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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,759 results for: nls

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Showing: 10881 - 10920 of 18759 results

  1. pCMV-AAVS1-TALEN-R

    Plasmid
    #159300
    Purpose
    TALEN (right) targeting the AAVS1 locus with obligate heterodimer FokI
    Depositor
    Kosuke Yusa
    Insert
    TALEN targeting the AAVS1
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    159300-plasmid-map-sequence-id-311596

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  3. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

    Plasmid
    #140193
    Purpose
    Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dLbCas12a-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta), NLS
    Mutation
    D832A DNAse deactivated
    Promoter
    PgpdA and PtrpC
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140193-plasmid-map-sequence-id-309648

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  7. Lentiviral nontargeting shRNA RFP i670

    Plasmid
    #155287
    Purpose
    Lentiviral expression of nontargeting shRNA, RFP i670 expression, based on Addgene 12247
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Expression
    Mammalian
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155287-plasmid-map-sequence-id-310003

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  9. pRSV-p65T305A

    Plasmid
    #106454
    Purpose
    NF-kB p65, kinase site 305 mutated
    Depositor
    Georg Weber
    Publication
    He et al Eur J Biochem. 2003 May . 270(10):2174-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NF-kB p65, kinase site 305 mutated (RELA Human)
    Expression
    Mammalian
    Mutation
    kinase site inactivated
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    106454-plasmid-map-sequence-id-207854

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  17. pSCIP-hTRAC

    Plasmid
    #154094
    Purpose
    Self-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain
    Depositor
    Scott McComb
    Publication
    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA and BAIT targeting human TRAC locus
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    154094-plasmid-map-sequence-id-307107

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  19. dSp-VP64-RTA

    Plasmid
    #99675
    Purpose
    Expresses dSp Cas9 fused at C term. To VP64 and RTA activation domains
    Depositor
    George Church
    Publication
    Vora et al bioRxiv 2018. 298620
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Tags
    VP64-RTA
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    99675-plasmid-map-sequence-id-192088

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  28. dSp-VP64-p65-RTA(75-190)

    Plasmid
    #99670
    Purpose
    Expresses dSp Cas9 fused to combination of truncated activation domains
    Depositor
    George Church
    Publication
    Vora et al bioRxiv 2018. 298620
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Tags
    VP64-p65-RTA(75-190)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available since
    May 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    99670-plasmid-map-sequence-id-192065

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  29. pTJK644

    Plasmid
    #138012
    Purpose
    SIX1 knockout
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA2 (SIX1 Human)
    Use
    CRISPR and Lentiviral
    Mutation
    None
    Promoter
    U6
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138012-plasmid-map-sequence-id-275045

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  32. pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

    Plasmid
    #140228
    Purpose
    RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.
    Depositor
    Stanley Qi
    Publication
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    Use
    Lentiviral
    Tags
    BFP
    Expression
    Mammalian
    Promoter
    RRM2 endogenous promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140228-plasmid-map-sequence-id-291990

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  34. p-dCas9-dDNMT3a-C706S-Hygro

    Plasmid
    #104412
    Purpose
    transient expression of dCas9-dDNMT3a fusion protein
    Depositor
    Stephan Beck
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dDNMT3a
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    C706S
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104412-plasmid-map-sequence-id-204224

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Showing: 10881 - 10920 of 18759 results