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siRNA resistant ABHD16A-S176AS306A-mNG

Plasmid

#195165

Purpose

mammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant ABHD16A-H189AD194A-mNG

Plasmid

#195166

Purpose

mammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant ABHD16A-S355A-mNG

Plasmid

#195167

Purpose

mammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant ABHD16A-S355A-Halo

Plasmid

#195169

Purpose

mammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tag

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Use

Tags

Halo tag

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP cLAP -1

Plasmid

#194512

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLAP(EGFP)-P2A-NGFR

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP cLAP -0

Plasmid

#194511

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLAP(EGFP)-P2A-NGFR

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP cLAP +1

Plasmid

#194509

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLAP(EGFP)-P2A-NGFR

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP nLAP -1

Plasmid

#194506

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

NGFR-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP cLAP +0

Plasmid

#194508

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLAP(EGFP)-P2A-NGFR

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag NGFR EGFP nLAP +1

Plasmid

#194315

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

NGFR-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP nLAP +0

Plasmid

#194314

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

NGFR-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA424::tsi5

Plasmid

#192960

Purpose

Expresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organisms

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tsi5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET29a(+)::D1141A

Plasmid

#192963

Purpose

Test the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGL

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

Tse5_D1141A

Use

Tags

9xhis + TEV tag

Expression

Bacterial

Mutation

D1141A

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET29a(+)::9xhis-Tse5

Plasmid

#192962

Purpose

Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

Tse5

Use

Tags

9xhis + TEV tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pRD415

Plasmid

#178197

Purpose

Rok _Linker overexpression

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

rok _linker

Use

Tags

Expression

Bacterial

Mutation

deletion of residues 75-96

Promoter

T7

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRD461

Plasmid

#178198

Purpose

Rok with a C-terminal His-tag overexpression

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

rok 6xHis

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCALNL-tdTomato-2A-mSyp::EGFP

Plasmid

#191213

Purpose

Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

Depositor

Theofanis Karayannis

Article

Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

Insert

tdTomato-T2A-mSyp::EGFP (Syp Mouse)

Use

Pcagen

Tags

mSyp::EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pIPKAH

Plasmid

#185869

Purpose

Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)

Depositor

Claudia Vickers

Article

Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

Insert

GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJCble

Plasmid

#185861

Purpose

The CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable marker

Depositor

Claudia Vickers

Article

Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

Insert

PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; ble

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pNCS-Clover4-LUMABS-HA

Plasmid

#183486

Purpose

To express the Clover4-LUMABS-HA sensor for Avian Influenza Virus antibody detection.

Depositor

Jun Chu, Zongwen Jin

Article

Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

Insert

Clover4-LUMABS-HA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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