Expresses human SMCHD1Exon1-14.47M-48 with an N-terminal 3xHA tag and a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-14.47M-48 (SMCHD1 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

E653_G1960del

Promoter

CMV

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG

Plasmid

#130690

Purpose

Expresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM, G1720_Y1846del

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6AP III (C41A, C46A, C840A)

Plasmid

#119318

Purpose

lentiviral vector for expression of HA-Tagged E6-AP III (C41A, C46A, C840A) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP III (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

C41A, C46A, C840A

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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